General Information

Product

Capricious Antibody Affinity Purified

Description

Affinity Purified Capricious, isoform A Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide corresponding to 400-450 on Capricious isoform A protein.

Accession #

NCBI:AAF49837

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q9VU53

Overview

Invovled in axon guidance, branch fusion, opening tracheal system, synapse assembly and others.

Subcellular Location

Muscle cell projection

Structure

Contains Leucine-rich repeat

Alternative Nomenclature

BcDNA:LD07388 antibody

caps antibody

CG11282 antibody

CT31495 antibody

Dmel\CG11282 antibody

l(3)02937 antibody

l(3)rM632 antibody

General Information

Product

inaD Antibody

Description

Affinity Purified inactivation no afterpotential D, isoform A Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, WB

Host

Rabbit

Species Cross Reactivity

Fruity Fly (Drosophila melanogaster)

Immunogen

Synthetic peptide corresponding to C terminal amino acids 659-674 of Fruit fly (Drosophila melanogaster) inaD protein.

Sequence:

EVTRPKPTLRTEAPKA

Accession #

NCBI:  NP_726260.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q24008

Overview

Involved in the negative feedback regulation of the light-activated signaling cascade in photoreceptors through a calcium-mediated process.

Molecular Function

Calmodulin binding, myosin III binding, receptor signaling complex scaffold activity, structural molecule activity, myosin binding, photoreceptor activity

Subcellular Location

Cell membrane, preipheral membran protein

Expression

Expressed in rhabdomeres of the compound eyes and ocelli.

Alternative Nomenclature

Inactivation-no-after-potential D protein antibody

inaD antibody

General Information

Product

Phospho Smad1 Antibody

Description

Affinity Purified Protein mothers against dpp Antibody

Verified Applications

ELISA, ICC/IF, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 405-455 on D. melanogaster Mothers against dpp protein.

Accession #

NCBI:  NP_477017.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.0 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

P42003

Overview

Required for the function of decapentaplegic. May play an important role in mediating Dpp signaling. Involved in the BMP signaling pathway.

Molecular Function

DNA binding; RNA polymerase II activating transcription factor; Transcription regulatory region sequence-specific activity; Transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity

Subcellular Location

Cytoplasm; Nucleus

Expression

Is detected in all developmental stages, though it appears most abundant in pupae, adult stages and early embryos. Its abundance decreases throughout embryonic and larval development and then returns to high levels in pupae and adult females.

Structure

Homotrimer

Alternative Nomenclature

BSP 1 antibody

Dwarfin A antibody

DwfA antibody

JV 41 antibody

MAD homolog 1 antibody

MAD mothers against decapentaplegic homolog 1 antibody

Mad related protein 1 antibody

MADR1 antibody

Mothers against DPP homolog 1 antibody

Sma and Mad related protein 1 antibody

SMAD 1 antibody

Spinal muscular atrophy distal with upper limb predominance antibody

TGF beta signaling protein 1 antibody

Transforming growth factor beta signaling protein 1 antibody

General Information

Product

Protein Vestigial Antibody Affinity Purified

Description

Affinity Purified Vestigial Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 200-250 on D. melanogaster Vestigial protein.

Accession #

NCBI:  NP_523723.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q26366

Overview

Involved in determining which thoracic imaginal disk cells will form wings and halteres, perhaps by interacting with other nuclear regulatory proteins. When in combination with scalloped (sd), it acts as a transcriptional activation complex that regulates gene expression in the wing. Binding to sd switches the DNA target selectivity of sd. Required and sufficient for cell proliferation at the dorsal/ventral (D/V) boundary of the wing imaginal disk. Also required for cell proliferation in the wing imaginal disk, mediated via activation of E2f. By interacting with Dhfr, may control genes involved in DNA replication.

Molecular Function

DNA binding; Protein C-terminus binding

Subcellular Location

Nucleus

Expression

Expressed in the developing wing primordia initially along the D/V wing boundary, and by the late third larval instar, maximal expression is seen in cells at the D/V wing disk boundary. Less expression is seen in cells located farther from this boundary.

Alternative Nomenclature

VG

VSTGL

VG21

DMEL/CG3830

CCG3830

General Information

Product

DRP1 Antibody

Description

Dynamin-related Protein 1 Antibody

Verified Applications

ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

D. Melanogaster, Human, Mouse, Rat

Immunogen

Synthetic peptide within 450-490 on human DRP1 protein.

Accession #

GenBank: BAC06576.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.68 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:450

Protein

Uniprot #

Q8K1M6

Overview

Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism.

Molecular Function

GTP-binding, Lipid-binding, Nucleotide-binding

Subcellular Location

Golgi apparatus; Peripheral membrane protein; peroxisome; Mitochondrion outer membrane; Clathrin-coated pit; synaptic vesicle membrane

Expression

Expressed in the cerebellum and in several regions of the cerebrum and diencephalon

Structure

Homotetramer; dimerizes through the N-terminal GTP-middle region of one molecule binding to the GED domain of another DNM1L molecule. Oligomerizes in a GTP-dependent manner to form membrane-associated tubules with a spiral pattern. Can also oligomerize to form multimeric ring-like structures.

Alternative Nomenclature

DLP1 antibody

dnm1l antibody

Dnm1p/Vps1p-like protein antibody

dnml1 antibody

DRP1 antibody

DVLP antibody

Dymple antibody

Dynamin 1 like antibody

Dynamin family member proline-rich carboxyl-terminal domain less antibody

Dynamin like protein antibody

Dynamin-like protein 4 antibody

Dynamin-related protein 1 antibody

DYNIV 11 antibody

EMPF antibody

FLJ41912 antibody

HdynIV antibody

VPS1 antibody

General Information

Product

Smad1 Antibody Affinity Purified

Description

Affinity Purified Mothers against dpp Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 405-455 on D. melanogaster Mothers against dpp protein.

Accession #

NCBI:  NP_477017.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.0 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P42003

Overview

Required for the function of decapentaplegic. May play an important role in mediating Dpp signaling. Involved in the BMP signaling pathway.

Molecular Function

DNA binding; RNA polymerase II activating transcription factor; Transcription regulatory region sequence-specific activity; Transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity

Subcellular Location

Cytoplasm; Nucleus

Expression

Is detected in all developmental stages, though it appears most abundant in pupae, adult stages and early embryos. Its abundance decreases throughout embryonic and larval development and then returns to high levels in pupae and adult females.

Structure

Homotrimer

Alternative Nomenclature

BSP 1 antibody

Dwarfin A antibody

DwfA antibody

JV 41 antibody

MAD homolog 1 antibody

MAD mothers against decapentaplegic homolog 1 antibody

Mad related protein 1 antibody

MADR1 antibody

Mothers against DPP homolog 1 antibody

Sma and Mad related protein 1 antibody

SMAD 1 antibody

Spinal muscular atrophy distal with upper limb predominance antibody

TGF beta signaling protein 1 antibody

Transforming growth factor beta signaling protein 1 antibody

General Information

Product

MEDEA Antibody Affinity Purified

Description

Affinity Purified Medea Antibody

Verified Applications

DB, ELISA, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 150-250 on Drosophila melanogaster Medea protein.

Accession #

GenBank: AAC62005.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.73 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q7KPL1

Overview

Sequence-specific DNA binding transcription factor activity

Molecular Function

Transforming growth factor beta receptor, common-partner cytoplasmic mediator activity

Subcellular Location

Cytoplasm;Nucleus

Structure

Belongs to the dwarfin/SMAD family.

Alternative Nomenclature

(Small) Mothers Against Decapentaplegic antibody

Deleted in Pancreatic Carcinoma 4 antibody

DPC 4 antibody

hSMAD4 antibody

JIP antibody

MAD mothers against decapentaplegic homolog 4 antibody

MADH 4 antibody

Med antibody

Medea antibody

MYHRS antibody

Smad 4 antibody

General Information

Product

Phospho inaD Antibody Affinity Purified

Description

Affinity Purified Phospho Inactivation no afterpotential D, isoform A Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 600-700 on iNAD protein.

Accession #

NCBI:  NP_726260.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500

Protein

Uniprot #

Q24008

Overview

Utilizes a calcium-mediated process to function in the negative feedback regulation of the light activated signalling cascade in photoreceptors.

Molecular Function

Calmodulin binding, myosin binding, photoreceptor activity

Subcellular Location

Cell membrane, Peripheral membrane protein

Expression

Expression is detected in rhabdomers of the compound eyes and ocelli

Structure

Interacts with the C-terminus of trp.  Forms the inaD signalling complex through its interaction  with norpA and inaC.

Alternative Nomenclature

Inactivation-no-after-potential D protein antibody

inaD antibody

INAD_DROME antibody

General Information

Product

Phospho trp Antibody Affinity Purified

Description

Affinity Purified Phosphorylated Transient Receptor Potential isoform A Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Phosphorylated synthetic peptide corresponding to unique amino acid sequences on D. melanogaster Transient Receptor Potential isoform A protein.

Accession #

NCBI:  NP_476768.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:10,000

Protein

Uniprot #

P19334

Overview

A light-sensitive calcium channel that is required for inositide-mediated Ca2+ entry in the retina during phospholipase C (PLC)-mediated phototransduction. Ca2+ influx may then feed back and inhibit PLC, thereby facilitating phosphatidylinositol 4,5 bisphosphate (PIP2) recycling.

Molecular Function

Calcium channel, Ion channel, Calmodulin activity

Subcellular Location

Cell membrane; Multi-pass membrane protein

Expression

Expressed predominantly in the rhabdomeres of photoreceptor cells. Expressed in the third antennal segment and in the olfactory segment at approximately 70 hours after puparium formation during antennal development.

Structure

The C-terminus interacts with a PDZ domain of inaD to form the core of the inaD signaling complex. Other members of the complex include norpA (PLC), inaC (PKC), and possibly trpl, ninaC, FKBP59, calmodulin and rhodopsin. Forms homomultimers and heteromult

Alternative Nomenclature

DmTRP antibody

dTRP antibody

Transient receptor potential antibody

General Information

Product

Phospho Protein Vestigial Antibody Affinity Purified

Description

Affinity Purified Vestigial Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Phosphorylated synthetic peptide corresponding to unique amino acid sequences on D. melanogaster Vestigial protein.

Accession #

NCBI:  NP_523723.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q26366

Overview

Involved in determining which thoracic imaginal disk cells will form wings and halteres, perhaps by interacting with other nuclear regulatory proteins. When in combination with scalloped (sd), it acts as a transcriptional activation complex that regulates gene expression in the wing. Binding to sd switches the DNA target selectivity of sd. Required and sufficient for cell proliferation at the dorsal/ventral (D/V) boundary of the wing imaginal disk. Also required for cell proliferation in the wing imaginal disk, mediated via activation of E2f. By interacting with Dhfr, may control genes involved in DNA replication.

Molecular Function

DNA binding; Protein C-terminus binding

Subcellular Location

Nucleus

Expression

Expressed in the developing wing primordia initially along the D/V wing boundary, and by the late third larval instar, maximal expression is seen in cells at the D/V wing disk boundary. Less expression is seen in cells located farther from this boundary.

Alternative Nomenclature

VG

VSTGL

VG21

DMEL/CG3830

CCG3830