General Information

Product

Aquaporin 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Sheep, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 200-250 on human Aquaporin 2 protein.

Accession #

GenBank: CAG46821.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.62 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P34080

Overview

Forms a water-specific channel that provies the plasma mebranes of red cells and kidney proximal tubules with water permeability.

Molecular Function

Transport

Subcellular Location

Apical cell membrane; Multiplass membrane protein; Basolateral cell membrane

Expression

Renal collecting tubule

Structure

Contain two tandem repeats each with three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala

Alternative Nomenclature

ADH water channel antibody

AQP 2 antibody

AQP CD antibody

Aquaporin 2 collecting duct antibody

Aquaporin CD antibody

Collecting duct water channel protein antibody

MGC34501 antibody

Water channel aquaporin 2 antibody

Water channel protein for renal collecting duct antibody

WCH CD antibody

General Information

Product

Aquaporin 3 Antibody

Verified Applications

ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Sheep, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 200-250 on rat Aquaporin 3 protein.

Accession #

GenBank: CAG46822.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.57 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P47862

Overview

Promotes glycerol permeability and water transport across cell membranes.  Plays important role in regulating stratum corneum and epidermal glyceral content.  Involved in skin hydration, wound healing and tumorigenesis.

Molecular Function

Transport

Subcellular Location

Membrane; Multi-pass protein

Expression

Renal medulla and colon.

Structure

Contain two tandem repeats each with three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala

Alternative Nomenclature

AQP 3 antibody

Aquaglyceroporin-3 antibody

Aquaporin 3 (GIL blood group) antibody

Aquaporin-3 antibody

GIL antibody

Gill blood group antibody

General Information

Product

Aquaporin 4 Antibody

Verified Applications

ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Sheep, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-323 on rat Aquaporin 4 protein.

Accession #

NCBI:  NP_001641

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.62 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P55087

Overview

Forms water specific channel. Osmoreceptor which regulates water balance and mediates water flow within CNS.

Molecular Function

Transport

Subcellular Location

Membrane; Multi-pass protein

Expression

Abundant in mature brain but only weakly detectable in eye, kidney, intestine, and lung.

Structure

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Alternative Nomenclature

AQP 4 antibody

Aquaporin type 4 antibody

Aquaporin-4 antibody

HMIWC2 antibody

Mercurial insensitive water channel antibody

MGC22454 antibody

MIWC antibody

WCH 4 antibody

General Information

Product

Aquaporin 5 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Sheep, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-299 on rat Aquaporin 5 protein.

Accession #

GenBank: AAH78904

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.52-0.65 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q6AYU6

Overview

Forms a water-specific channel and is osmoreceptor which regulates body water balance and mediates water flow ithin the CNS.

Molecular Function

Transport

Subcellular Location

Membrane; Multi-pass protein

Expression

Expressed predominantly in the ependymal cell lining the aqueductal system and over the space of the brain in contact with subarachnoid space.  Weakly detectable in eye, kidney, intestine and lung.

Structure

Contain two tandem repeats each with three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala

Alternative Nomenclature

AQP 5 antibody

Aquaporin 5 antibody

General Information

Product

Aquaporin 6 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Sheep, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 220-270 on rat Aquaporin 6 protein.

Accession #

NCBI:  NP_071517.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:400

Protein

Uniprot #

Q9WTY0

Overview

Forms water specific channel.  Invovled in several physiological functions including glomerular filtration, tubular endocytosis, and acid-base metabolism

Molecular Function

Transport

Subcellular Location

Cytoplasmic vessicle membrane; Multi-pass membrane protein

Expression

Kidney

Structure

Contain two tandem repeats each with three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala

Alternative Nomenclature

AQP-6 antibody

AQP2L antibody

Aquaporin 2 like antibody

aquaporin 2 like kidney specific antibody

Aquaporin 6 antibody

Aquaporin 6 kidney specific antibody

hKID antibody

Human kidney water channel (hKID) mRNA, complete cds antibody

KID antibody

Kidney-specific aquaporin antibody

General Information

Product

Aquaporin 7 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Sheep, Mouse, Rat

Immunogen

Synthetic amidated peptide taken within amino acid region 150-200 on rat Aquaporin 7 protein, covalently modified and conjugated to KLH.

Accession #

NCBI:  NP_062030.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.58 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500; Predicted Molecular Weight: 37 kDa

Protein

Uniprot #

O54794

Overview

Forms water and glycerol channel

Molecular Function

Transport

Subcellular Location

Membrane; Multi-pass protein

Expression

Membranes

Structure

Contain two tandem repeats each with three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala

Alternative Nomenclature

AQP 7 antibody

AQP 7L antibody

AQP 9 antibody

AQPap antibody

Aquaglyceroporin-7 antibody

Aquaporin 7 like antibody

Aquaporin adipose antibody

Aquaporin-7 antibody

MGC149555 antibody

MGC149556 antibody

General Information

Product

Aquaporin 8 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Sheep, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 215-263 on rat Aquaporin 8 protein.

Accession #

GenBank: BAA21918

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.95 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P56405

Overview

Forms water specific channel.  Mercury-sensitive.  Plays role in spermatogenesis, fertilization, and in secretion of pancreatic juice and saliva.

Molecular Function

Transport

Subcellular Location

Membrane; Multi-pass protein

Expression

Sperm, pancreas and liver.  Less expressed in salivary gland and absoptive colonic epithelial cells.

Structure

Contain two tandem repeats each with three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala

Alternative Nomenclature

AQP8 antibody

Aquaporin 8 antibody

General Information

Product

Aquaporin 9 Antibody

Verified Applications

ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Sheep, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-294 on rat Aquaporin 9 protein.

Accession #

NCBI:  NP_075249

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.8 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P56627

Overview

Forms a channel with broad specificity.  Mediates passage of wide variety of non-charged solutes.

Molecular Function

Transport

Expression

Highly expressed in peripheral leukocytes. Also expressed in liver, lung, and spleen.

Subcellular Location

Membrane; Multi-pass protein

Structure

Contain two tandem repeats each with three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala

Alternative Nomenclature

AQP 9 antibody

Aquaglyceroporin-9 antibody

Aquaporin 9 antibody

HsT 17287 antibody

Small solute channel 1 antibody

SSC 1 antibody

General Information

Product

ARF1 Antibody

Description

Affinity Purified ADP-ribosylation factor 1 Antibody

Verified Applications

ELISA, ICC/IF, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on Rat ARF1 protein. The peptide was covalently modified post-synthetically to achieve desied antigenicity.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500. Predicted Molecular Weight 21 kDa

Protein

Uniprot #

P84079

Overview

GTP-binding protein that functions as an allosteric activator of cholera toxin catalytic subunt.

Molecular Function

ADP-ribosyltransferase; GTP binding; ER-Golgi transport

Subcellular Location

Gogli apparatus

Expression

Cell junction; Membrane; Synapse

Structure

Belongs to the small GTPase superfamily, Arf family

Alternative Nomenclature

DJ133P16.1 antibody

General Information

Product

ARF4 Antibody

Description

Affinity Purified ADP-ribosylation factor 4 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on ARF4 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.71-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P61751

Overview

GTP-binding protein that functions as an allosteric activator of cholera toxin catalytic subunt.

Molecular Function

ADP-ribosyltransferase; GTP binding; ER-Golgi transport

Subcellular Location

Gogli apparatus

Expression

Cell junction; Membrane; Synapse

Structure

Belongs to the small GTPase superfamily, Arf family

Alternative Nomenclature

ADP ribosylation factor 2 antibody

ADP ribosylation factor 4 antibody