General Information
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Product
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Shade Antibody BIOTIN-Conjugated
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Description
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BIOTIN-Conjugated Shade, isoform B Antibody C-epitope
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Verified Applications
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CM, ELISA, ICC, IHC, IF, WB
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Host
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Rabbit
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Species Cross Reactivity
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D. melanogaster
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Immunogen
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Synthetic peptide taken within amino acid region 160-230 on Spook protein.
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Accession #
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NCBI: NP_996074.1
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.55-0.68 µg/µl in antibody stabilization buffer
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Form
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BIOTIN-Conjugated
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Clonality
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Polyclonal
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Determinant
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C-epitope
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Storage
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-20⁰C for long term storage
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Application Protocol
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Confocal Microscopy
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1:200
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DB
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1:10,000
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ELISA
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1:10,000
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Immunocytochemistry
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1:200
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Immunofluorescence
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1:200
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Immunohistochemistry
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1:200
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Western Blot
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1:500
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Protein
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Uniprot #
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Q9VUF8
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Overview
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Required for CNS development; midline glial cells. Involved in the metabolism of insect hormones; responsible for all ecdysone 20-monooxygenase activity during embryonic, larval and adult stages. May be involved in the breakdown of synthetic insecticides.
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Molecular Function
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Monooxygenase, Oxidoreductase
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Subcellular Location
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Mitochondrion membrane
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Expression
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Strong expression by embryonic stage 10 in epidermis, decreases significantly in older embryos. Third instar larvae show expression in the midgut copper cells, Malpighian tubules and fat body. In the adult ovaries, expression is seen in both nurse cells a
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Structure
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Belongs to the cytochrome P450 family.
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Alternative Nomenclature
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SH2 domain-containing adapter protein D antibody
SHD antibody
Src homology 2 domain containing transforming protein D antibody Ecdysone 20-monooxygenase
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