General Information

Product

PAX2 isoform d + e Antibody

Description

Affinity Purified Paired box protein Pax-2 isoform d and Pax-2 isoform e Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 367-417 on human PAX2 isoform d protein.

Specificity

This antibody will detect Pax-2 isoform d and Pax-2 isoform e. Will not detect other Pax-2 isoforms.

Accession #

NCBI:  NP_003980.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-0.95 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q02962

Overview

Probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS.

Molecular Function

Developmental protein, core promoter proximal region sequence-specific DNA binding, DNA binding

Subcellular Location

Nucleus

Expression

Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system.

Structure

Mainly in fetal kidney and juvenile nephrogenic rests.

Alternative Nomenclature

Paired box 2 antibody

Paired box gene 2 antibody

paired box protein 2 antibody

Paired box protein Pax 2 antibody

Pax 2 antibody

General Information

Product

CD146 Antibody

Description

Affinity Purified Cell Surface Glycoprotein MUC18 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Rat

Immunogen

Synthetic peptide taken within amino acid region 330-353 on rat Cell Surface Glycoprotein MUC18 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q9EPF2

Overview

lays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Detected in lung, uterus and placenta (at protein level). Detected in heart, lung, kidney, adrenal gland, intestine, testis, skeletal muscle and aorta. Detected at low levels in adult brain, in particular in brain stem and spinal cord, but also in hippocampus, olfactory bulb and striatum (at protein level).

Structure

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Alternative Nomenclature

A32 antigen antibody

CD 146 antibody

Cell surface glycoprotein MUC18 antibody

Cell surface glycoprotein P1H12 antibody

Gicerin antibody

Mcam antibody

Melanoma adhesion molecule antibody

Melanoma associated antigen A32 antibody

Melanoma associated antigen MUC18 antibody

Melanoma associated glycoprotein MUC18 antibody

Melanoma cell adhesion molecule antibody

Melanoma-associated antigen A32 antibody

Melanoma-associated antigen MUC18 antibody

MelCAM antibody

MUC 18 antibody

S endo 1 antibody

S endo 1 endothelial associated antigen antibody

General Information

Product

BMP2 Antibody

Description

Affinity Purified Bone morphogenetic Protein 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 340-396 on human Bone morphogenetic Protein 2.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P12643

Overview

Induces cartilage and bone formation (PubMed:3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed:24362451).

Molecular Function

BMP receptor binding

co-receptor binding

cytokine activity

growth factor activity

phosphatase activator activity

retinol dehydrogenase activity

signaling receptor binding

SMAD binding

Subcellular Location

Secreted

Expression

Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.

Structure

Belongs to the TGF-beta family.

Alternative Nomenclature

BDA2 antibod

BMP 2 antibody

BMP 2A antibody

BMP-2 antibody

BMP-2A antibody

Bmp2 antibody

BMP2_HUMAN antibody

BMP2A antibody

Bone morphogenetic protein 2 antibody

Bone morphogenetic protein 2A antibody