General Information

Product

GFAP Antibody

Description

Affinity Purified Glial fibrillary acidic protein Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 50-100 on human glial fibrillary acidic protein.

Accession #

PRF:  226236

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P14136

Overview

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Molecular Function

Structural Constituent of Cytoskeleton; Astrocyte development; Bergmann glial cell differentiation

Subcellular Location

Cytoplasm

Expression

Expressed in cells lacking fibronectin.

Structure

Interacts with SYNM (By similarity). Isoform 3 interacts with PSEN1 (via N-terminus).

Alternative Nomenclature

Astrocyte antibody

FLJ42474 antibody

FLJ45472 antibody

GFAP antibody

Glial Fibrillary Acidic Protein antibody

Intermediate filament protein antibody

General Information

Product

Integrin beta 3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human Integrin Beta 3 subunit protein.

Accession #

GenBank: AAB23689.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P05106

Overview

Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Molecular Function

Cell adhesion, Host-virus interaction

Subcellular Location

Cell junction, Cell membrane, Cell projection, Membrane

Expression

Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.

Structure

Heterodimer of an alpha and a beta subunit. Beta-3 (ITGB3) associates with either alpha-IIb (ITGA2B) or alpha-V (ITGAV). Isoform Beta-3C interacts with FLNB. Interacts with COMP. Interacts with PDIA6 following platelet stimulation. Interacts with SYK; upon activation by ITGB3 promotes platelet adhesion. Interacts with MYO10. Interacts with DAB3. Interacts with FERMT2. Interacts with EMP2; regulates the levels of the heterodimer ITGA5:ITGB3 integrin expression on the plasma membrane (PubMed:16216233). Integrin ITGAV:ITGB3 interacts with FBLN5 (via N-terminus). Integrin ITGAV:ITGB3 interacts with herpes virus 8/HHV-8 glycoprotein B and acts as a receptor for the virus. Interacts with HIV-1 Tat.

Alternative Nomenclature

BDPLT16 antibody

BDPLT2 antibody

CD61 antibody

GP3A antibody

GT antibody

HPA 1 antibody

HPA 4 antibody

Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody

Integrin beta chain beta 3 antibody

ITGB 3 antibody

NAIT antibody

Platelet fibrinogen receptor, beta subunit antibody

Platelet glycoprotein IIIa antibody

Platelet membrane glycoprotein IIIa antibody

PTP antibody

General Information

Product

Shibire Antibody

Description

Affinity Purified Shibire, isoform K Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster (fruit fly)

Immunogen

Synthetic peptide taken within amino acid region 800-850 on D. melanogaster Shibire protein.

Accession #

NCBI:  NP_727910.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P27619

Overview

Microtubule-associated force-producing protein which is involved in the production of microtubule bundles and which is able to bind and hydrolyze GTP. Implicated in endocytic protein sorting.

Molecular Function

Hydrolase, Motor protein

Subcellular Location

Cytoplasm; Cytoskeleton

Structure

Contains 1 Dynamin-type G domain; Contains 1 GED domain; Contains 1 PH domain

Alternative Nomenclature

anon-WOO153538

CG18102

Ddyn

Ddyn3

Ddyn4

Shi

Shibere

General Information

Product

CD99 Antibody

Description

Affinity Purified CD99 Antigen Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Baboon, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 50-100 to human CD99 antigen.

Accession #

GenBank: EAW98700.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

UPI0000EE42C0

Overview

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes.

Molecular Function

Cell Adhesion

Subcellular Location

Membrane

Structure

Belongs to CD99 family

Alternative Nomenclature

CD99 antibody

CD99 antigen antibody

CD99 molecule antibody

CD99X antibody

E2 antigen antibody

Ewing Sarcoma antibody

MIC 2X antibody

MIC 2Y antibody

MIC2 antibody

MSK5X antibody

Protein MIC2 antibody

Surface antigen MIC2 antibody

T cell surface glycoprotein E2 antibody

General Information

Product

CD99 Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 97-147 to human CD99 antigen.

Accession #

GenBank: EAW98700.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

UPI0000EE42C0

Overview

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes.

Molecular Function

Cell Adhesion

Subcellular Location

Membrane

Structure

Belongs to CD99 family

Alternative Nomenclature

12E7 antibody

CD99 antibody

CD99 antigen antibody

CD99 molecule antibody

CD99X antibody

E2 antigen antibody

Ewing Sarcoma antibody

MIC 2X antibody

MIC 2Y antibody

MIC2 antibody

MSK5X antibody

Protein MIC2 antibody

Surface antigen MIC2 antibody

T cell surface glycoprotein E2 antibody

General Information

Product

Calponin Antibody

Description

Affinity Purified Calponin 1, basic, smooth muscle Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 120-170 on human Calponin 1, basic, smooth muscle protein.

Accession #

NCBI:  XP_005259798.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

B7Z7E1

Overview

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

Molecular Function

Actin-Binding; actomyosin structure organization

Subcellular Location

Cytoskeleton; Focal Adhesion

Expression

Smooth muscle, and tissues containing significant amounts of smooth muscle.

Structure

Belongs to the calponin family.UniRule annotation

Contains 1 CH (calponin-homology) domain.

Contains 3 calponin-like repeats.

Alternative Nomenclature

Basic calponin antibody

Calponin 1 antibody

Calponin 1 basic smooth muscle antibody

Calponin H1 antibody

Calponin H1 smooth muscle antibody

Calponins basic antibody

CNN 1 antibody

Sm Calp antibody

SMCC antibody

smooth muscle antibody

General Information

Product

Calponin Antibody

Description

Affinity Purified Calponin 1, basic, smooth muscle Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 200-247 on human calponin 1, basic, smooth muscle protein.

Accession #

NCBI:  XP_005259798.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

B7Z7E1

Overview

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

Molecular Function

Actin-Binding; actomyosin structure organization

Subcellular Location

Cytoskeleton; Focal Adhesion

Expression

Smooth muscle, and tissues containing significant amounts of smooth muscle.

Structure

Belongs to the calponin family.UniRule annotation

Contains 1 CH (calponin-homology) domain.

Contains 3 calponin-like repeats.

Alternative Nomenclature

Basic calponin antibody

Calponin 1 antibody

Calponin 1 basic smooth muscle antibody

Calponin H1 antibody

Calponin H1 smooth muscle antibody

Calponins basic antibody

CNN 1 antibody

Sm Calp antibody

SMCC antibody

smooth muscle antibody

General Information

Product

MAPK2 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-350 on human Mitogen-activated protein kinase 2.

Accession #

NCBI:  NP_116584

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P49137

Overview

Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, ELAVL1, HNRNPA0, HSF1, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Nucleus

Expression

Expressed in all tissues examined.

Structure

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Alternative Nomenclature

ERK 2 antibody

ERT1 antibody
Extracellular Signal Regulated Kinase 2 antibody

MAP kinase 2 antibody

MAPK 2 antibody
Mitogen-activated protein kinase 2 antibody
protein kinase, mitogen-activated, 2 antibody
protein tyrosine kinase ERK2 antibody

General Information

Product

Occludin Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 450-523 on rat Occludin protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q6P6T5

Overview

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. May be involved in the organization of actin in endothelial cells.

Molecular Function

protein domain specific binding

Subcellular Location

Cell membrane

Structure

he C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction.

Alternative Nomenclature

BLCPMG antibody

Occludin antibody

Ocln antibody

OCLN_HUMAN antibody

Phosphatase 1 regulatory subunit 115 antibody

PPP1R115 antibody

PTORCH1 antibody

Tight junction protein occludin antibody

General Information

Product

CD146 Antibody

Description

Affinity Purified Cell Surface Glycoprotein MUC18 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Rat

Immunogen

Synthetic peptide taken within amino acid region 330-353 on rat Cell Surface Glycoprotein MUC18 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q9EPF2

Overview

lays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Detected in lung, uterus and placenta (at protein level). Detected in heart, lung, kidney, adrenal gland, intestine, testis, skeletal muscle and aorta. Detected at low levels in adult brain, in particular in brain stem and spinal cord, but also in hippocampus, olfactory bulb and striatum (at protein level).

Structure

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Alternative Nomenclature

A32 antigen antibody

CD 146 antibody

Cell surface glycoprotein MUC18 antibody

Cell surface glycoprotein P1H12 antibody

Gicerin antibody

Mcam antibody

Melanoma adhesion molecule antibody

Melanoma associated antigen A32 antibody

Melanoma associated antigen MUC18 antibody

Melanoma associated glycoprotein MUC18 antibody

Melanoma cell adhesion molecule antibody

Melanoma-associated antigen A32 antibody

Melanoma-associated antigen MUC18 antibody

MelCAM antibody

MUC 18 antibody

S endo 1 antibody

S endo 1 endothelial associated antigen antibody