General Information

Product

Bestrophin Antibody

Description

Affinity Purified Bestrophin 1 Antibody C-epitope

Verified Applications

CM, ELISA, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 500-585 on human Bestrophin-1 isoform 1 protein.

Accession #

NCBI: NP_004174.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DOT Blot

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O76090

Overview

Forms calcium sensitive chloride channels. Highly permeable to bicarbonate.

Molecular Function

Ion transport; Sensory transduction; Transport; Vision

Subcellular Location

Cell membrane; Multi-pass membrane protein; Basolateral cell membrane

Expression

Predominantly expressed in the basolateral membrane of the retinal pigment epithelium.

Structure

Tetramer or pentamers

Alternative Nomenclature

ARB antibody

BEST 1 antibody

BEST antibody

Best disease antibody

BEST1 antibody

Bestrophin1 antibody

BMD antibody

mBest1 antibody

RP50 antibody

TU15B antibody

Vitelliform macular dystrophy 2 antibody

VMD2 antibody

General Information

Product

PODXL Antibody

Description

Affinity Purified Pan Podocalyxin Antibody

Verified Applications

DB, ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

C-epitope synthetic peptide taken within amino acid region 510-560 on human PODXL protein.

Accession #

NCBI:  NP_001018121.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O00592

Overview

Involved in the regulation of both adhesion and cell morphology and cancer progression.  Funciton as an anti-aging adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion.

Subcellular Location

Apical cell membrane, Cell projection, Membrane

Expression

Glomerular epithelium cell (podocyte)

Structure

Monomer; when associated with the membrane raft.  Oligomer; when integrated in the apical membrane.

Alternative Nomenclature

GCTM-2 antigen antibody

Gp200 antibody

MGC138240 antibody

PC antibody

PCLP antibody

PCLP1 antibody

Pcx antibody

Podocalyxin antibody

Podocalyxin-like protein 1 antibody

Podxl antibody

General Information

Product

PODXL1 Antibody

Description

Affinity Purified Podocalyxin isoform 1 Antibody N-epitope

Verified Applications

DB, ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide corresponding amino acid region 200-250 on human PODXL protein.

Accession #

NCBI:  NP_001018121.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O00592

Overview

Involved in the regulation of both adhesion and cell morphology and cancer progression.  Funciton as an anti-aging adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion.

Subcellular Location

Apical cell membrane, Cell projection, Membrane

Expression

Glomerular epithelium cell (podocyte)

Structure

Monomer; when associated with the membrane raft.  Oligomer; when integrated in the apical membrane.

Alternative Nomenclature

GCTM-2 antigen antibody

Gp200 antibody

MGC138240 antibody

PC antibody

PCLP antibody

PCLP1 antibody

Pcx antibody

Podocalyxin antibody

Podocalyxin-like protein 1 antibody

Podxl antibody

General Information

Product

Syndecan-1 Antibody Affinity Purified

Description

Affinity Purified Syndecan 1 Antibody C-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

C-epitope synthetic peptide taken within amino acid region 250-350 on human Syndecan-1 protein.

Accession #

GenBank: AAH08765.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.61 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P18827

Overview

Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.

Molecular Function

Protein C-terminus binding

Subcellular Location

Membrane; Single-pass type I membrane protein

Structure

Interacts with CDCP1. Interacts (via C-terminus) with TIAM1 (via PDZ domain).

Alternative Nomenclature

CD 138 antibody

Heparan sulfate proteoglycan fibroblast growth factor receptor antibody

SDC 1 antibody

SDC antibody

syndecan proteoglycan 1 antibody

General Information

Product

Syndecan-1 Antibody Affinity Purified

Description

Affinity Purified Syndecan 1 Antibody N-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

N-epitope synthetic peptide taken within amino acid region 1-100 on human Syndecan-1 protein.

Accession #

GenBank: AAH08765.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.61 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P18827

Overview

Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.

Molecular Function

Protein C-terminus binding

Subcellular Location

Membrane; Single-pass type I membrane protein

Structure

Interacts with CDCP1. Interacts (via C-terminus) with TIAM1 (via PDZ domain).

Alternative Nomenclature

CD 138 antibody

Heparan sulfate proteoglycan fibroblast growth factor receptor antibody

SDC 1 antibody

SDC antibody

syndecan proteoglycan 1 antibody

General Information

Product

FAK1 Antibody

Description

Affinity Purified Focal Adhesion Kinase 1

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 740-790 on human focal adhesion kinase 1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q05397

Overview

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.

Molecular Function

Developmental protein; Kinase; Transferase; Tyrosine-protein kinase

Subcellular Location

Cell junction › focal adhesion

Cell membrane; Peripheral membrane protein; Cytoplasmic side

Cytoplasm › cell cortex

Cytoplasm › cytoskeleton

Cytoplasm › cytoskeleton › microtubule organizing center › centrosome

Nucleus

Expression

Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous.

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.

Contains 1 FERM domain.

Contains 1 protein kinase domain.

Alternative Nomenclature

FADK 1 antibody

FADK antibody

FAK 1 antibody

FAK related non kinase polypeptide antibody

FAK1 antibody

Focal adhesion kinase 1 antibody

Focal adhesion kinase isoform FAK Del33 antibody

Focal adhesion kinase related nonkinase antibody

FRNK antibody

p125FAK antibody

pp125FAK antibody

PPP1R71 antibody

Protein phosphatase 1 regulatory subunit 71 antibody

Protein tyrosine kinase 2 antibody

Protein Tyrosine Kinase Cytoplasmic antibody

PTK 2 antibody

PTK2 protein tyrosine kinase 2 antibody

General Information

Product

CD56 / NCAM Antibody

Description

Affinity Purified Neural Cell Adhesion Molecule 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 800-847 on human neural cell adhesion molecule 1.

Accession #

GenBank: EAW67208.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

UPI0000EE39A0

Overview

Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. Plays a role in neuronal positioning of pyramidal neurons and in regulation of both the number of interneurons and the efficacy of GABAergic synapses. May play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins. Recruits ANK3 to the plasma membrane.

Molecular Function

Developmental protein; Cell adhesion; Differentiation; Neurogenesis

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Expressed in the fetal and adult brain as well as in Schwann cell culture. Also detected in adult peripheral tissues.

Alternative Nomenclature

antigen MSK39 identified by monoclonal antibody 5.1H11 antibody

antigen recognized by monoclonal antibody 5.1H11 antibody

CD 56 antibody

CD56 antigen antibody

Cell Adhesion Molecule Neural 1 antibody

MSK39 antibody

N CAM 180 antibody

N-CAM-1 antibody

NCAM 120 antibody

NCAM 140 antibody

NCAM antibody

NCAMC antibody

NCAM1 antibody

Neural cell adhesion molecule 1 antibody

Neural cell adhesion molecule NCAM antibody

General Information

Product

CD47 Antibody

Description

Affinity Purified Leukocyte surface antigen CD47 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Predicted: Rat

Immunogen

Synthetic peptide taken within amino acid region 100-150 on human CD47 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q08722 

Overview

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

Molecular Function

Thrombospondin receptor activity; Cell adhesion

Subcellular Location

Cell membrane, Membrane

Expression

Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.

Structure

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Alternative Nomenclature

Antigen identified by monoclonal antibody 1D8 antibody

Antigenic surface determinant protein OA3 antibody

CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antibody

CD47 glycoprotein antibody

CD47 molecule antibody

IAP antibody

Integrin Associated Protein antibody

Integrin associated signal transducer antibody

Leukocyte surface antigen CD47 antibody

MER6 antibody

OA3 antibody

Protein MER6 antibody

Rh related antigen antibody

General Information

Product

Phospho-Doublecortin Antibody

Description

Affinity Purified Phosphorylated Neuronal migration protein doublecortin Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 200-250 on human Mesencephalic astrocyte-derived neurotrophic factor.

Accession #

NCBI:  NP_835365

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Modification

Phosphorylated

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O43602

Overview

Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.

Molecular Function

Developmental protein

Subcellular Location

Cytoplasm, Cell projection

Expression

Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.

Structure

Contains 2 doublecortin domains.

Alternative Nomenclature

DBCN antibody
Dbct antibody
DC antibody
DCX antibody
Doublecortex antibody
Doublin antibody
Lis X antibody
Lissencephalin X antibody
Lissencephaly X linked doublecortin antibody
LISX antibody
Neuronal migration protein doublecortin antibody
SCLH antibody
XLIS antibody

General Information

Product

MAPK2 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-350 on human Mitogen-activated protein kinase 2.

Accession #

NCBI:  NP_116584

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P49137

Overview

Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, ELAVL1, HNRNPA0, HSF1, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Nucleus

Expression

Expressed in all tissues examined.

Structure

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Alternative Nomenclature

ERK 2 antibody

ERT1 antibody
Extracellular Signal Regulated Kinase 2 antibody

MAP kinase 2 antibody

MAPK 2 antibody
Mitogen-activated protein kinase 2 antibody
protein kinase, mitogen-activated, 2 antibody
protein tyrosine kinase ERK2 antibody