General Information

Product

SERHL Antibody Antigenic Blocking Peptide

Description

Antigenic Blocking Peptide Serine hydrolase-like protein 2 isoform a Antibody Mid-Region

Verified Applications

Immunodepletion, Immunocompetition

Immunogen

Synthetic peptide

Accession #

NCBI:  NP_055324.2

Physical Properties

Quantity

250 µg

Volume

100 µl

Form

Antigenic Blocking Peptide

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

Western Blot

1:500

Protein

Uniprot #

Q9H4I8

Overview

Probable serine hydrolase. May be related to cell muscle hypertrophy.

Molecular Function

Hydrolase activity

Subcellular Location

Cytoplasm, perinuclear region; peroxisome. (Note: Concentrated in perinuclear vesicles. May be located in peroxisomes)

Alternative Nomenclature

BK126B4.1 antibody

BK126B4.2 antibody

HS126B42 antibody 

serine hyrdolase 1 antibody

General Information

Product

SERHL Antibody Antigenic Blocking Peptide

Description

Antigenic Blocking Peptide Serine hydrolase-like protein 2 isoform a Antibody N-epitope

Verified Applications

Immunodepletion, Immunocompetition

Immunogen

Synthetic peptide

Accession #

NCBI:  NP_055324.2

Physical Properties

Quantity

250 µg

Volume

100 µl

Form

Antigenic Blocking Peptide

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Western Blot

1:500

Protein

Uniprot #

Q9H4I8

Overview

Probable serine hydrolase. May be related to cell muscle hypertrophy.

Molecular Function

Hydrolase activity

Subcellular Location

Cytoplasm, perinuclear region; peroxisome. (Note: Concentrated in perinuclear vesicles. May be located in peroxisomes)

Alternative Nomenclature

BK126B4.1 antibody

BK126B4.2 antibody

HS126B42 antibody 

serine hyrdolase 1 antibody

General Information

Product

TGM1 Antibody

Description

Affinity Purified Coagulation factor XIII A chain Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IHC, IF, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on Coagulation factor XIIIA chain precursor

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.61-0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

Dot Blot

1:30,000

ELISA

1:30,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Western Blot

1:450

Protein

Uniprot #

Q8BH61

Overview

Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.

Molecular Function

metal ion binding, protein-glutamine gamma-glutamyltransferase activity

Subcellular Location

Cytoplasm, Secreted

Structure

Tetramer of two A chains and two B chains

Alternative Nomenclature

ARCI1 antibody

Epidermal TGase antibody

ICR2 antibody

KTG antibody

LI antibody

LI1 antibody

Protein glutamine gamma glutamyltransferase K antibody

TG(K) antibody

TGase 1 antibody

TGASE antibody

TGase K antibody

TGK antibody

TGM1 antibody

Transglutaminase 1 (K polypeptide epidermal type I, protein glutamine gamma glutamyltransferase) antibody

Transglutaminase 1 antibody

Transglutaminase K antibody

General Information

Product

TGM1 Antibody Affinity Purified

Description

Affinity Purified Coagulation factor XIII A chain Antibody N-epitope

Verified Applications

CM, ELISA, ICC, IHC, IF, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on Coagulation factor XIIIA chain precursor

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.61-0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:30,000

ELISA

1:30,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Western Blot

1:450

Protein

Uniprot #

Q8BH61

Overview

Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin (By similarity).

Molecular Function

metal ion binding, protein-glutamine gamma-glutamyltransferase activity

Subcellular Location

Cytoplasm, Secreted

Structure

Tetramer of two A chains and two B chains

Alternative Nomenclature

ARCI1 antibody

Epidermal TGase antibody

ICR2 antibody

KTG antibody

LI antibody

LI1 antibody

Protein glutamine gamma glutamyltransferase K antibody

TG(K) antibody

TGase 1 antibody

TGASE antibody

TGase K antibody

TGK antibody

TGM1 antibody

Transglutaminase 1 (K polypeptide epidermal type I, protein glutamine gamma glutamyltransferase) antibody

Transglutaminase 1 antibody

Transglutaminase K antibody

General Information

Product

ATG16L1 Antibody

Description

Affinity Purified Autophagy-related protein 16-1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Predicted: Human

Immunogen

Synthetic peptide taken within amino acid region 560-610 on human Autophagy-related protein 16-1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q676U5

Overview

Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.

Molecular Function

Identical protein binding, Ubiquitin-like protein transferase activity

Subcellular Location

Cytoplasm, Preautophagosomal structure membrane, Peripheral membrane protein

Structure

Homooligomer. Interacts with WIPI2, FIP200 and ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Interacts with RAB33B. Interacts with TMEM

Alternative Nomenclature

APG16 like 1 antibody

APG16L antibody

APG16L beta antibody

ATG16 autophagy related 16 like 1 antibody

ATG16A antibody

ATG16L antibody

Autophagy related protein 16 1 antibody

IBD10 antibody

WD repeat domain 30 antibody

WDR30 antibody

General Information

Product

Legumain Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-150 on human Legumain protein.

Accession #

NCBI:  XP_016876954

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q99538

Overview

Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

Subcellular Location

Lysosome

Expression

Ubiquitous. Particularly abundant in kidney, heart and placenta.

Structure

In the zymogen form, the uncleaved propeptide blocks access to the active site.

Alternative Nomenclature

AEP antibody
Asparaginyl endopeptidase antibody
cysteine 1 antibody
Cysteine protease 1 antibody
Legumain antibody
LGMN antibody
LGMN1 antibody
Protease antibody
Protease cysteine 1 (legumain) antibody
Protease cysteine 1 antibody
PRSC1 antibody

General Information

Product

Von Hippel Lindau Antibody

Description

Affinity Purified Von Hippel-Lindau disease tumor suppressor Antibody

Verified Applications

ELISA, IHC, IP, WB

Immunogen

Synthetic peptide taken within amino acid region 130-200 on human Von Hippel-Lindau disease tumor suppressor protein.

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:150

Immunohistochemistry

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P40337

Overview

Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2.

Molecular Function

Enzyme binding, Transcription factor binding, Ubiquitin protein ligase activity,

Subcellular Location

Cytoplasm, Peripheral membrane protein, nucleus

Expression

Expressed in the adult and fetal brain and kidney.

Structure

Belongs to the VHL family.

Alternative Nomenclature

Elongin binding protein antibody

G7 protein antibody

HRCA 1 antibody

Protein G7 antibody

pVHL antibody

RCA 1 antibody

VHL 1 antibody

VHLH antibody

Von Hippel Lindau disease tumor suppressor antibody

von Hippel Lindau syndrome antibody

von Hippel Lindau tumor suppressor antibody

Von Hippel Lindau tumor suppressor, E3 ubiquitin protein ligase antibody

General Information

Product

VPS36 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 250-310 on human Vacuolar Protein-Sorting-associated protein 36 protein.

Accession #

NCBI:  NP_057159

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q86VN1

Overview

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3.

Molecular Function

phosphatidylinositol-3-phosphate binding

protein C-terminus binding

ubiquitin binding

Subcellular Location

Nucleus

Endosome

Late endosome

Expression

Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45. Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and two copies of VPS25 (PubMed:14519844). Interacts with VPS25, SNF8, TSG101 and CHMP6 (PubMed:14505570, PubMed:14519844, PubMed:16973552, PubMed:18539118). Interacts (via GLUE domain) with ubiquitin (PubMed:17057716). Interacts with RILPL1 (via the C-terminal domain); which recruits ESCRT-II to the endosome membranes (PubMed:17010938). Interacts with ECPAS (PubMed:20682791).

Structure

The GLUE domain (GRAM-like ubiquitin-binding in EAP45) mediates binding to ubiquitin and phosphoinosides.

Alternative Nomenclature

C13orf9 antibody

CGI 145 antibody

EAP45 antibody

ELL associated protein of 45 kDa antibody

ESCRT-II complex subunit VPS36 antibody

Vacuolar protein-sorting-associated protein 36 antibody

Vps36 antibody