Phospho-MLKL Blocking Peptide

General Information

Product

Phospho-MLKL Peptide

Description

Antigenic Blocking Peptide Phosphorylated Mixed lineage kinase domain-like protein MLKL Antibody

Verified Applications

Immunodepletion, Immunocompetition

Peptide Description

Synthetic peptide corresponding to amino acids within region 350-400 on human Mixed lineage kinase domain-like protein MLKL

Physical Properties

Quantity

250 µg

Volume

100 µl

Form

Antigenic Blocking Peptide

Modification

Phosphorylated

Storage

-20?C for long term storage

Application Protocol

Immunodepletion, Immunocompetition

Refer to competition assay protocol

Protein

Uniprot #

Q8NB16

Overview

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Does not have protein kinase activity (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671).

Molecular Function

ATP binding

identical protein binding

JUN kinase kinase kinase activity

protein-containing complex binding

protein kinase activity

protein kinase binding

Subcellular Location

Nucleus, Plasma membrane, cytoplasm

Structure

Homooligomer (PubMed:29883610). Homotrimer; forms homotrimers on necroptosis induction (PubMed:24316671). Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3 (PubMed:22265414).

Alternative Nomenclature

9130019I15Rik antibody

FLJ34389 antibody

hMLKL antibody

Mixed lineage kinase domain like antibody

Mixed lineage kinase domain like pseudokinase antibody

Mixed lineage kinase domain-like protein antibody

Mlkl antibody

MLKL_HUMAN antibody