General Information
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Product
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MYLK Antibody
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Description
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Affinity Purified Myosin light chain kinase, smooth muscle isoform 1 Antibody
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Verified Applications
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ELISA, IHC, IP, WB
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Host
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Chicken
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Species Cross Reactivity
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Human, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 1870-1907 on human Myosin light chain kinase, smooth muscle isoform 1 protein.
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.5 µg/µl in antibody stabilization buffer
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Immunoglobulin
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IgY
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Form
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Affinity Purified
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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ELISA
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1:10,000
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Immunohistochemistry |
1:50-1:100 |
Immunoprecipitation
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1:200
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Western Blot
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1:500
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Protein
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Uniprot #
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Q15746
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Overview
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Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma.
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Molecular Function
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Kinase, Serine/threonine-protein kinase, Transferase
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Subcellular Location
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Cytoplasm, Cell projection > lamellipodium, Cleavage furrow, Cytoplasm > cytoskeleton
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Expression
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Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues. Accumulates in well differentiated enterocytes of the intestinal epithelium in response to tumor necrosis factor (TNF).
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Structure
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All isoforms including Telokin bind calmodulin. Interacts with SVIL. Interacts with CTTN; this interaction is reduced during thrombin-induced endothelial cell (EC) contraction but is promoted by the barrier-protective agonist sphingosine 1-phosphate (S1P) within lamellipodia. A complex made of ABL1, CTTN and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Binds to NAA10/ARD1 and PTK2B/PYK2.
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Alternative Nomenclature
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caMLCK antibody
cardiac MLCK antibody
Cardiac-MyBP-C-associated Ca/CaM kinase antibody
MLC kinase antibody
MLCK antibody
MLCK2 antibody
Mylk3 antibody
Myosin light chain kinase 3 antibody
Putative myosin light chain kinase 3 antibody
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