General Information

Product

Axin 2 Antibody

Description

Affinity Purified Axis Inhibition Protein 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 800-843 on human Axin 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9Y2T1

Overview

Inhibitor of Wnt signaling pathway.  Down-regulates beta-catenin.

Molecular Function

Developmental Protein; Wnt signaling pathway

Subcellular Location

Cytoplasm

Expression

Brain and lymphoblast

Structure

Contains 1 DIX domain and 1 RGS domain

Alternative Nomenclature

Axil antibody

Axin like protein antibody

Axis inhibition protein 2 antibody

Conductin antibody

General Information

Product

HDAC4 Antibody

Description

Affinity Purified Histone deacetylase 4 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1,000-1,084 on human HDAC4 protein.

Accession #

GeneBank: AAH39904

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.63 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q86YH7

Overview

Responsible for deacetylation of lysine residues on the N-terminal part of the core histones.  Histone deacetylation gives a tag for epigenetic repression and plays and important role in transcriptional regulation, cell cycle progression and developmental events.

Molecular Function

Metal-binding; Zinc

Subcellular Location

Nucleus

Structure

Belongs to the histone deacetylase family. HD type 2 subfamily

Alternative Nomenclature

AHO3 antibody

BDMR antibody

EC 3.5.1.98 antibody

HA6116 antibody

HD 4 antibody

HDAC A antibody

Histone Deacetylase A antibody

KIAA0288 antibody

General Information

Product

SFRP2 Antibody 

Description

Affinity Purified Secreted frizzled-related protein 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 225-295 on human SFRP2.

Accession #

GenBank: AAH08666.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q96HF1

Overview

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.

Molecular Function

Endopeptidase activator activity, integrin binding, PDZ domain binding, Wnt-activated receptor activity, fibronectin binding, receptor agonist activity, Wnt-protein binding

Subcellular Location

Secreted

Expression

Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon.

Alternative Nomenclature

AI851596 antibody
Frizzled related protein 2 antibody
FRP2 antibody
SARP1 antibody
SDF5 antibody
Secreted apoptosis related protein 1 antibody
Secreted frizzled-related protein 2 antibody

General Information

Product

SFRP5 Antibody

Description

Affinity Purified Secreted frizzled-related protein 5 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-200 on SFRP5

Accession #

GenBank: AAD25052.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.67 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q5T4F7

Overview

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina.

Molecular Function

PDZ domain binding, Wnt-protein binding, Wnt-activated receptor activity

Subcellular Location

Secreted

Expression

Highly expressed in the retinal pigment epithelium (RPE) and pancreas. Weak expression in heart, liver and muscle.

Alternative Nomenclature

Frizzled related protein 1b

FRP 1b

SARP3

Secreted apoptosis related protein 3

Secreted frizzled related protein 5

General Information

Product

DISC1 Antibody

Description

Affinity Purified Disrupted in Schizophrenia 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 730-750 on DISC1 protein.

Accession #

GenBank:  CAI17205

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.72-0.76 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q5T409

Overview

Involved in the regulation of multiple aspects of embryonic and adult neurogenesis. Required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus.

Molecular Function

Neurogenesis, Wnt signaling pathway

Subcellular Location

Membrane, Microtubule, Postsynaptic cell membrane, Synapse

Expression

Ubiquitous.

Alternative Nomenclature

C1orf136 antibody

KIAA0457 antibody

RP4-730B13.1 antibody

SCZD9 antibody

General Information

Product

GPCR LGR6 Antibody Affinity Purified

Description

Affinity Purified Leucine-rich repeat-containing G protein-coupled receptor 6 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 850-920 on human LRG6 protein.

Accession #

Gen Bank: CAI15929.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9HBX8

Overview

Receptor for R-spondins that potentiates canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in epidermis.

Molecular Function

Transmembrane signaling receptor activity

Subcellular Location

Cell membrane, multi-pass membrane protein

Alternative Nomenclature

FLJ14471 antibody

Gonadotropin receptor antibody

GPCR antibody

Leucine-rich repeat-containing G protein-coupled receptor 6 antibody

Lgr6 antibody

VTS20631 antibody

General Information

Product

TRIF Antibody 

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on TRIF protein.

Accession #

GenBank:  BAA76376.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q9CQE0

Overview

E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway (By similarity).

Molecular Function

Ligase, Zinc ion binding

Subcellular Location

Nucleus

Structure

Interacts with NLK (via C-terminus).

Alternative Nomenclature

MGC35334 antibody

Proline rich vinculin and TIR domain containing protein B antibody

Proline-rich antibody

PRVTIRB antibody

Putative NF kappa B activating protein 502H antibody

Putative NFkB activating protein antibody

TICAM 1 antibody

TIR domain-containing adapter protein inducing IFN-beta antibody

Toll interleukin 1 receptor domain containing adapter protein inducing interferon beta antibody

Toll like receptor adaptor molecule 1 antibody

Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta antibody

TRIF antibodyvinculin and TIR domain-containing protein B antibody

General Information

Product

beta Catenin Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

N-epitope synthetic peptide taken within amino acid region 50-100 on human beta CTNNB protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P35222 

Overview

Major downstream component of the canonical Wnt signalling pathway.  Also involved in the regulation of cell adhesion and acts as a negative regulator of centrosome cohesion.

Molecular Function

Activator; Alpha-catenin binding; Cadherin binding; signal transducer activity

Subcellular Location

Cytoplasm; Nucleus; Cell junction; Cell Membrane

Expression

Expressed in hair follicle cell types including basal and peripheral matrix cells as well as cells of the outer and inner root sheaths.  Also expressed in colon and is present in cortical neurons at protein level.

Structure

Two separate complex associated pools found in cytoplasm, majority as compenent of an E-cadheren/catenin adhesion complex.  Another cytoplasmic pool is a part of a large complex which holds AXIN1, AXIN2, APC, CSNK1A1 and GSK3B

Alternative Nomenclature

Cadherin associated protein antibody

Catenin (cadherin associated protein), beta 1, 88kDa antibody

CATNB antibody

CHBCAT antibody

General Information

Product

beta Catenin Antibody Affinity Purified

Description

Affinity Purified Beta Catenin Antibody

C-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

C-epitope synthetic peptide taken within amino acid region 740-790 on human beta CTNNB protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P35222

Overview

Major downstream component of the canonical Wnt signalling pathway.  Also involved in the regulation of cell adhesion and acts as a negative regulator of centrosome cohesion.

Molecular Function

Activator; Alpha-catenin binding; Cadherin binding; signal transducer activity

Subcellular Location

Cytoplasm; Nucleus; Cell junction; Cell Membrane

Expression

Expressed in hair follicle cell types including basal and peripheral matrix cells as well as cells of the outer and inner root sheaths.  Also expressed in colon and is present in cortical neurons at protein level.

Structure

Two separate complex associated pools found in cytoplasm, majority as compenent of an E-cadheren/catenin adhesion complex.  Another cytoplasmic pool is a part of a large complex which holds AXIN1, AXIN2, APC, CSNK1A1 and GSK3B

Alternative Nomenclature

Cadherin associated protein antibody

Catenin (cadherin associated protein), beta 1, 88kDa antibody

CATNB antibody

CHBCAT antibody

General Information

Product

Sall4C Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 226-276 on human SALL4C protein.

Accession #

GenBank: AAO16567.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q6Y8G5

Overview

Transcription factor with a key role in the maintenance and self-renewal of embryonic and hematopoietic stem cells.

Molecular Function

DNA binding; Metal ion binding; Transcription Regulation

Subcellular Location

Cytoplasm, Nucleus

Structure

Belongs to the sal C2H2-type zinc-finger protein family.

Contains 7 C2H2-type zinc fingers.

Alternative Nomenclature

AA407717 antibody

AL022809 antibody

AW536104 antibody

C330011P20Rik antibody

C78083 antibody

C78563 antibody

DRRS antibody

HSAL4 antibody

Sal-like Protein 4 antibody

Spalt like transcription factor 4 antibody

Tex20 antibody

Zinc finger protein 797 antibody

Zinc finger protein SALL4 antibody

ZNF797 antibody