General Information |
|
Product |
B-raf Antibody BIOTIN-Conjugated |
Description |
BIOTIN-Conjugated Serine/threonine-protein kinase B-raf Antibody |
Verified Applications |
ELISA, IHC, IP, WB |
Host |
Rabbit |
Species Cross Reactivity |
Human, Mouse |
Immunogen |
Synthetic peptide taken within amino acid region 700-750 on human serine/threonine-protein kinase B-raf protein. |
Accession # |
NCBI: NP_004324.2 |
|
|
Quantity |
100 µg |
Volume |
200 µl |
Concentration |
0.5 µg/µl in antibody stabilization buffer |
Immunoglobulin |
IgG |
Form |
BIOTIN-Conjugated |
Clonality |
Polyclonal |
Storage |
-20⁰C for long term storage |
|
|
ELISA |
1:10,000 |
Immunohistochemistry | 1:50-1:100 |
Immunoprecipitation |
1:200 |
Western Blot |
1:500 |
|
|
Uniprot # |
|
Overview |
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. |
Molecular Function |
Kinase, Serine/threonine-protein kinase, Transferase |
Subcellular Location |
Nucleus, Cytoplasm, Cell membrane |
Expression |
Brain and testis. |
Structure |
Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-753. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1. Interacts with DGKH. Interacts with PRMT5. Interacts with KSR2. |
Alternative Nomenclature |
94 kDa B raf protein antibody B raf 1 antibody B Raf proto oncogene serine threonine protein kinase antibody B RAF1 antibody B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody BRAF 1 antibody cRmil antibody Murine sarcoma viral v raf oncogene homolog B1 antibody NS7 antibody Oncogen BRAF antibody p94 antibody Proto-oncogene B-Raf antibody Proto-oncogene c-Rmil antibody RAFB1 antibody RMIL antibody Serine/threonine-protein kinase B-raf antibody v-Raf murine sarcoma viral oncogene homolog B1 antibody |
Protein information supplied by UniProt
Product | Note | Status | Price | |
---|---|---|---|---|
|
BRAF-101AP | |||
|
BRAF-FITC | |||
|
P-BRAF | |||
|
PC-BRAF | |||
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