General Information

Product

GPCR GPR87 Antibody

Description

Affinity Purified G-protein coupled receptor 87 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Panda, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-60 on human GPCR87 protein.

Accession #

NCBI: NP_076404

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9BY21

Overview

Receptor for lysophosphatidic acid.  Necessary for p53/TP53-dependent survival in response to DNA damage.

Molecular Function

G-protein coupled receptor, Receptor, Transducer

Subcellular Location

Cell membrane; Multi-pass membrane protein

Expression

Placenta and prostate. Weaker expression in thymus. Overexpressed in squamous cell carcinoma of the lung.

Structure

Belongs to G-protein coupled receptor 1 family

Alternative Nomenclature

FKSG 78 antibody

G-protein coupled receptor 87 antibody

G-protein coupled receptor 95 antibody

GPR 95 antibody

hGPCR87 antibody

KPG_002 antibody

MGC131898 antibody

Orphan GPCR 87 antibody

Probable G protein coupled receptor 87 antibody

General Information

Product

Helicobacter pylori Antibody

Verified Applications

CM, ELISA, ICC, IF, IP, IHC, WB

Host

Rabbit

Species Cross Reactivity

Helicobacter pylori

Immunogen

Heat killed and denatured Helicobacter pylori extracts

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.80-1.35 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

ELISA

1:5,000

Immunocytochemistry

1:200

Immunofluorescence

1:300

Immunohistochemistry

1:200

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q96CV9

Overview

Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection.

Molecular Function

polyubiquitin binding, Rab GTPase binding, protein C-terminus binding

Subcellular Location

Cytoplasm › perinuclear region. Golgi apparatus. Golgi apparatus › trans-Golgi network. Cytoplasmic vesicle ›autophagosome. Cytoplasmic vesicle. Recycling endosome

Expression

Present in aqueous humor of the eye (at protein level). Highly expressed in trabecular meshwork. Expressed nonpigmented ciliary epithelium, retina, brain, adrenal cortex, fetus, lymphocyte, fibroblast, skeletal muscle, heart, liver, brain and placenta.

Structure

Interacts with HD, Rab8 (RAB8A and/or RAB8B) (active GTP-bound form), GTF3A, TRAF3, TBK1, MYO6 and TFRC. Binds to linear ubiquitin chains. Interacts with LC3 family members MAP1LC3A, MAP1LC3B, GABARAP, GABARAPL1 and GABARAPL2; OPTN phosphorylation increas

Alternative Nomenclature

H. pylori antibody

General Information

Product

Phospho-alpha Actinin 4 (S159) antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic phospho-peptide, corresponding to a region within amino acid regions 140-190 of Human alpha Actinin 4 conjugated to a carrier protein.

Accession #

NCBI:  NP_004915

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500 Predicted molecular weight: 105kDa

Protein

Uniprot #

O43707

Overview

F-actin cross-linking protein that anchors variety of intracellular structures.  Bundling protein involved in vesicular trafficking via its association with the CART complex.

Molecular Function

Protein Transport; Actin-binding

Subcellular Location

Cytoplasmic mRNP granules containing untranslated mRNAs.  Colocalizes with actin stress fibers.

Expression

Induced by pI3 kinase inhibitor wortmannin or by cytochalasin D.  Localized in the nucleus.

Structure

Widely Expressed

Alternative Nomenclature

actinin 4 antibody

Actinin alpha 4 antibody

ACTN 4 antibody

DKFZp686K23158 antibody

F actin cross linking protein antibody

Focal segmental glomerulosclerosis 1 antibody

FSGS 1 antibody

General Information

Product

SOCS3 Antibody

Description

Affinity Purified Suppresor of Cytokine Signaling 3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 175-225 on human SOCS3 protein.

Accession #

GenBank:  CAG46495

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q6FI39

Molecular Function

Protein modification; protein ubiquitination.

Subcellular Location

Cytoplasm

Expression

Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.

Alternative Nomenclature

ATOD4 antibody

CIS 3 antibody

Cish3 antibody

Cytokine induced SH2 protein 3 antibody

E2a Pbx1 target gene in fibroblasts 10 antibody

EF 10 antibody

SOCS 3 antibody

SSI3 antibody

STAT induced STAT inhibitor 3 antibody

Suppressor of cytokine signaling 3 antibody

General Information

Product

STIM2 Antibody Affinity Purified

Description

Affinity Purified STIM2 protein Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 600-650 on human STIM2 protein.

Accession #

Gen Bank: AAI36450.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.51-0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9P246

Overview

Plays a role in mediating store-operated Ca2+ entry, a Ca2+ influx following depletion of intracellular Ca2+ stores. Regulates basal cytosolic and endoplasmic reticulum Ca2+ concentrations.

Molecular Function

Calcium channel regulator activity, calcium ion binding, store-operated calcium channel activity

Subcellular Location

Endoplasmic reticulum membrane, single-pass type I membrane protein. (Note: Dynamically translocates from a uniform endoplasmic reticulum distribution to punctual endoplasmic reticulum-plasma membrane junctions in response to decrease in endoplasmic retic

Expression

Expressed in all tissues and tumor cell lines examined.

Alternative Nomenclature

FLJ39527 antibody

KIAA1482 antibody

stromal interaction molecule 2 antibody

General Information

Product

hopQ Antibody

Description

Affinity Purified hopQ H. Pylori Antibody

Verified Applications

CM, ELISA, ICC, IF, IP, IHC, WB

Host

Rabbit

Species Cross Reactivity

Heliobacter pylori

Immunogen

Heat killed and denatured Heliocobacter pylori extracts

Accession #

Accession: WP_202143412

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.80-1.35 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

ELISA

1:5,000

Immunocytochemistry

1:200

Immunofluorescence

1:300

Immunohistochemistry

1:200

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

J0TJV0

Overview

Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection.

Molecular Function

polyubiquitin binding, Rab GTPase binding, protein C-terminus binding

Subcellular Location

Cytoplasm › perinuclear region. Golgi apparatus. Golgi apparatus › trans-Golgi network. Cytoplasmic vesicle ›autophagosome. Cytoplasmic vesicle. Recycling endosome

Expression

Present in aqueous humor of the eye (at protein level). Highly expressed in trabecular meshwork. Expressed nonpigmented ciliary epithelium, retina, brain, adrenal cortex, fetus, lymphocyte, fibroblast, skeletal muscle, heart, liver, brain and placenta.

Structure

Interacts with HD, Rab8 (RAB8A and/or RAB8B) (active GTP-bound form), GTF3A, TRAF3, TBK1, MYO6 and TFRC. Binds to linear ubiquitin chains. Interacts with LC3 family members MAP1LC3A, MAP1LC3B, GABARAP, GABARAPL1 and GABARAPL2; OPTN phosphorylation increas

Alternative Nomenclature

H. pylori antibody

General Information

Product

Outer membrane protein 26 Antibody

Description

Affinity Purified Outer membrane protein 26 H. Pylori Antibody

Verified Applications

CM, ELISA, ICC, IF, IP, IHC, WB

Host

Rabbit

Species Cross Reactivity

Heliobacter pylori

Immunogen

Heat killed and denatured Heliocobacter pylori extracts.

Accession #

Accession: WP_097717672

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.80-1.35 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

ELISA

1:5,000

Immunocytochemistry

1:200

Immunofluorescence

1:300

Immunohistochemistry

1:200

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

J0NP37

Overview

Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection.

Molecular Function

polyubiquitin binding, Rab GTPase binding, protein C-terminus binding

Subcellular Location

Cytoplasm › perinuclear region. Golgi apparatus. Golgi apparatus › trans-Golgi network. Cytoplasmic vesicle ›autophagosome. Cytoplasmic vesicle. Recycling endosome

Expression

Present in aqueous humor of the eye (at protein level). Highly expressed in trabecular meshwork. Expressed nonpigmented ciliary epithelium, retina, brain, adrenal cortex, fetus, lymphocyte, fibroblast, skeletal muscle, heart, liver, brain and placenta.

Structure

Interacts with HD, Rab8 (RAB8A and/or RAB8B) (active GTP-bound form), GTF3A, TRAF3, TBK1, MYO6 and TFRC. Binds to linear ubiquitin chains. Interacts with LC3 family members MAP1LC3A, MAP1LC3B, GABARAP, GABARAPL1 and GABARAPL2; OPTN phosphorylation increas

Alternative Nomenclature

H. pylori antibody

General Information

Product

MAPK14 Antibody

Description

Affinity Purified p38 Mitogen Activated Protein Kinase 14 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-200 on human Mitogen Activated Protein Kinase 14 protein.

Accession #

GenBank: ACI00233.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.69 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q16539

Overview

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation.

Molecular Function

ATP binding; MAP kinase activity; MAP kinase kinase activity; NFAT protein binding; Protein serine/threonine kinase activity

Subcellular Location

Cytoplasm; Nucleus

Structure

Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR (By similarity). This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation (By similarity). Interacts with SPAG9 and GADD45A (By similarity). Interacts with CDC25B, CDC25C, DUSP1, DUSP10, DUSP16, NP60, SUPT20H and TAB1. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B.

Alternative Nomenclature

CSAID-binding protein antibody

CSBP antibody

Cytokine suppressive anti-inflammatory drug-binding protein antibody

EXIP antibody

MAP kinase 14 antibody

MAP kinase p38 alpha antibody

MAPK14 antibody

MAX-interacting protein 2 antibody

Mitogen-activated protein kinase 14 antibody

p38 antibody

p38 MAP kinase antibody

SAPK2A antibody

Stress-activated protein kinase 2a antibody

General Information

Product

ENPP2 Antibody

Description

Affinity Purified ectonucleotide pyrophosphatase/phosphodiesterase 2 (Autotaxin) Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 778-818 on human Ectonucleotide pyrophosphatase/phosphodiesterase 2 (autotaxin) protein.

Accession #

GenBank: EAW91988.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-0.95 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:250

Protein

Uniprot #

UPI0000EE3297

Overview

Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor.

Molecular Function

alkylglycerophosphoethanolamine phosphodiesterase activity; calcium ion binding; hydrolase activity; lysophospholipase activity; nucleic acid binding; nucleotide diphosphatase activity; phosphodiesterase I activity; polysaccharide binding; scavenger receptor activity; transcription factor binding; zinc ion binding

Subcellular Location

Secreted

Expression

Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues.

Structure

Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.

Contains 2 SMB (somatomedin-B) domains.

Alternative Nomenclature

ATX antibody

ATX X antibody

Autotaxin antibody

Autotaxin t antibody

Ectonucleotide pyrophosphatase/phosphodiesterase 2 antibody

Enpp2 antibody

Extracellular lysophospholipase D antibody

FLJ26803 antibody

LysoPLD antibody

NPP2 antibody

PD IALPHA antibody

PDNP2 antibody

Phosphodiesterase I alpha antibody

Plasma lysophospholipase D antibody

General Information

Product

Phospho PDE3B Antibody

Description

Affinity Purified Phosphorylated Phosphodiesterase 3B Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 260-310 on rat PDE3B protein. Selective for only PDE3B variant

Accession #

NCBI:  EDM17772.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.0-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

UPI000154F033

Overview

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3.

Molecular Function

Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Subcellular Location

Membrane; Multipass membrane protein

Expression

Abundant in adipose tissues.

Structure

Interacts with PIK3CG. Interacts with RAPGEF3 and PIK3R6 (By similarity).

Alternative Nomenclature

5''-cyclic phosphodiesterase B antibody

Adipocyte cAMP phosphodiesterase cGMP inhibited antibody

AI847709 antibody

cAMP Phosphodiesterase, adipocyte, cGMP-inhibited antibody

CGI-PDE B antibody

CGIP1 antibody

CGIPDE1 antibody

cGMP inhibited 3 5 cyclic phosphodiesterase B antibody

HcGIP1 antibody

Pde3b antibody

Phosphodiesterase 3B cGMP inhibited antibody