General Information

Product

GADD34 Antibody

Description

Affinity Purified Growth arrest and DNA damage-inducible protein GADD34 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Predicted: Bovine, Monkey

Immunogen

Synthetic peptide corresponding to internal sequence of amino acids 380-400 of Human GADD34, covalently modified and coupled to a carrier protein.

Sequence:

PATGVFLKSWVYQPGEDTEEE

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:800-1:1,000

ELISA

1:800-1:1,000

Western Blot

1:500

Protein

Uniprot #

O75807

Overview

Recruits serine/threonine-protein phosphatase PP1 to dephosphorylate translation initation factor eIF2A/EIF2S1.  This reverses shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress.

Molecular Function

Apoptosis; Stress Response; Translation regulation

Subcellular Location

Endoplasmic Reticulum membrane; Peripheral membrane protein; Cytoplasmic side; Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic Side

Expression

induced by methyl methanesulfonate and ionizing irradiation.  Apoptosis is induced by interluekin 24 in melanoma cells

Structure

belongs to PPP1R15 family.

Alternative Nomenclature

Apoptosis associated protein antibody

GADD 34 antibody

Growth arrest and DNA-damage-inducible 34 antibody

Myeloid differentiation primary response protein MyD116 homolog antibody

PPP1R15A antibody

Protein phosphatase 1 regulatory (inhibitor) subunit 15A antibody

General Information

Product

Phospho-ACK1 Antibody Antibodyd

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-300 on ACK1 protein. 

Accession #

GenBank: AAL55732.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

Dot Blot

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P13749

Overview

Plays a role in presenting foreign antigens to the immune system.

Molecular Function

peptide antigen bonding

Subcellular Location

Membrane; Single-pass type I membrane protein

Structure

Heterodimer of an alpha chain and a beta chain

Alternative Nomenclature

Acetate kinase 1 antibody

ACK 1 antibody

ACK antibody

Activated Cdc42 associated kinase 1 antibody

Activated p21cdc42Hs kinase antibody

FLJ44758 antibody

FLJ45547 antibody

p21cdc42Hs antibody

TNK 2 antibody

Tyrosine kinase non receptor 2 antibody

General Information

Product

PTPD1 Antibody 

Description

Affinity Purified PTPN21 protein Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-500 on mouse PTPND1 protein.

Accession #

GenBank: AAH98500.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.87 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:250

Protein

Uniprot #

Q4KML9

Overview

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Molecular Function

Hydrolase, Protein Phosphatase, Protein tyrosine Phosphatase activity

Subcellular Location

Cytoskeleton

Alternative Nomenclature

protein tyrosine phosphatase D1 antibody

Protein tyrosine phosphatase non receptor type 21 antibody

PTPNL antibody

PTPRL10 antibody

General Information

Product

Slingshot homolog 1 Antibody Affinity Purified

Description

Affinity Purified Protein phosphatase Slingshot homolog 1 isoform 1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on Slingshot homolog 1 protein.

Accession #

NCBI:  NP_061857.3

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8WYL5

Overview

Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.

Molecular Function

Hydrolase, protein phosphatase

Subcellular Location

Cytoplasm, cytoskeleton, cell projection, lamellipodium, cleavage furrow, midbody. (Note: Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at site of cell locomotion. Also localized to

Alternative Nomenclature

AW551225 antibody

Coro1c antibody

coronin, actin binding protein 1C antibody

Gm1394 antibody

Gm1395 antibody

Protein phosphatase Slingshot homolog 1 antibody

slingshot like 1 antibody

SSH1 antibody

General Information

Product

MINPP1 Antibody

Description

Affinity Purified Multiple inositol polyphosphate phosphatase-like protein Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-100 on rat MINPP1 protein.

Accession #

GenBank: BAD93056.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.51-0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9UNW1

Subcellular Location

Endoplasmic reticulum lumen

Expression

Detected widely, with a high level of expression in kidney, liver and placenta.

Alternative Nomenclature

3 antibody

4 antibody

5)-tetrakisphosphate 3-phosphatase antibody

DKFZp564L2016 antibody

HIPER1 antibody

Inositol (1 antibody

inositol (1,3,4,5)-tetrakisphosphate 3-phosphatase antibody

Minpp1 antibody

MINPP2 antibody

MIPP antibody

Multiple inositol polyphosphate phosphatase 1 antibody

General Information

Product

VPS29 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 133-183 on rat Vacuolar Protein Sorting-Associated Protein 29.

Accession #

NCBI:  NP_001099402.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-1.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9UBQ0

Overview

Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3.

Molecular Function

Metal ion binding, Phosphoserine phosphatase activity

Subcellular Location

Cytoplasm. Membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Early endosome Curated. Late endosome.

Expression

Ubiquitous. Highly expressed in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon skeletal muscle, kidney and brain.

Structure

Component of the heterotrimeric retromer cargo-selective complex (CSC), also described as vacuolar protein sorting subcomplex (VPS), formed by VPS26 (VPS26A or VPS26B), VPS29 and VPS35 (PubMed:11102511, PubMed:16737443, PubMed:17891154). The CSC has a highly elongated structure with VPS26 and VPS29 binding independently at opposite distal ends of VPS35 as central platform. The CSC is believed to associate with variable sorting nexins to form functionally distinct retromer complex variants.

Alternative Nomenclature

DC15 antibody

DC7 antibody

DKFZp564F0223 antibody

FLJ20492 antibody

hVPS29 antibody

PEP11 antibody

Retromer protein antibody

Vacuolar protein sorting 29 antibody

Vacuolar sorting protein VPS29/PEP11 antibody

Vesicle protein sorting 29 antibody

VPS 29 antibody

x 007 protein antibody

General Information

Product

Phospho Slingshot homolog 1 Antibody

Description

Affinity Purified Phosphorylated Protein phosphatase Slingshot homolog 1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Phosphorylated synthetic peptide corresponding to unique amino acid sequence on Slingshot homolog 1 protein.

Accession #

NCBI:  NP_061857.3

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8WYL5

Overview

Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.

Molecular Function

Hydrolase, protein phosphatase

Subcellular Location

Cytoplasm, cytoskeleton, cell projection, lamellipodium, cleavage furrow, midbody. (Note: Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at site of cell locomotion.

Alternative Nomenclature

AW551225 antibody

Coro1c antibody

coronin, actin binding protein 1C antibody

Gm1394 antibody

Gm1395 antibody

Protein phosphatase Slingshot homolog 1 antibody

slingshot like 1 antibody

SSH1 antibody

General Information

Product

PTP alpha Antibody

Description

Affinity Purified Receptor-type tyrosine-protein phosphatase Alpha Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 650-700 on human Receptor-type tyrosine-protein phosphatase Alpha protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P18433

Overview

H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphate

Molecular Function

protein tyrosine phosphatase activity

Ras guanyl-nucleotide exchange factor activity

transmembrane receptor protein tyrosine phosphatase activity

Subcellular Location

Membrane

Structure

Belongs to the protein-tyrosine phosphatase family. Receptor class 4 subfamily.

Alternative Nomenclature

HEPTP antibody

HLPR antibody

HPTP alpha antibody

HPTPA antibody

HPTPalpha antibody

Leukocyte common antigen related peptide antibody

LRP antibody

Protein tyrosine phosphatase alpha antibody

Protein tyrosine phosphatase receptor type A antibody

Protein tyrosine phosphatase receptor type alpha polypeptide antibody

Protein tyrosine phosphate antibody

Protein-tyrosine phosphatase alpha antibody

PTPA antibody

PTPalpha antibody

PTPase alpha antibody

PTPLCA related phosphatase antibody

Ptpra antibody

PTPRL 2 antibody

PTRA antibody

R-PTP-alpha antibody

Receptor-type tyrosine-protein phosphatase alpha antibody

RPTPA antibody

Tyrosine phosphatase alpha antibody

General Information

Product

CD45 Antibody

Description

Affinity Purified Receptor-type tyrosine-protein phosphatase C Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 1100-1150 on human Receptor-type tyrosine-protein phosphatase C.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P08575

Subcellular Location

Cell membrane, Membrane

Structure

Repeat, Signal, Transmembrane, Transmembrane helix

Alternative Nomenclature

B220 antibody
CD45 antigen antibody
CD45R antibody
GP180 antibody
LCA antibody
Leukocyte common antigen antibody
loc antibody
Ly-5 antibody
LY5 antibody
Lyt-4 antibody
Protein tyrosine phosphatase receptor type c polypeptide antibody
Ptprc antibody
Receptor-type tyrosine-protein phosphatase C antibody
T200 antibody
T200 glycoprotein antibody
T200 leukocyte common antigen antibody

General Information

Product

PP2A-alpha Antibody

Description

Affinity Purified Serine / Threonine-Protein Phosphatase 2A , B56- Alpha subunit Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide taken within amino acid region 410-486 on human Serine / Threonine-Protein Phosphatase 2A , B56- Alpha subunit.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q15172

Overview

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

Molecular Function

kinase binding

phosphoprotein phosphatase activity

protein phosphatase activator activity

protein phosphatase regulator activity

Subcellular Location

Centromere, Chromosome, Cytoplasm, Nucleus

Expression

Widely expressed with the highest expression in heart and skeletal muscle.

Structure

Belongs to the phosphatase 2A regulatory subunit B56 family.

Alternative Nomenclature

PP2A alpha antibody
PP2AA_HUMAN antibody
PP2Aalpha antibody
PP2Ac antibody
PP2CA antibody
PP2Calpha antibody
PPP2CA antibody
Protein phosphatase 2 (formerly 2A) catalytic subunit alpha isoform antibody
Protein phosphatase 2 catalytic subunit alpha isoform antibody
Protein phosphatase 2, catalytic subunit, alpha isozyme antibody
Protein phosphatase 2A catalytic subunit, alpha isoform antibody
Replication protein C antibody
RP C antibody
RP-C antibody
RPC antibody
Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody