EPAC1 Antibody

Product no.: EPAC-112AP

General Information
Product	Epac1 Antibody
Description	Affinity Purified Rap Guanine Nucleotide Exchange Factor 3 Antibody Mid-Region
Verified Applications	CM, ELISA, ICC, IF, IHC, WB
Host	Rabbit
Species Cross Reactivity	Horse, Human, Mouse, Rat
Immunogen	Synthetic peptide corresponding to amino acids 526-541 of Epac1 protein, peptide was covalently modified. Sequence DHSVLTLQLQLPVTASVR
Specificity	Does not cross react with Epac2.
Accession #	GenBank: AAD12740.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.59 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Determinant	Mid-Region
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:200
DB	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:200
Immunofluorescence	1:200
Immunohistochemistry	1:200
Western Blot	1:500. Predicted molecular weight: 99 kDa
Protein
Uniprot #	O95398
Overview	Guanine nucleotide exchange factor for RAP1A and RAP2A small GTPases that are activated by binding cAMP
Molecular Function	cAMP-binding; Nucleotide-binding
Subcellular Location	Endomembrane system
Expression	Widely expressed with highest levels in kidney, heart, thyroid and brain
Structure	Interacts with PDE3B
Alternative Nomenclature	bcm910 antibody CGEF 1 antibody Epac 1 antibody EPAC antibody Exchange protein directly activated by cAMP 1 antibody MGC21410 antibody RAP guanine nucleotide exchange factor antibody RAPGEF3 antibody

Protein information supplied by UniProt

Fabgennix Epac1 Antibody has been used in 1 Publication

McPartlin, L.A., P.E. Visconti, and S.J. Bedford-Guaus. “Guanine-Nucleotide Exchange Factors (RAPGEF3/RAPGEF4) Induce Sperm Membrane Depolarization and Acrosomal Exocytosis in Capacitated Stallion Sperm.”Biology of Reproduction 85.1 (2011): 179–188. PMC3123385

Confocal Microscopy, Immunofluorescence, Western Blot; Horse, Mouse

Publishing research using EPAC-101AP? Please let us know, we can cite the reference in this datasheet.

Customer ratings for EPAC1 Antibody

Number of ratings: 1
Average rating: 5

Excellent
from Verified Customer on 01/12/2021

Western blot using rabbit cell lysate whole cells (pancreatic acini). 20 µg rabbit pancreatic acini loaded on 7.5% reduced denaturing gel. 5% milk was used as blocking agent for 1 hour at 25°C. 1/1000 primary antibody dilution for 16 hours in 5% BSA at 4°C. Specific 99 kDa band was found.

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