General Information

Product

AMPK alpha 2 Antibody

Description

Affinity Purified 5'-AMP-activated protein kinase catalytic subunit alpha-2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 480-530 on rat 5'-AMP-activated protein kinase catalytic subunit alpha-2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q09137

Overview

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators.

Molecular Function

[acetyl-CoA carboxylase] kinase activity

[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity

AMP-activated protein kinase activity

ATP binding Source: RGD

chromatin binding

histone serine kinase activity

metal ion binding -KW

protein binding

Subcellular Location

Nucleus, Cytoplasm

Expression

Skeletal muscle, lower levels in liver, heart and kidney.

Structure

The AIS (autoinhibitory sequence) region shows some sequence similarity with the ubiquitin-associated domains and represses kinase activity.

Alternative Nomenclature

5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody

ACACA kinase antibody

Acetyl-CoA carboxylase kinase antibody

AMPK alpha 2 chain antibody

AMPK subunit alpha-2 antibody

AMPK2 antibody

AMPKa2 antibody

HMGCR kinase antibody

Hydroxymethylglutaryl-CoA reductase kinase antibody

PRKAA antibody

PRKAA2 antibody

Protein kinase AMP activated alpha 2 catalytic subunit antibody

General Information

Product

DWORF Antibody

Description

Affinity Purified Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator DWORF Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synththetic peptide corresponding to full length Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator DWORF protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P0DN83

Overview

Enhances the activity of ATP2A1/SERCA1 ATPase in sarcoplasmic reticulum by displacing ATP2A1/SERCA1 inhibitors, thereby acting as a key regulator of skeletal muscle activity. Does not directly stimulates SERCA pump activity. Enhances sarcoplasmic reticulum Ca2+ uptake and myocyte contractility by displacing the SERCA inhibitory peptides sarcolipin (SLN), phospholamban (PLN) and myoregulin (MRLN).

Molecular Function

Sarcoplasmic reticulum membrane; Single-pass membrane protein

Alternative Nomenclature

Dwarf open reading frame antibody

DWORF antibody

Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator

DWORFSERCA regulator DWORF antibody

General Information

Product

Angiopoietin-like 4 Antibody

Description

Affinity Purified Angiopoietin-related protein 4 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse

Immunogen

Synthetic peptide taken within amino acid region 50-100 on mouse Angiopoietin-related protein 4.

Accession #

NCBI:  NP_065606.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q9Z1P8

Overview

Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity (By similarity). In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.

Molecular Function

enzyme inhibitor activity

identical protein binding

Subcellular Location

Secreted

Expression

Predominantly expressed in adipose tissue and is strongly up-regulated by fasting in white adipose tissue and liver.

Structure

Coiled coil, Signal

Alternative Nomenclature

Angiopoietin like 4 antibody

Angiopoietin-like protein 4 antibody

ANGPT L2 antibody

ANGPT L4 antibody

Angptl4 antibody

ARP4 antibody

Fasting induced adipose factor antibody

FIAF antibody

HARP antibody

Hepatic angiopoietin related protein antibody

Hepatic fibrinogen/angiopoietin-related protein antibody

HFARP antibody

NL2 antibody

Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein antibody

PGAR antibody

pp1158 antibody

PPARG angiopoietin related protein antibody

PSEC0166 antibody

TGQTL antibody

UNQ171 antibody

Weakly similar to angiopoietin 1 [H.sapiens] antibody

General Information

Product

GBA Antibody

Description

Affinity Purified Glucosylceramidase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Recombinant Glucosylceramidase protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P04062

Overview

Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose (PubMed:9201993, PubMed:24211208). Thereby, plays a central role in the degradation of complex lipids and the turnover of cellular membranes (PubMed:27378698). Through the production of ceramides, participates to the PKC-activated salvage pathway of ceramide formation (PubMed:19279011). Also plays a role in cholesterol metabolism (PubMed:24211208, PubMed:26724485). May either catalyze the glucosylation of cholesterol, through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol (PubMed:24211208, PubMed:26724485). The short chain saturated C8:0-GlcCer and the mono-unsaturated C18:0-GlcCer being the most effective glucose donors for that transglucosylation reaction (PubMed:24211208). Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide (PubMed:26724485). Finally, may also hydrolyze cholesteryl-beta-D-glucoside to produce D-glucose and cholesterol (PubMed:24211208, PubMed:26724485).

Molecular Function

glucosylceramidase activity

glucosyltransferase activity

hydrolase activity

scavenger receptor binding

signaling receptor binding

steryl-beta-glucosidase activity

Subcellular Location

Lysosome

Expression

Interacts with saposin-C (PubMed:10781797). Interacts with SCARB2 (PubMed:18022370). Interacts with TCP1 (PubMed:21098288). May interact with SNCA; may inhibit the glucosylceramidase activity (PubMed:23266198). Interacts with GRN; this interaction prevents aggregation of GBA-SCARB2 complex via interaction with HSPA1A upon stress (PubMed:27789271).

Structure

Belongs to the glycosyl hydrolase 30 family.

Alternative Nomenclature

Lysosomal acid glucosylceramidase

General Information

Product

GAPDH Antibody

Description

Affinity Purified Glyceraldehyde-3-Phosphate dehydrogenase isoform 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-200 on mouse Glyceraldehyde-3-phosphate dehydrogenase protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P16858

Overview

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively

Molecular Function

Oxidoreductase, Transferase

Subcellular Location

Cytoplasm, Cytoskeleton, Nucleus

Expression

brain;cerebullum;heart;kidney;liver;skeletal muscle

Structure

Homotetramer

Alternative Nomenclature

AI747587 antibody

cytoplasmic antibody

EC 1.1.1.8 antibody

FLJ26652 antibody

G3PD antibody

Gdc-1 antibody

Gdp1 antibody

Glycerol 3 phosphate dehydrogenase 1 antibody

Glycerol 3 phosphate dehydrogenase cytosolic antibody

Glycerol 3 phosphate dehydrogenase soluble antibody

Glycerol-3-phosphate dehydrogenase [NAD+] antibody

Glycerphosphate dehydrogenase antibody

GPD-C antibody

gpd1 antibody

Gpd3 antibody

GPDA_HUMAN antibody

Gpdc antibody

GPDH antibody

GPDH-C antibody

HTGTI antibody

KIAA4010 antibody

MGC93453 antibody

MGPD antibody

mKIAA4010 antibody

General Information

Product

GAPDH Antibody

Description

Affinity Purified Glyceraldehyde-3-Phosphate dehydrogenase isoform 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 250-300 on human Glyceraldehyde-3-phosphate dehydrogenase protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P04406

Overview

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively

Molecular Function

Oxidoreductase, Transferase

Subcellular Location

Cytoplasm, Cytoskeleton, Nucleus

Expression

brain;cerebullum;heart;kidney;liver;skeletal muscle

Structure

Homotetramer

Alternative Nomenclature

AI747587 antibody

cytoplasmic antibody

EC 1.1.1.8 antibody

FLJ26652 antibody

G3PD antibody

Gdc-1 antibody

Gdp1 antibody

Glycerol 3 phosphate dehydrogenase 1 antibody

Glycerol 3 phosphate dehydrogenase cytosolic antibody

Glycerol 3 phosphate dehydrogenase soluble antibody

Glycerol-3-phosphate dehydrogenase [NAD+] antibody

Glycerphosphate dehydrogenase antibody

GPD-C antibody

gpd1 antibody

Gpd3 antibody

GPDA_HUMAN antibody

Gpdc antibody

GPDH antibody

GPDH-C antibody

HTGTI antibody

KIAA4010 antibody

MGC93453 antibody

MGPD antibody

mKIAA4010 antibody