General Information
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Product
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PDE4D3 Antibody
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Description
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Affinity Purified cAMP-specific phoshodiesterase HPDE4D3 variant Antibody
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Verified Applications
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CM, ELISA, ICC/IF, IHC, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Chicken, Mouse, Rat
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Immunogen
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Synthetic peptide corresponding to unique amino acid sequence on PDE4D3 variant.
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Specificity |
This antibody does not cross react with PDE4D1, PDE4D2, PDE4D4, PDE4D5. Does not cross react with other PDE family members such as PDE4A, PDE4B or PDE4C. |
Accession #
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GenBank: EAW74861.1
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.68 µg/µl in antibody stabilization buffer
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Form
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Affinity Purified
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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Confocal Microscopy
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1:200
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DB
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1:10,000
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ELISA
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1:10,000
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Immunocytochemistry
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1:200
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Immunofluorescence
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1:200
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Immunohistochemistry
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1:200
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Immunoprecipitation
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1:150
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Western Blot
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1:500
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Protein
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Uniprot #
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Q08499
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Overview
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Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
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Molecular Function
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Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding
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Subcellular Location
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Apical cell membrane, Cytoplasm, Membrane
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Expression
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Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
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Structure
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Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1).
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Alternative Nomenclature
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DPDE3 antibody
hPDE4D3 antibody
HSPDE4D antibody
PDE43 antibody
PDE4D antibody
PDE4DN2 antibody
Phosphodiesterase 4D antibody
Phosphodiesterase 4D cAMP-specific antibody
Phosphodiesterase Type 4D3 antibody
STRK1 antibody
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