General Information

Product

Brk Antibody

Description

Affinity Purified Protein-Tyrosine Kinase 6 isoform Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-450 on mouse Protein-Tyrosine Kinase 6 isoform.

Accession #

NCBI:  NP_033210

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polylclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q64434

Molecular Function

ATP binding

identical protein binding

non-membrane spanning protein tyrosine kinase activity

protein tyrosine kinase activity

signaling receptor binding

Subcellular Location

Nucleus, Cytoplasm, Membrane, Ruffle

Expression

Expressed only in epithelial tissues, including the skin and lining of the alimentary canal. Restricted to the cell layers immediately above the proliferative cell zone in these epithelia.

Structure

The SH3 domain plays a major role in substrate interactions. The SH2 domain of PTK6 plays a role in protein-protein interactions, but is likely more important for the regulation of catalytic activity (By similarity).

Alternative Nomenclature

Breast tumor kinase antibody

Protein tyrosine kinase 6 antibody

Ptk6 antibody

Tyrosine-protein kinase BRK antibody

General Information

Product

ABL2 Antibody

Description

Affinity Purified Tyrosine-protein kinase ABL2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 1100-1150 on human Tyrosine-protein kinase ABL2 / ARG.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P42684

Overview

Non-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35.

Molecular Function

actin filament binding

actin monomer binding

ATP binding

magnesium ion binding

manganese ion binding

non-membrane spanning protein tyrosine kinase activity

phosphotyrosine residue binding

protein kinase activity

protein tyrosine kinase activity

Subcellular Location

Cytoskeleton

Expression

Widely expressed.

Alternative Nomenclature

Abelson murine leukemia viral oncogene homolog 2 antibody

Abelson related gene protein antibody

Abelson tyrosine-protein kinase 2 antibody

Abelson-related gene protein antibody

ABL2 antibody

ABL2_HUMAN antibody

ABLL antibody

ARG antibody

Tyrosine kinase ARG antibody

Tyrosine protein kinase ABL2 antibody

Tyrosine-protein kinase ARG antibody

v abl Abelson murine leukemia viral oncogene homolog 2 antibody