General Information

Product

PDE4D1 Antibody

Description

Affinity Purified Phosphodiesterase 4D1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Mouse, Rat

Immunogen

Synthetic peptide from unique region near N-terminus of PDE4D1 variant.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.73 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Function

Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Subcellular Location

Apical cell membrane, Cytoplasm, Membrane

Expression

Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.

Structure

Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protei

Alternative Nomenclature

cAMP specific 3',5' cyclic phosphodiesterase 4D antibody

DKFZp686M11213 antibody

5''-cyclic phosphodiesterase 4D antibody

cAMP specific phosphodiesterase 4D antibody

cAMP specific phosphodiesterase PDE4D antibody

DPDE 3 antibody

Dunce like phosphodiesterase E3 antibody

FLJ97311 antibody

HSPDE4D antibody

PDE 43 antibody

PDE4DN2 antibody

Phosphodiesterase E3 dunce homolog Drosophila antibody

STRK1 antibody

General Information

Product

PDE4 Antibody

Description

Phosphodiesterase 4 Antibody

Verified Applications

ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide common to most PDE4 subtypes: PDE4A, PDE4D, PDE4B

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

Dot Blot

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500-1:1,000

Protein

Overview

Phosphodiesterase specific for extracellular cAMP. Involved in the degradation of extracellular cAMP specifically during multicellular development.

Molecular Function

3',5'-cyclic-nucleotide phosphodiesterase activity; cAMP catabolic process; signal transduction; Hydrolase

Subcellular Location

Cell membrane; Multipass membrane protein

Expression

Low expression during growth and cell aggregation (up to 6 hours), maximally increases after 9 hours of starvation, during the mound stage. Remains expressed at significant levels during the other stages of multicellular development.

Structure

Belongs to the cyclic nucleotide phosphodiesterase family.

Alternative Nomenclature

PDE4A antibody

PDE4B antibody

PDE4C antibody

PDE4D antibody

Phosphodiesterase 4 antibody

Phosphodiesterase 4A antibody

Phosphodiesterase 4B antibody

Phosphodiesterase 4C antibody

Phosphodiesterase 4D antibody

General Information

Product

PDE6 delta / PDE6C Cone Specific Antibody

Description

Affinity Purified cone cGMP phosphodiesterase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-75 on cone-specific PDE6C protein.

Accession #

GenBank: CAA64079.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P51160

Overview

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Molecular Function

Hydrolase, 3',5'-cyclic-GMP phosphodiesterase activity, cGMP binding

Subcellular Location

Cell membrane; Lipid-anchor; Cytoplasmic side

Structure

Composed of two alpha' subunits that are associated with 3 smaller proteins of 11, 13, and 15 kDa.

Alternative Nomenclature

cGMP phosphodiesterase 6C antibody

COD4 antibody

PDE 6C antibody

PDE6 alpha prime antibody

PDEA2 antibody

Phosphodiesterase 6C cGMP specific cone alpha prime antibody

General Information

Product

PDE10A Antibody

Description

Affinity Purified Phosphodiesterase 10A

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide (Human) taken against a common sequence near the 250-325 amino acids downstream of N-terminal region of PDE10A. Synthetic MAP.

Accession #

GenBank: AAD32596.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.7 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100-1:150

Dot Blot

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100-1:150

Immunofluorescence

1:100-1:150

Immunohistochemistry

1:100-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9Y233

Overview

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate.

Molecular Function

Hydrolase, metal-ion binding, cGMP binding

Subcellular Location

Cytoplasm

Expression

Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum.

Structure

Homodimer

Alternative Nomenclature

5''-cyclic phosphodiesterase 10A antibody

cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A antibody

FLJ11894 antibody

HSPDE10A antibody

Phosphodiesterase 10A antibody

General Information

Product

PDE10A2 Antibody

Description

Affinity Purified Phosphodiesterase 10A2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human PDE10A2 protein.

Accession #

GenBank: AAD32596.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9Y233

Overview

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate.

Molecular Function

Hydrolase, metal-ion binding, cGMP binding

Subcellular Location

Cytoplasm

Expression

Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum.

Structure

Homodimer

Alternative Nomenclature

5''-cyclic phosphodiesterase 10A antibody

cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A antibody

cAMP and cAMP-inhibited cGMP 3'' antibody

FLJ11894 antibody

HSPDE10A antibody

Phosphodiesterase 10A antibody

General Information

Product

PDE10A12 Antibody Affinity Purified

Description

Affinity Purified Phosphodiesterase 10A12 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Rat

Immunogen

Synthetic peptide taken within amino acid region 50-120 on PDE10A12 protein.

Accession #

GenBank: BAB16383

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.67-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9Y233

Molecular Function

Hydrolase, metal-ion binding, cGMP binding

Subcellular Location

Cytoplasm

Expression

Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum.

Structure

Homodimer

Alternative Nomenclature

5''-cyclic phosphodiesterase 10A antibody

cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A antibody

cAMP and cAMP-inhibited cGMP 3'' antibody

FLJ11894 antibody

HSPDE 10A antibody

PDE 10A antibody

Phosphodiesterase 10A antibody

Phosphodiesterase 10A12 antibody

General Information

Product

PDE11A3 Antibody Affinity Purified

Description

Affinity Purified Phosphodiesterase 11A3 Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide unique to PDE11A3 variant

Accession #

GenBank: AB038041.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

Q9HCR9

Overview

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP. Catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'-GMP, respectively.

Molecular Function

Hydrolase, metal-ion binding, cGMP binding

Subcellular Location

Cytoplasm

Expression

Isoform 1 is present in prostate, pituitary, heart and liver. It is however not present in testis nor in penis, suggesting that weak inhibition by Tadalafil  is not relevant (at protein level). Isoform 2 may be expressed in testis. Isoform 4 is expressed

Alternative Nomenclature

PDE11A antibody

PDE11A3 antibody

PPNAD2 antibody

General Information

Product

PDE11A4 Antibody Affinity Purified

Description

Affinity Purified Phosphodiesterase 11A4 Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide unique to PDE11A4 variant

Accession #

GenBank: BAB62712.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.70-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

Q9HCR9

Overview

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP. Catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'-GMP, respectively.

Molecular Function

Hydrolase, 3', 5'-cyclic-GMP phosphodiesterase activity, cGMP binding

Subcellular Location

Cytoplasm

Expression

Isoform 1 is present in prostate, pituitary, heart and liver. It is however not present in testis nor in penis, suggesting that weak inhibition by Tadalafil is not relevant (at protein level). Isoform 2 may be expressed in testis. Isoform 4 is expressed i

Alternative Nomenclature

cGMP phosphodiesterase 11A antibody

Pde11a antibody

Pde11A2 antibody

Pde11A3 antibody

PDE11A4 antibody

PPNAD2 antibody

General Information

Product

PDE2A4 Antibody

Description

Affinity Purified cGMP-dependent 3',-5'-cyclic phosphodiesterase isoform PDE2A4 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human PDE2A4 protein.

Accession #

NCBI: NP_001139681.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O00408

Overview

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.  Isoform PDE2A2: Regulates Mitochondrial cAMP Levels and Respiration.

Molecular Function

Hydrolase, calcium channel activity, cAMP binding

Subcellular Location

Isoform PDE2A3: Cell membrane; Lipid-anchor, Isoform PDE2A2: Mitochondrion matrix, Isoform PDE2A1: Cytoplasm, Isoform 5: Mitochondrion

Expression

Expressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas.

Structure

Homodimer

Alternative Nomenclature

5''-cyclic phosphodiesterase antibody

cGMP dependent 3'5' cyclic phosphodiesterase antibody

cGMP stimulated phosphodiesterase 1 antibody

cGMP stimulated phosphodiesterase 4 antibody

cGMP-dependent 3'' antibody

CGS PDE antibody

Cyclic GMP stimulated phosphodiesterase antibody

Human cGMP stimulated 3'5' cyclic nucleotide phosphodiesterase antibody

PDE 2A antibody

PDE 2A4 antibody

Phosphodiesterase 2A cGMP stimulated antibody

General Information

Product

PDE4A4 Antibody

Description

Affinity Purified cAMP specific phosphodiesterase 4A variant pde46 Antibody

Verified Applications

CM, ELISA, ICC/IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rabbit

Immunogen

N-terminus PDE4A4 synthetic cyclic peptide

Accession #

GenBank: EAW74861.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P27815

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Function

Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Subcellular Location

Isoform 1: Cytoplasm › perinuclear region, Isoform 2: Cytoplasm, Cell projection, Isoform 4: Membrane; Peripheral membrane protein Isoform 6 : Cytoplasm › perinuclear region Isoform 7 : Cytoplasm. Membrane

Expression

Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).

Structure

Isoform 1 interacts with LYN. Isoform 2 and isoform 6 interact weakly with LYN. Isoform 1, isoform 2 and isoform 6 interact with ARRB2.

Alternative Nomenclature

DPDE2 antibody

PDE4A antibody

Phosphodiesterase 4 antibody

Phosphodiesterase 4A antibody

Phosphodiesterse Type 4A4 antibody