General Information

Product

PDE9A Antibody

Description

Affinity Purified High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A isoform a Antibody

Verified Applications

CM, ELISA, ICC/IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic cyclic peptide common to all PDE9A variants.

Accession #

NCBI:  NP_002597.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DB

1:100

ELISA

1:100

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

O76083

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction.

Molecular Function

Hydrolase, 3', 5' -cyclic-GMP phosphodiesterase activity

Subcellular Location

Isoform PDE9A1: Cell projection, Cytoplasm, Golgi Apparatus, Endoplasmic reticulum, Isoform PDE9A2: Cell projection, Cytoplasm, Isoform PDE9A3: Cytoplasm, Endoplasmic Reticulum, Isoform PDE9A17: Cytoplasm, Endoplasmic reticulum

Expression

Expressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood. Highest levels in brain, heart, kidney, spleen, prostate and colon. Isoform PDE9A12 is found in prostate.

Alternative Nomenclature

FLJ90181 antibody

HSPDE9A2 antibody

PDE 9A antibody

Pde9a antibody

Phosphodiesterase 9A antibody

phosphodiesterase PDE9A21 antibody

General Information

Product

PDE1A7 Antibody Affinity Purified

Description

Affinity Purified Calcium/Calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A isoform PDE1A7 Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide selective only for PDE1A7 gene.

Accession #

NCBI:  NP_001009978.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500-1:750

Protein

Uniprot #

Q9JLL9

Overview

A divalent metal cation

Molecular Function

Hydrolase, calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity, metal ion binding

Alternative Nomenclature

3' 5' cyclic nucleotide phosphodiesterase antibody

5''-cyclic nucleotide phosphodiesterase 1A antibody

61 kDa Cam PDE antibody

Calcium/calmodulin dependent 3' 5' cyclic nucleotide phosphodiesterase 1A antibody

Cam PDE 1A antibody

HCAM 1 antibody

HSPDE1A antibody

MGC26303 antibody

PDE 1A antibody

Phosphodiesterase 1A antibody

General Information

Product

PDE4A8 Antibody Affinity Purified

Description

Affinity Purified cAMP-specific 3',5'-cyclic phosphodiesterase 4A Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IMM, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide specific only for PDE4A8 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:250

Western Blot

1:1,000

Protein

Uniprot #

P27815

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Function

metal ion binding, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Subcellular Location

Isoform 1: Cytoplasm, perinuclear region; Isoform 2:  Cytoplasm, perinuclear region.  Cell projection, ruffle membrane; Isoform 4:  Peripheral membrane protein; Isoform 6:  Cytoplasm; perinuclear region; Isoform 7:  Cytoplasm, Membrane

Expression

Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Iso

Structure

Isoform 1 interacts with LYN. Isoform 2 and isoform 6 interact weakly with LYN. Isoform 1, isoform 2 and isoform 6 interact with ARRB2.

Alternative Nomenclature

5''-cyclic phosphodiesterase 4A antibody

cAMP specific 3 5 cyclic phosphodiesterase 4A antibody

cAMP-specific 3'' antibody

DPDE2 antibody

dunce antibody

PDE46 antibody

PDE4A antibody

Phosphodiesterase 4 antibody

phosphodiesterase 4A cAMP specific antibody

phosphodiesterase E2 dunce homolog antibody

General Information

Product

Phospho-PDE10A Antibody

Description

Affinity Purified Phosphorylated Phosphodiesterase 10A Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Phosphorylated synthetic MAP common to all PDE10A variants.  Taken within amino acid region 1-50 on human PDE10A protein.

Accession #

GenBank: AAD32596.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.7 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9Y233

Overview

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate.

Molecular Function

Hydrolase, metal-ion binding, cGMP binding

Subcellular Location

Cytoplasm

Expression

Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum.

Structure

Homodimer

Alternative Nomenclature

5''-cyclic phosphodiesterase 10A antibody

cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A antibody

FLJ11894 antibody

HSPDE10A antibody

PDE 10A antibody

Pde10a antibody

Phosphodiesterase 10A antibody

General Information

Product

Phospho-PDE4A Antibody

Description

Affinity Purified Phosphorylated Phosphodiesterase 4A (S140) Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rabbit, Rat

Immunogen

Synthetic peptide corresponding to PKA phosphorylation site on PDE4A long form enzymes. The peptide was synthesized with phosphorylated serine residue. 

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Function

Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Subcellular Location

Apical cell membrane, Cytoplasm, Membrane

Expression

Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.

Structure

Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1).

Alternative Nomenclature

cAMP specific 3',5' cyclic phosphodiesterase 4D antibody

DKFZp686M11213 antibody

5''-cyclic phosphodiesterase 4D antibody

cAMP specific 3' 5' cyclic phosphodiesterase 4D antibody

cAMP specific phosphodiesterase 4D antibody

cAMP specific phosphodiesterase PDE4D6 antibody

cAMP-specific 3'' antibody

cyclic AMP phosphodiesterase antibody

DPDE 3 antibody

FLJ97311 antibody

HSPDE4D antibody

PDE 43 antibody

Pde4d antibody

PDE4DN2 antibody

Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody

Phosphodiesterase 4D cAMP specific

Phosphodiesterase E3 dunce homolog Drosophila antibody

STRK 1 antibody

STRK1 antibody

General Information

Product

Phospho-PDE4D PKA site (S190) Antibody

Description

Affinity Purified Phosphorylated Phosphodiesterase 4D PKA site Antibody

Verified Applications

ELISA, IHC-P, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide selective for PDE4D PKA site taken within amino acid region 50-100 on human PDE4D8 protein. Phosphorylated Serine 190 residue. Derived around the PKA phosphorylation site of serine 190:

R-E-S^P-F-L

Accession #

NCBI:  NP_001184148.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q08499

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Function

Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Subcellular Location

Apical cell membrane, Cytoplasm, Membrane

Expression

Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.

Structure

Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1).

Alternative Nomenclature

cAMP specific 3',5' cyclic phosphodiesterase 4D antibody

DKFZp686M11213 antibody

5''-cyclic phosphodiesterase 4D antibody

cAMP specific 3' 5' cyclic phosphodiesterase 4D antibody

cAMP specific phosphodiesterase 4D antibody

cAMP specific phosphodiesterase PDE4D antibody

cAMP specific phosphodiesterase PDE4D6 antibody

cAMP-specific 3'' antibody

cyclic AMP phosphodiesterase antibody

DPDE3 antibody

Dunce like phosphodiesterase E3 antibody

FLJ97311 antibody

HSPDE4D antibody

PDE43 antibody

PDE4DN2 antibody

Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody

Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila) antibody

Phosphodiesterase 4D cAMP specific antibody

Phosphodiesterase E3 dunce homolog Drosophila antibody

STRK 1 antibody

General Information

Product

Phospho PDE4D ERK site Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Phosphorylated synthetic peptide selective for PDE4D ERK site taken within amino acid ergion 550-600 on human PDE4D8 protein.

Accession #

NCBI:  NP_001184148.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q08499

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Function

Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Subcellular Location

Apical cell membrane, Cytoplasm, Membrane

Expression

Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.

Structure

Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1).

Alternative Nomenclature

cAMP specific 3',5' cyclic phosphodiesterase 4D antibody

DKFZp686M11213 antibody

5''-cyclic phosphodiesterase 4D antibody

cAMP specific 3' 5' cyclic phosphodiesterase 4D antibody

cAMP specific phosphodiesterase 4D antibody

cAMP specific phosphodiesterase PDE4D antibody

cAMP specific phosphodiesterase PDE4D6 antibody

cAMP-specific 3'' antibody

cyclic AMP phosphodiesterase antibody

DPDE3 antibody

Dunce like phosphodiesterase E3 antibody

FLJ97311 antibody

HSPDE4D antibody

PDE43 antibody

PDE4DN2 antibody

Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody

Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila) antibody

Phosphodiesterase 4D cAMP specific antibody

Phosphodiesterase E3 dunce homolog Drosophila antibody

STRK 1 antibody

General Information

Product

PDEB1 Antibody Affinity Purified

Description

Affinity Purified Trypanosoma brucei class 1 phosphodiesterase PDEB1 (PDEB1) Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Trypanosoma brucei

Immunogen

Synthetic MAP peptide taken within amino acid region 1-50 on Trypanososoma brucei class 1 phosphodiesterase PDEB1 protein.

Accession #

GenBank: AY028446.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

Q8WQX9

Overview

Hydrolase, 3',5'-cyclic-nucleotide phosphodiesterase activity

Alternative Nomenclature

Phosphodiesterase 2 C PDEB1 PDE1B Phosphodiesterase B1

General Information

Product

PDE7B Antibody

Description

Affinity Purified cAMP-specific 3',5'-cyclic phosphodiesterase 7B Antibody N-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic cyclic peptide common to all PDE7B variants.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:250

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q9NP56

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in the control of cAMP-mediated neural activity and cAMP metabolism in the brain.

Molecular Function

Hydrolase, 3', 5' -cyclic-AMP phosphodiesterase activity

Subcellular Location

Cytosol

Expression

Highly expressed in brain. Also expressed in heart, liver, skeletal muscle and pancreas.

Alternative Nomenclature

bA472E5.1 antibody

cAMP specific 3 5 cyclic phosphodiesterase 7B antibody

High affinity cAMP specific 3 5 cyclic phosphodiesterase antibody

MGC88256 antibody

PDE 7B antibody

Phosphodiesterase7B antibody

RNPDE7B antibody

Rolipram insensitive phosphodiesterase type 7 antibody

General Information

Product

PDE7B Antibody

Description

Affinity Purified cAMP-specific 3',5'-cyclic phosphodiesterase 7B Antibody

Verified Applications

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic cyclic peptide common to all PDE7B variants.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:250

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q8VIE4

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in the control of cAMP-mediated neural activity and cAMP metabolism in the brain.

Molecular Function

Hydrolase, 3', 5' -cyclic-AMP phosphodiesterase activity

Subcellular Location

Cytosol

Expression

Highly expressed in brain. Also expressed in heart, liver, skeletal muscle and pancreas.

Alternative Nomenclature

bA472E5.1 antibody

cAMP specific 3 5 cyclic phosphodiesterase 7B antibody

High affinity cAMP specific 3 5 cyclic phosphodiesterase antibody

MGC88256 antibody

PDE 7B antibody

Phosphodiesterase7B antibody

RNPDE7B antibody

Rolipram insensitive phosphodiesterase type 7 antibody