General Information

Product

Estrogen Receptor alpha Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide taken within amino acid region 30-80 on human ESTRA protein

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P03372

Overview

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues

Molecular Function

Activator, Receptor

Subcellular Location

Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Nucleus

Expression

Widely expressed. Isoform 3 is not expressed in the pituitary gland

Structure

Belongs to the nuclear hormone receptor family. NR3 subfamily

Alternative Nomenclature

DKFZp686N23123 antibody

ER alpha antibody

ER antibody

Era antibody

ESR antibody

ESR1 antibody

ESRA antibody

Estradiol receptor antibody

Estrogen nuclear receptor alpha antibody

Estrogen receptor 1 antibody

Estrogen receptor antibody

ESTRR antibody

Major ORF antibody

NR3A1 antibody

Nuclear receptor subfamily 3 group A member 1 antibody

General Information

Product

Insulin degrading enzyme Antibody

Verified Applications

ELISA, IP WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 100-150 on human Insulin-degrading enzyme protein.

Accession #

GenBank: EAW50092.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

UPI00002289E9

Overview

Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.

Molecular Function

ATPase activity; Ubiquitin Binding; Zinc ion binding; Insulin binding; Hydrolase; Metalloprotease; Protease

Subcellular Location

Cytoplasm; Cell membrane; Secreted

Structure

Homodimer. Can form higher oligomers. Interacts (via N-terminus) with varicella-zoster virus (VZV) envelope glycoprotein E (via N-terminus); the membrane-associated isoform may function as an entry receptor for this virus.

Alternative Nomenclature

Abeta-degrading protease antibody

FLJ35968 antibody

Ide antibody

insulin protease antibody

Insulin-degrading enzyme antibody

insulinase antibody

insulysin antibody

General Information

Product

FGFR1 Antibody

Description

Affinity Purified Fibroblast Growth Factor Receptor 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide taken within amino acid region 640-690 on human basic fibroblast growth factor receptor protein 1.

Accession #

GenBank: AAA75007.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P11362

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.

Molecular Function

ATP-binding; Fibroblast growth factor binding; Heparin binding; Protein Tyrosine Kinase activity

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm › cytosol. Cytoplasmic vesicle

Expression

Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.

Structure

Monomer. Homodimer after ligand binding. Interacts predominantly with FGF1 and FGF2, but can also interact with FGF3, FGF4, FGF5, FGF6, FGF8, FGF10, FGF19, FGF21, FGF22 and FGF23 (in vitro). Ligand specificity is determined by tissue-specific expression of isoforms, and differences in the third Ig-like domain are crucial for ligand specificity. Affinity for fibroblast growth factors (FGFs) is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Likewise, KLB increases the affinity for FGF19, FGF21 and FGF23. Interacts (phosphorylated on Tyr-766) with PLCG1 (via SH2 domains). Interacts with FRS2. Interacts (via C-terminus) with NEDD4 (via WW3 domain). Interacts with KL (By similarity). Interacts with SHB (via SH2 domain) and GRB10. Interacts with KAL1; this interaction does not interfere with FGF2-binding to FGFR1, but prevents binding of heparin-bound FGF2. Interacts with SOX2 and SOX3.

Alternative Nomenclature

Basic fibroblast growth factor receptor 1 antibody

bFGF R antibody

C FGR antibody

CD 331 antibody

CEK antibody

FGFBR antibody

FGFR 1 antibody

Fibroblast growth factor receptor 1 antibody

FLG antibody

FLG protein antibody

FLT 2 antibody

FMS like tyrosine kinase 2 antibody

Fms related tyrosine kinase 2 Pfeiffer syndrome antibody

Fms-like tyrosine kinase 2 antibody

HBGFR antibody

Heparin binding growth factor receptor antibody

HH2 antibody

HRTFDS antibody

Hydroxyaryl protein kinase antibody

KAL 2 antibody

MFR antibody

N sam tyrosine kinase antibody

N-SAM antibody

OGD antibody

Proto-oncogene c-Fgr antibody

Tyrosylprotein kinase antibody

General Information

Product

Phospho-FGFR1 Antibody

Description

Affinity Purified Phospho-Fibroblast Growth Factor Receptor 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide taken within amino acid region 640-690 on human basic fibroblast growth factor receptor protein 1.

Accession #

GenBank: AAA75007.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P11362

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.

Molecular Function

ATP-binding; Fibroblast growth factor binding; Heparin binding; Protein Tyrosine Kinase activity

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm › cytosol. Cytoplasmic vesicle

Expression

Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.

Structure

Monomer. Homodimer after ligand binding. Interacts predominantly with FGF1 and FGF2, but can also interact with FGF3, FGF4, FGF5, FGF6, FGF8, FGF10, FGF19, FGF21, FGF22 and FGF23 (in vitro). Ligand specificity is determined by tissue-specific expression of isoforms, and differences in the third Ig-like domain are crucial for ligand specificity. Affinity for fibroblast growth factors (FGFs) is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Likewise, KLB increases the affinity for FGF19, FGF21 and FGF23. Interacts (phosphorylated on Tyr-766) with PLCG1 (via SH2 domains). Interacts with FRS2. Interacts (via C-terminus) with NEDD4 (via WW3 domain). Interacts with KL (By similarity). Interacts with SHB (via SH2 domain) and GRB10. Interacts with KAL1; this interaction does not interfere with FGF2-binding to FGFR1, but prevents binding of heparin-bound FGF2. Interacts with SOX2 and SOX3.

Alternative Nomenclature

Basic fibroblast growth factor receptor 1 antibody

bFGF R antibody

C FGR antibody

CD 331 antibody

CEK antibody

FGFBR antibody

FGFR 1 antibody

Fibroblast growth factor receptor 1 antibody

FLG antibody

FLG protein antibody

FLT 2 antibody

FMS like tyrosine kinase 2 antibody

Fms related tyrosine kinase 2 Pfeiffer syndrome antibody

Fms-like tyrosine kinase 2 antibody

HBGFR antibody

Heparin binding growth factor receptor antibody

HH2 antibody

HRTFDS antibody

Hydroxyaryl protein kinase antibody

KAL 2 antibody

MFR antibody

N sam tyrosine kinase antibody

N-SAM antibody

OGD antibody

Proto-oncogene c-Fgr antibody

Tyrosylprotein kinase antibody

General Information

Product

IL12 p40 Antibody

Description

Affinity Purified Interleukin-12 subunit beta precursor Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region spanning 280-335 on rat Interleukin-12 subunit beta protein.

Accession #

NCBI:  NP_072133.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

ffinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9R278

Overview

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Molecular Function

Cytokine receptor activity, protein heterodimerization activity, interleukin-12 alpha subunit binding

Alternative Nomenclature

40 kD Subunit antibody

CLMF 2 antibody

CLMF antibody

Cytotoxic lymphocyte maturation factor 2 antibody

Cytotoxic lymphocyte maturation factor 2 p40 antibody

HGNC:5970 antibody

IL 12 subunit p40 antibody

IL 12B antibody

Interleukin 12 beta chain antibody

Interleukin 12 p40 antibody

Natural killer cell stimulatory factor 2 antibody

NKSF 2 antibody

NKSF antibody

p40 antibody

TcMF antibody

TSF antibody

General Information

Product

ILK Antibody

Description

Affinity Purified Integrin-linked protein kinase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-452 on human Integrin-linked protein kinase.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.66-0.87 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q13418

Overview

Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway.

Molecular Function

ATP binding, protein kinase binding, protein serine/threonine kinase activity, signal transducer activity

Subcellular Location

Cell junction, cell membrane, peripheral membrane protein, cytoplasmic side, cell projection

Expression

Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.

Alternative Nomenclature

59 kDa serine/threonine protein kinase antibody

DKFZp686F1765 antibody

ILK 1 antibody

ILK 2 antibody

ILK antibody

Integrin linked kinase 2 antibody

Integrin-linked protein kinase antibody

p59 antibody

p59ILK antibody

General Information

Product

FNDC5 Antibody

Description

Affinity Purified Fibronectin type III domain-containing protein 5 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequences on human FNDC5 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8K4Z2

Overview

Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA.

Molecular Function

Hormone

Subcellular Location

Cell membrane, Membrane, Peroxisome, Secreted

Expression

highly expressed in skeletal muscle, heart and brain.

Alternative Nomenclature

Fibronectin type III domain containing 5 antibody

FNDC 5 antibody

FRCP2 antibody

irisin antibody

General Information

Product

Lyn Antibody

Description

Affinity Purified Tyrosine-protein kinase Lyn Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Immunogen

Synthetic peptide taken within amino acid region 425-525 on human Lyn protein.

Accession #

NCBI:  NP_002341

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P07948

Overview

Non-receptor tyrosine-protein kinase that plays role in regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents

Molecular Function

Kinase, Transferase, Tyrosine-protein kinase

Subcellular Location

Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Nucleus

Expression

monocytes,placenta,fetal brain, lung, liver,kidney and epidermoid, hematopoietic, and neuronal cells,also primary neuroblastoma tumors

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily

Alternative Nomenclature

AA407514 antibody

EC 2.7.10.2 antibody

FLJ26625 antibody

Hck 2 antibody

JTK 8 antibody

Lck/Yes related novel protein tyrosine kinase antibody

LYN antibody

ONCOGENE LYN antibody

p53Lyn antibody

p56Lyn antibody

Tyrosine protein kinase LYN antibody

V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody

General Information

Product

MIG7 Antibody Affinity Purified

Description

Affinity Purified MIG7 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 170-220 on MIG7 protein.

Accession #

GenBank: ABF71424.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q1AHR6

Alternative Nomenclature

mig 7 antibody

General Information

Product

MRP1 Antibody

Description

Affinity Purified Multidrug resistance-associated protein 1 Antibody

Verified Applications

ELISA, IP, IHC, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequences on rat multidrug resistance-associated protein 1.

Accession #

NCBI:  NP_071617.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:20,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8CG09

Overview

Mediates export of organic anions and drugs from the cytoplasm.

Molecular Function

Hydrolase

Subcellular Location

Cell membrane, Membrane

Expression

Skeletal muscle, brain, heart, spleen, lung and kidney.

Structure

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.

Alternative Nomenclature

ABC29 antibody

ABCC antibody

ABCC1 antibody

ATP binding cassette sub family C (CFTR/MRP) member 1 antibody

ATP binding cassette sub-family C member 1 antibody

DKFZ antibody