PDE4D5 Positive Control

Product no.: PC-PD4D5

$220.00

Description
Publications

General Information
Product	PDE4D5 Positive Control
Description	Western bot positi cAMP-specific phosphodiesterase PDE4D5
Verified Applications	Western blot
Accession #	GenBank: AAC00069.1
Physical Properties
Quantity	5 applications
Volume	Inquire
Form	Wester blot positive control
Concentration	The concentration of the sample is empirically set so that 20 µl per lane in Western and blotted with corresponding affinity purified antibody will give a clean band with a high signal to noise ratio on the gel.
Storage	-20⁰C for long term storage

Protein
Uniprot #	Q08499
Overview	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Molecular Function	Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding
Subcellular Location	Apical cell membrane, Cytoplasm, Membrane
Expression	Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
Structure	Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphotase 1 (PP1) (By similarity). Isoform 5, isoform N3 and isoform 12 bind GNB2L1 via their unique N-terminus. Binds ARRB2. Interacts (via N-terminal region) with SHANK2 (via proline-rich region); the interaction is increased in a PKA-dependent manner.
Alternative Nomenclature	cAMP-specific 3'5'-cyclic phosphodiesterase 4D antibody DPDE3 antibody dunce-like phosphodiesterase E3 antibody hPDE4D5 antibody PDE4D antibody PDE4DN2 antibody Phosphodiesterase 4D antibody Phosphodiesterase 4D cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E3) antibody Phosphodiesterase 4D cAMP-specific antibody STRK1 antibody

Protein information supplied by UniProt

Fabgennix PDE4D5 Antibody has been used in 6 Publications

Wang, Zhen-Zhen, et al. "Phosphodiesterase-4D Knock-down in the Prefrontal Cortex Alleviates Chronic Unpredictable Stress-Induced Depressive-Like Behaviors and Memory Deficits in Mice." Scientific Reports 5 (2015). PMID26161529

Western Blot; Mouse

Mahavadi, Sunila, et al. "Cytokine-induced iNOS and ERK1/2 inhibit adenylyl cyclase type 5/6 activity and stimulate phosphodiesterase 4D5 activity in intestinal longitudinal smooth muscle." American Journal of Physiology-Cell Physiology 307.4 (2014): C402-C411. PMID24944202

Western Blot; Mouse

Li, Yun-Feng et al. “Phosphodiesterase-4D Knockout and RNAi-Mediated Knockdown Enhance Memory and Increase Hippocampal Neurogenesis via Increased cAMP Signaling.” The Journal of neuroscience : the official journal of the Society for Neuroscience 31.1 (2011): 172–183. PMC3079568

Immunohistochemistry, Western Blot; Mouse

Nino, Gustavo et al. “Mechanism Regulating Proasthmatic Effects of Prolonged Homologous Β2-Adrenergic Receptor Desensitization in Airway Smooth Muscle.” American Journal of Physiology - Lung Cellular and Molecular Physiology 297.4 (2009): L746–L757. PMC2770790

Western Blot; Rabbit

Murthy, Karnam S., and Wimolpak Sriwai. "Stimulatory phosphorylation of cAMP-specific PDE4D5 by contractile agonists is mediated by PKC-dependent inactivation of protein phosphatase 2A." American Journal of Physiology-Gastrointestinal and Liver Physiology 294.1 (2008): G327-G335. PMID18006600

Immunoprecipitation, Western Blot; Human

Hajjhussein, Hassan et al. “Changes in NMDA Receptor-Induced Cyclic Nucleotide Synthesis Regulate the Age-Dependent Increase in PDE4A Expression in Primary Cortical Cultures.” Brain research 1149 (2007): 58–68. PMC2720317

Immunoprecipitation, Western Blot; Human

Publishing research using PD4D5-451AP? Please let us know, we can cite the reference in this datasheet.