General Information

Product

BST2 Antibody

Description

Affinity Purified Bonemarrow stromal antigen 2 Antibody Mid-Region

Verified Applications

ELISA, CM, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Mid-Region Epitope Synthetic peptide taken within amino acid region 50-100 on human Bonemarrow stromal antigen 2 protein.

Accession #

GenBank:  BAD96844

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:2,000

ELISA

1:2,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q10589

Overview

IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells.

Molecular Function

Antiviral defense; B-cell activation; Immunity; Innate immunity

Subcellular Location

Gogli apparatus, trans-golgi network; Cell membrane, Single-pass type II membrane protein. Lipid anchor, GPI anchor; Late endosome; Membrane raft; Cytoplasm; Apical cell membrane

Expression

Liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal msucle and brain. Overexpressed in multiple myeloma cells. B-cells, T-cells, monocytes, NK cells and dendritic cells. Induced by type I interforns. Down regulated by viral antagonistic factors.

Structure

Parallel homodimer; disulfide linked. Cytoplasmic domain interacts with ARHGAP44 and MMP14.

Alternative Nomenclature

Bone marrow stromal antigen 2 antibody

Bone marrow stromal cell antigen 2 antibody

BST 2 antibody

CD 317 antibody

CD317 antigen antibody

HM1.24 antigen antibody

NPC A 7 antibody

Tetherin antibody

General Information

Product

BST2 Antibody

Description

Affinity Purified Bonemarrow stromal antigen 2 Antibody C-epitope

Verified Applications

ELISA, CM, ICC, IF, IHC, IP, WB

Host

Rabbit

Immunogen

C-epitope Synthetic peptide taken within amino acid region 100-150 on human Bonemarrow stromal antigen 2 protein.

Accession #

GenBank:  BAD96844

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DOT Blot

1:2,000

ELISA

1:2,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q10589

Overview

IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells.

Molecular Function

Antiviral defense; B-cell activation; Immunity; Innate immunity

Subcellular Location

Gogli apparatus, trans-golgi network; Cell membrane, Single-pass type II membrane protein. Lipid anchor, GPI anchor; Late endosome; Membrane raft; Cytoplasm; Apical cell membrane

Expression

Liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal msucle and brain. Overexpressed in multiple myeloma cells. B-cells, T-cells, monocytes, NK cells and dendritic cells. Induced by type I interforns. Down regulated by viral antagonistic factors.

Structure

Parallel homodimer; disulfide linked. Cytoplasmic domain interacts with ARHGAP44 and MMP14.

Alternative Nomenclature

Bone marrow stromal antigen 2 antibody

Bone marrow stromal cell antigen 2 antibody

BST 2 antibody

CD 317 antibody

CD317 antigen antibody

HM1.24 antigen antibody

NPC A 7 antibody

Tetherin antibody

General Information

Product

PODXL Antibody

Description

Affinity Purified Pan Podocalyxin Antibody

Verified Applications

DB, ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

C-epitope synthetic peptide taken within amino acid region 510-560 on human PODXL protein.

Accession #

NCBI:  NP_001018121.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O00592

Overview

Involved in the regulation of both adhesion and cell morphology and cancer progression.  Funciton as an anti-aging adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion.

Subcellular Location

Apical cell membrane, Cell projection, Membrane

Expression

Glomerular epithelium cell (podocyte)

Structure

Monomer; when associated with the membrane raft.  Oligomer; when integrated in the apical membrane.

Alternative Nomenclature

GCTM-2 antigen antibody

Gp200 antibody

MGC138240 antibody

PC antibody

PCLP antibody

PCLP1 antibody

Pcx antibody

Podocalyxin antibody

Podocalyxin-like protein 1 antibody

Podxl antibody

General Information

Product

PODXL1 Antibody

Description

Affinity Purified Podocalyxin isoform 1 Antibody N-epitope

Verified Applications

DB, ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide corresponding amino acid region 200-250 on human PODXL protein.

Accession #

NCBI:  NP_001018121.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O00592

Overview

Involved in the regulation of both adhesion and cell morphology and cancer progression.  Funciton as an anti-aging adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion.

Subcellular Location

Apical cell membrane, Cell projection, Membrane

Expression

Glomerular epithelium cell (podocyte)

Structure

Monomer; when associated with the membrane raft.  Oligomer; when integrated in the apical membrane.

Alternative Nomenclature

GCTM-2 antigen antibody

Gp200 antibody

MGC138240 antibody

PC antibody

PCLP antibody

PCLP1 antibody

Pcx antibody

Podocalyxin antibody

Podocalyxin-like protein 1 antibody

Podxl antibody

General Information

Product

SGK1 Antibody

Description

Affinity Purified Serine/Threonine-protein Kinase Sgk1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide taken within amino acid region 40-90 on human SGK1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O00141

Overview

Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cellular enzymes, transcription factors, neuronal excitability, cell growth, proliferation, survival, migration and apoptosis. Plays an important role in cellular stress response. Contributes to regulation of renal Na+ retention, renal K+ elimination, salt appetite, gastric acid secretion, intestinal Na+/H+ exchange and nutrient transport, insulin-dependent salt sensitivity of blood pressure, salt sensitivity of peripheral glucose uptake, cardiac repolarization and memory consolidation. Up-regulates Na+ channels: SCNN1A/ENAC, SCN5A and ASIC1/ACCN2, K+ channels: KCNJ1/ROMK1, KCNA1-5, KCNQ1-5 and KCNE1, epithelial Ca2+ channels: TRPV5 and TRPV6, chloride channels: BSND, CLCN2 and CFTR, glutamate transporters: SLC1A3/EAAT1, SLC1A2 /EAAT2, SLC1A1/EAAT3, SLC1A6/EAAT4 and SLC1A7/EAAT5, amino acid transporters: SLC1A5/ASCT2, SLC38A1/SN1 and SLC6A19, creatine transporter: SLC6A8, Na+/dicarboxylate cotransporter: SLC13A2/NADC1, Na+-dependent phosphate cotransporter: SLC34A2/NAPI-2B, glutamate receptor: GRIK2/GLUR6. Up-regulates carriers: SLC9A3/NHE3, SLC12A1/NKCC2, SLC12A3/NCC, SLC5A3/SMIT, SLC2A1/GLUT1, SLC5A1/SGLT1 and SLC15A2/PEPT2. Regulates enzymes: GSK3A/B, PMM2 and Na+/K+ ATPase, and transcription factors: CTNNB1 and nuclear factor NF-kappa-B. Stimulates sodium transport into epithelial cells by enhancing the stability and expression of SCNN1A/ENAC. This is achieved by phosphorylating the NEDD4L ubiquitin E3 ligase, promoting its interaction with 14-3-3 proteins, thereby preventing it from binding to SCNN1A/ENAC and targeting it for degradation. Regulates store-operated Ca(+2) entry (SOCE) by stimulating ORAI1 and STIM1.

Molecular Function

ATP-binding, Nucleotide binding; Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm; Nucleus; Endoplasmic reticulum membrane; Cell membrane; Mitochondrion

Expression

Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.

Structure

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 protein kinase domain.

Alternative Nomenclature

OTTHUMP00000017247 antibody
Serine/threonine protein kinase Sgk1 antibody
Serum and glucocorticoid regulated kinase antibody
Serum/glucocorticoid-regulated kinase 1 antibody
SGK 1 antibody
SGK antibody
Sgk1 variant i3 antibody

General Information

Product

BMPR1A Antibody

Description

Affinity Purified Bone morphogenetic protein receptor type-1A Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 460-510 on human Bone morphogenetic protein receptor type-1A.

Accession #

NCBI:  NP_004320

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P36894

Overview

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP2, BMP4, GDF5 and GDF6. Positively regulates chondrocyte differentiation through GDF5 interaction. Mediates induction of adipogenesis by GDF6.

Molecular Function

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Highly expressed in skeletal muscle.

Structure

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.

Contains 1 GS domain.

Contains 1 protein kinase domain.

Alternative Nomenclature

Activin A receptor type II like kinase 3 

Activin receptor like kinase 3 

ACVRLK 3

ALK 3 

BMP type-1A receptor 

BMPR 1A 

Bmpr 

Bone morphogenetic protein receptor type-1A

BR1a

CD292 

Serine threonine protein kinase receptor R5

SKR5 

zBMPR IA

General Information

Product

DNER Antibody

Description

Affinity Purified Delta and Notch-like Epidermal growth factor-related receptor precursor Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 700-737 on human Delta and Notch-like Epidermal growth factor-related receptorprotein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q8NFT8

Overview

Activator of the NOTCH1 pathway. May mediate neuron-glia interaction during astrocytogenesis.

Molecular Function

calcium ion binding

clathrin binding

Notch binding

transmembrane signaling receptor activity

Subcellular Location

Plasma Membrane

Expression

Expressed in brain, spinal cord and adrenal gland.

Structure

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Alternative Nomenclature

bet antibody

Brain EGF repeat-containing transmembrane protein antibody

Bret antibody

Delta and Notch-like epidermal growth factor-related receptor antibody

Delta notch like EGF repeat containing transmembrane antibody

Dner antibody

PRO299 antibody

Transmembrane protein Bet antibody

UNQ26 antibody

General Information

Product

CD45 Antibody

Description

Affinity Purified Receptor-type tyrosine-protein phosphatase C Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 1100-1150 on human Receptor-type tyrosine-protein phosphatase C.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P08575

Subcellular Location

Cell membrane, Membrane

Structure

Repeat, Signal, Transmembrane, Transmembrane helix

Alternative Nomenclature

B220 antibody
CD45 antigen antibody
CD45R antibody
GP180 antibody
LCA antibody
Leukocyte common antigen antibody
loc antibody
Ly-5 antibody
LY5 antibody
Lyt-4 antibody
Protein tyrosine phosphatase receptor type c polypeptide antibody
Ptprc antibody
Receptor-type tyrosine-protein phosphatase C antibody
T200 antibody
T200 glycoprotein antibody
T200 leukocyte common antigen antibody