General Information |
|
Product |
Phospho PDE5A Antibody FITC |
Description |
FITC-Conjugated Phosphorylated Phosphodiesterase 5A Antibody |
Verified Applications |
ELISA, ICC, IHC, IP, WB |
Host |
Rabbit |
Species Cross Reactivity |
Bovine, Human, Mouse, Pig, Rat, Sheep |
Immunogen |
Phosphorylated synthetic peptide common to all PDE5A variants taken within amino acid region 120-170 on human PDE5A protein. |
Specificity | This antibody will detect only PDE5A protein that has been phosphorylated at the PKG site. Will not detect unphosphorylated PDE5A, or other PDE family members. |
Accession # |
GenBank: EAX05277.1 |
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|
Quantity |
100 µg |
Volume |
200 µl |
Concentration |
0.5 µg/µl in antibody stabilization buffer |
Form |
FITC-Conjugated |
Clonality |
Polyclonal |
Storage |
-20⁰C for long term storage |
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|
Confocal Microscopy |
1:100 |
DB |
1:10,000 |
ELISA |
1:10,000 |
Immunocytochemistry |
1:100 |
Immunofluorescence |
1:100 |
Immunohistochemistry |
1:100 |
Immunoprecipitation |
1:200 |
Western Blot |
1:500 |
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Uniprot # |
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Overview |
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. |
Molecular Function |
Hydrolase, 3',5'-cyclic-GMP phosphodiesterase activity, cGMP binding |
Subcellular Location |
cytosol |
Expression |
Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung. |
Alternative Nomenclature |
5''-cyclic phosphodiesterase antibody CGB PDE antibody cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody cGMP binding/cGMP specific phosphodiesterase antibody cGMP specific 3'5' cyclic phosphodiesterase antibody cGMP specific phosphodiesterase PDE5A2 antibody cGMP specific phosphodiesterase type 5A antibody cGMP-binding cGMP-specific phosphodiesterase antibody cGMP-specific 3'' antibody CN5A antibody CN5N antibody PDE 5 antibody PDE 5A antibody PDE5A1 antibody Phosphodiesterase 5A antibody Phosphodiesterase 5A cGMP specific antibody Phosphodiesterase isozyme 5 antibody |
Protein information supplied by UniProt
Fabgennix Phospho-PDE5A Antibody has been used in 14 Publications |
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MHerrera, Emilio A., et al. "The role of nitric oxide signaling in pulmonary circulation of highand low-altitude newborn sheep under basal and acute hypoxic conditions." Nitric Oxide, 89, 71-80. PMID: 31063821 |
Western Blot; Sheep |
Musicki, Biljana, et al. "Testosterone replacement in transgenic sickle cell mice controls priapic activity and upregulates PDE5 expression and eNOS activity in the penis." Andrology 6.1 (2018): 184-191. PMID29145710
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Western Blot; Mouse |
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Western Blot; Mouse |
Nino, Paula Katherina Bautista, et al. "Phosphodiesterase 1 regulation is a key mechanism in vascular aging." Clinical Science (2015): CS20140753. PMID26464516 |
Western Blot; Mouse |
Nino, Paula Katherina Bautista, et al. "Phosphodiesterase 1 regulation is a key mechanism in vascular aging." Clinical Science (2015): CS20140753. PMID26464516
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Western Blot; Mouse |
Rajagopal, Senthilkumar, et al. "Cytokine-Induced S-Nitrosylation of Soluble Guanylyl Cyclase and Expression of Phosphodiesterase 1A Contribute to Dysfunction of Longitudinal Smooth Muscle Relaxation." Journal of Pharmacology and Experimental Therapeutics 352.3 (2015): 509-518.PMID25550199
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Western Blot; Mouse |
Shukla, Praveen, Chengwen Sun, and Stephen T. O’Rourke. “Melatonin Inhibits Nitric Oxide Signaling by Increasing PDE5 Phosphorylation in Coronary Arteries.” American Journal of Physiology - Heart and Circulatory Physiology 303.12 (2012): H1418–H1425. PMC3532536
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Western Blot; Pig |
Farrow, Kathryn N. et al. “Brief Hyperoxia Increases Mitochondrial Oxidation and Increases Phosphodiesterase 5 Activity in Fetal Pulmonary Artery Smooth Muscle Cells.” Antioxidants & Redox Signaling 17.3 (2012): 460–470. PMC3365357
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Western Blot; Mouse |
Luong, Christina, et al. "Antenatal sildenafil treatment attenuates pulmonary hypertension in experimental congenital diaphragmatic hernia." Circulation 123.19 (2011): 2120-2131. PMID21537000
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Western Blot; Rat |
Mokni, Walid et al. “Concerted Regulation of cGMP and cAMP Phosphodiesterases in Early Cardiac Hypertrophy Induced by Angiotensin II.” Ed. Karl-Wilhelm Koch. PLoS ONE 5.12 (2010): e14227. PMC2997062
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Immunohistochemistry, Western Blot; Rat |
Murthy, K S. “Contractile Agonists Attenuate cGMP Levels by Stimulating Phosphorylation of cGMP-Specific PDE5; an Effect Mediated by RhoA/PKC-Dependent Inhibition of Protein Phosphatase 1.” British Journal of Pharmacology 153.6 (2008): 1214–1224. PMC2275441
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Western Blot; Rabbit |
Wilson, Lindsay S. et al. “Compartmentation and Compartment-Specific Regulation of PDE5 by Protein Kinase G Allows Selective cGMP-Mediated Regulation of Platelet Functions.” Proceedings of the National Academy of Sciences of the United States of America 105.36 (2008) PMC2533244
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Western Blot; Human |
Monfort, Pilar, María‐Dolores Muñoz, and Vicente Felipo. "Chronic hyperammonemia in vivo impairs long‐term potentiation in hippocampus by altering activation of cyclic GMP‐dependent‐protein kinase and of phosphodiesterase 5." Journal of neurochemistry 94.4 (2005): 934-942. PMID16092938
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Western Blot; Rat |
Champion, Hunter C. et al. “Phosphodiesterase-5A Dysregulation in Penile Erectile Tissue Is a Mechanism of Priapism.” Proceedings of the National Academy of Sciences of the United States of America 102.5 (2005): 1661–1666. PMC547836
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Western Blot; Mouse |
Publishing research using PPD5A-140AP? Please let us know
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