General Information

Product

Progesterone Receptor Antibody

Description

Affinity Purified Progesterone Receptor Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 350-400 on human Progesterone receptor protein.

Accession #

GenBank: AAD01587.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P06401

Overview

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

Molecular Function

DNA binding; Enzyme binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; Steriod hormone receptor activity; Transcription regulation

Subcellular Location

Nucleus. Cytoplasm
Note: Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G1 and G2/M phases.
Isoform A : Nucleus. Cytoplasm
Note: Mainly nuclear.
Isoform 4 : Mitochondrion outer membrane
mitochondrial outer membrane
Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

Structure

Interacts with SMARD1 and UNC45A. Interacts with CUEDC2; the interaction promotes ubiquitination, decreases sumoylation, and repesses transcriptional activity. Interacts with PIAS3; the interaction promotes sumoylation of PR in a hormone-dependent manner, inhibits DNA-binding, and alters nuclear export. Interacts with SP1; the interaction requires ligand-induced phosphorylation on Ser-345 by ERK1/2 MAPK. Interacts with PRMT2.

Alternative Nomenclature

NR3C3 antibody

Nuclear receptor subfamily 3 group C member 3 antibody

PGR antibody

PR antibody

PR antibody

PRA antibody

PRB antibody

Progesterone receptor antibody

Progestin receptor form A antibody

Progestin receptor form B antibody

Progestin receptor form C antibody

General Information

Product

Progesterone Receptor Antibody

Description

Affinity Purified Progesterone Receptor Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 320-370 on human Progesterone receptor protein.

Accession #

GenBank: AAD01587.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P06401

Overview

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

Molecular Function

DNA binding; Enzyme binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; Steriod hormone receptor activity; Transcription regulation

Subcellular Location

Nucleus. Cytoplasm
Note: Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G1 and G2/M phases.
Isoform A : Nucleus. Cytoplasm
Note: Mainly nuclear.
Isoform 4 : Mitochondrion outer membrane
mitochondrial outer membrane
Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

Structure

Interacts with SMARD1 and UNC45A. Interacts with CUEDC2; the interaction promotes ubiquitination, decreases sumoylation, and repesses transcriptional activity. Interacts with PIAS3; the interaction promotes sumoylation of PR in a hormone-dependent manner, inhibits DNA-binding, and alters nuclear export. Interacts with SP1; the interaction requires ligand-induced phosphorylation on Ser-345 by ERK1/2 MAPK. Interacts with PRMT2.

Alternative Nomenclature

NR3C3 antibody

Nuclear receptor subfamily 3 group C member 3 antibody

PGR antibody

PR antibody

PR antibody

PRA antibody

PRB antibody

Progesterone receptor antibody

Progestin receptor form A antibody

Progestin receptor form B antibody

Progestin receptor form C antibody

General Information

Product

Calretinin Antibody

Description

Affinity Purified Calretinin Antibody N-epitope

Verified Applications

CM, DB, ELISA, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human Calretinin protein.

Accession #

GenBank: CAA39991.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:50-1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:50-1:200

Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P22676

Overview

Calcium binding protein which is abundant in auditory neurons.

Molecular Function

Calcium ion binding; Metal binding

Subcellular Location

Cytoplasm; gap junction; nucleus

Expression

Brain

Structure

Belongs to calbindin family.  Has 6 EF-hand domains.

Alternative Nomenclature

29 kDa calbindin antibody

CAB29 antibody

CAL2 antibody

CALB2 antibody

Calbindin 2 29kDa antibody

Calbindin 2 antibody

Calbindin D29K antibody

Calbindin2 antibody

Calretinin antibody

CR antibody

General Information

Product

Calretinin Antibody

Verified Applications

CM, DB, ELISA, IF, IHC-P, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within amino acid region 220-271. Conjugated to Keyhole Limpet Haemocyanin (KLH). Peptide was covalently modified.

Sequence:

L DALLKDLYEK NKKEMNIQQL TNYRKSVMSL AEGKLYRKD LEIVLCSEPP M

Accession #

GenBank: CAA39991.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:200

Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200

Western Blot

1:500. Predicted molecular weight: 32 kDa

Protein

Uniprot #

P22676

Overview

Calcium binding protein which is abundant in auditory neurons.

Molecular Function

Calcium ion binding; Metal binding

Subcellular Location

Cytoplasm; gap junction; nucleus

Expression

Brain

Structure

Belongs to calbindin family.  Has 6 EF-hand domains.

Alternative Nomenclature

29 kDa calbindin antibody

CAB29 antibody

CAL2 antibody

CALB2 antibody

Calbindin 2 29kDa antibody

Calbindin 2 antibody

Calbindin D29K antibody

Calbindin2 antibody

Calretinin antibody

CR antibody

General Information

Product

MOR1 Antibody

Description

Affinity Purified Mu-type opioid receptor isoform MOR-1i Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 405-455 on human Mu-type opiod receptor isoform MOR-1i protein.

Accession #

NCBI:  NP_001272453

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P35372

Overview

Receptor for endogenous opioids such as beta-endorphin and endomorphin. Receptor for natural and synthetic opioids including morphine, heroin, DAMGO, fentanyl, etorphine, buprenorphin and methadone. Agonist binding to the receptor induces coupling to an inactive GDP-bound heterotrimeric G-protein complex and subsequent exchange of GDP for GTP in the G-protein alpha subunit leading to dissociation of the G-protein complex with the free GTP-bound G-protein alpha and the G-protein beta-gamma dimer activating downstream cellular effectors.

Molecular Function

G-protein coupled receptor, Receptor, Transducer

Subcellular Location

Cell membrane > Multi-pass membrane protein. Isoform 12: Cytoplasm

Expression

Expressed in brain. Isoform 16 and isoform 17 are detected in brain.

Structure

Forms homooligomers and heterooligomers with other GPCRs, such as OPRD1, OPRK1, OPRL1, NPFFR2, ADRA2A, SSTR2, CNR1 and CCR5 (probably in dimeric forms). Interacts with PPL; the interaction disrupts agonist-mediated G-protein activation. Interacts (via C-terminus) with DNAJB4 (via C-terminus). Interacts with calmodulin; the interaction inhibits the constitutive activity of OPRM1; it abolishes basal and attenuates agonist-stimulated G-protein coupling. Interacts with FLNA, PLD2, RANBP9 and WLS. Interacts with GPM6A, RTP4, SYP, GNAS, RGS9, RGS17, RGS20, RGS4, PPP1R9B and HINT1.

Alternative Nomenclature

hMOP antibody

LMOR antibody

M-OR-1 antibody

MOP antibody

MOR antibody

MOR-1 antibody

Mu opiate receptor antibody

Mu opioid receptor antibody

Opioid receptor mu 1 antibody

OPRM antibody

OPRM1 antibody