General Information

Product

proBNP Antibody

Description

Affinity Purified Gamma-brain natriuretic peptide Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 100-150 on human proBNP.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.8 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P16860

Overview

A cardiac hormone that may also serve as a paracrine antifibrotic factor in the heart.  Also functions in cardiovascular homeostasis via natriuresis, diuresis, vasorelaxation, and the inhibition of renin and aldosterone secretion.

Molecular Function

Diuretic hormone activity, Hormone, Vasoactive

Subcellular Location

Secreted

Expression

Is expressed in the brain and atria, however it is expressed at much lower levels than ANP.

Alternative Nomenclature

BNP 32 antibody

BNP antibody

BNP(1-32) antibody

BNP(5-29) antibody

Brain Natriuretic Peptide 32 antibody

Gamma brain natriuretic peptide antibody

Natriuretic peptide precursor type B antibody

NPPB antibody

General Information

Product

Phospho Ephrin receptor A4 Antibody Affinity Purified

Description

Affinity Purified Phosphorylated Ephrin type-A receptor 4 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 570-630 on human EPHA R4 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q03137

Overview

Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Highest expression in the adult brain and retina and also detectable in kidney, lung, skeletal muscle and thymus. Not detected in heart and liver.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts (phosphorylated at position Tyr-602) with FYN. Interacts (via

Alternative Nomenclature

Cek 8 antibody

EK8 antibody

eph receptor a4 antibody

EPH-like kinase 8 antibody

EphA4 antibody

Ephrin receptor Eph A4 antibody

hEK8 antibody

Receptor protein tyrosine kinase HEK 8 antibody

Sek 1 antibody

SEK antibody

TYRO 1 protein tyrosine kinase antibody

Tyrosine protein kinase receptor SEK antibody

General Information

Product

PINK1 Antibody

Description

Affinity Purified Serine/Threonine-protein kinase PINK1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on human PINK1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9BXM7

Overview

During cellular stress it serves to protect against mitochondrial dysfunction through the phosphorylation of mitochondrial proteins. Uses selective autophagy to clear mitochondria that has been damaged.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Mitochondrion outer membrane; Single-pass membrane protein, Cytoplasm

Expression

Expression is detected at high levels in the heart, skeletal muscles and testis.  Expression is detected at lower levels in the brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine.

Structure

Interacts with PARK2 and FBXO7

Alternative Nomenclature

BRPK antibody

FLJ27236 antibody

mitochondrial antibody

PARK 6 antibody

Phosphatase and Tensin Homolog antibody

PINK 1 antibody

Protein kinase BRPK antibody

PTEN induced putative kinase 1 antibody

Serine/threonine-protein kinase PINK1 antibody

General Information

Product

cGKI Antibody

Description

Affinity Purified cGMP-dependent protein kinase 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 609-659 on human protein kinase G alpha subunit.

Accession #

NCBI:  NP_001091982.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13976

Overview

Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm

Expression

Primarily expressed in lung and placenta

Structure

Isoform alpha: parallel homodimer or heterodimer and also heterotetramer. Interacts directly with PPP1R12A. Non-covalent dimer of dimer of PRKG1-PRKG1 and PPP1R12A-PPP1R12A.

Alternative Nomenclature

cGK 1 antibody

cGKI alpha antibody

CGKI antibody

cGKI beta antibody

cGMP-dependent protein kinase 1 antibody

DKFZp686K042 antibody

FLJ36117 antibody

KGPB antibody

MGC71944 antibody

pkg antibody

PRKG1 antibody

PRKGR1A antibody

PRKGR1B antibody

General Information

Product

Phospho-PDE5A Antibody

Description

Affinity Purified Phosphorylated Phosphodiesterase 5A Antibody

Verified Applications

ELISA, ICC, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Mouse, Pig, Rat, Sheep

Immunogen

Synthetic peptide common to all PDE5A variants taken within amino acid region 120-170 on human PDE5A protein. Phosphoryalted 

Accession #

GenBank: EAX05277.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.0-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

UPI0000EE2B22

Overview

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.

Molecular Function

Hydrolase, 3',5'-cyclic-GMP phosphodiesterase activity, cGMP binding

Subcellular Location

cytosol

Expression

Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.

Alternative Nomenclature

5''-cyclic phosphodiesterase antibody

CGB PDE antibody

cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody

cGMP binding/cGMP specific phosphodiesterase antibody

cGMP specific phosphodiesterase type 5A antibody

CN5A antibody

CN5N antibody

PDE 5 antibody

PDE 5A antibody

PDE5A1 antibody

Phosphodiesterase 5A antibody

Phosphodiesterase 5A cGMP specific antibody

Phosphodiesterase isozyme 5 antibody

General Information

Product

Phospho-STAT3 Antibody

Description

Affinity Purified Phosphorylated Signal transducer and activator of transcription Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Phosphorylated synthetic peptide taken within amino acid region 700-750 on human STAT3 protein.

Accession #

NCBI:  NP_644805

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P40763

Overview

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes.

Molecular Function

Activator, calcium ion binding, DNA binding

Subcellular Location

Cytoplasm. Nucleus

Expression

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Structure

Forms a homodimer or a heterodimer with a related family member (at least STAT1). Interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1 and TMF1. Interacts with HCV core protein. Interacts with IL23R in presence of IL23. Interacts (via SH2 domain) with NLK. Interacts with ARL2BP; the interaction is enhanced by LIF and JAK1 expression (By similarity).

Alternative Nomenclature

Acute Phase Response Factor antibody

APRF antibody

DNA binding protein APRF antibody

HIES antibody

Signal Transductor and Activator of Transcription 3 antibody

General Information

Product

STAT3 Antibody

Description

Affinity Purified Signal transducer and activator of transcription Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 700-750 on human STAT3 protein.

Accession #

NCBI:  NP_644805

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P40763

Overview

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes.

Molecular Function

Activator, calcium ion binding, DNA binding

Subcellular Location

Cytoplasm. Nucleus

Expression

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Structure

Forms a homodimer or a heterodimer with a related family member (at least STAT1). Interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1 and TMF1. Interacts with HCV core protein. Interacts with IL23R in presence of IL23. 

Alternative Nomenclature

Acute Phase Response Factor antibody

APRF antibody

DNA binding protein APRF antibody

HIES antibody

Signal transducer and activator of transcription 3 (acute phase response factor) antibody

STAT 3 antibody

General Information

Product

SFRP2 Antibody 

Description

Affinity Purified Secreted frizzled-related protein 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 225-295 on human SFRP2.

Accession #

GenBank: AAH08666.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q96HF1

Overview

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.

Molecular Function

Endopeptidase activator activity, integrin binding, PDZ domain binding, Wnt-activated receptor activity, fibronectin binding, receptor agonist activity, Wnt-protein binding

Subcellular Location

Secreted

Expression

Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon.

Alternative Nomenclature

AI851596 antibody
Frizzled related protein 2 antibody
FRP2 antibody
SARP1 antibody
SDF5 antibody
Secreted apoptosis related protein 1 antibody
Secreted frizzled-related protein 2 antibody

General Information

Product

Soluble Guanylyl Cyclase alpha 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 654-704 on rat Guanylyl cyclase alpha 1 subunit protein.

Accession #

GenBank: BAC24016.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.7 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8CJD2

Molecular Function

guanylate cyclase activity, heme binding, nucleotide binding

Alternative Nomenclature

GC S alpha 1 antibody

Guanylate cyclase soluble subunit alpha 1 antibody

Gucy1a1 antibody

Soluble guanylate cyclase large subunit antibody

General Information

Product

SIRT5 Antibody

Description

Affinity Purified Sirtuin 5 (silent mating type information regulation 2 homolog) 5 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 260-310 on rat Sirtuin 5 protein.

Accession #

NCBI: NP_001004256.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.66-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q68FX9

Overview

NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro.

Molecular Function

Hydrolase; Metal-binding; NAD; Zinc

Subcellular Location

Isoform 1:  Cytoplasm, Mitochondrion  Isoform 2:  Mitochondrion

Expression

Widely expressed.

Structure

Interacts with CPS1 (By similarity). Monomer. Homodimer. Forms homodimers upon suramin binding.

Alternative Nomenclature

NAD dependent deacetylase sirtuin 5 antibody

Regulatory protein SIR2 homolog 5 antibody

Silent mating type information regulation 2 Sir2 like 5 antibody

SIR2-like protein 5 antibody

SIR2L5 antibody

Sirtuin type 5 antibody