General Information
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Product
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TNIK Antibody
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Description
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Affinity Purified TRAF2 and NCK-interacting protein kinase isoform 1 Antibody
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Verified Applications
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ELISA, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Mouse, Rat
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Immunogen
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Sythetic peptide taken within amino acid region 1250-1300 on human TRAF2 and NCK-interacting protein kinase protein.
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.5-1.5 µg/µl in antibody stabilization buffer
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Immunoglobulin
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IgG
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Form
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Affinity Purified
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Purification Method
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Immobilized antigen affinity chromatography
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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ELISA
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1:50,000
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Immunoprecipitation
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1:200
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Western Blot
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1:2,500-1:5,000
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Protein
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Uniprot #
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Q9UKE5
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Overview
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Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. Phosphorylates SMAD1 on Thr-322.
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Molecular Function
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ATP binding
MAP kinase kinase kinase kinase activity
protein kinase activity
protein serine/threonine kinase activity
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Subcellular Location
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Nucleus, Cytoskeleton, Endosome
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Expression
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Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues.
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Structure
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Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
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Alternative Nomenclature
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EC 2.7.11.1 antibody
KIAA0551 antibody
Tnik antibody
TNIK_HUMAN antibody
Traf2 and NCK interacting kinase antibody
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