General Information

Product

Axl Antibody

Description

Affinity Purified AXL receptor tyrosine kinase UFO isoform 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 840-880 on mouse AXL protein. The peptide was post translationally modified to acheive proper orientation and antigenicity.

Accession #

NCBI:  NP_033491

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Western Blot

1:500

Protein

Uniprot #

Q00993

Overview

Receptor tyrosine kinase that transduces signals from the ECM into cytoplasm by binding growth factor GAS6. Regulates survival, proliferation, migration and differentiation.

Molecular Function

ATP-binding, Nucleotide binding, Kinase, Receptor, Transferase, Tyrosine-protein kinase, Immunity Differentation and Innate Immunity.

Subcellular Location

Cell membrane; Single-pass type II membrane protein.

Expression

Developing tissues in late embryogenesis. In cells forming organ capsules as well as in connective tissue structures in adult.

Structure

Heterodimer and heterotetramer with ligand GAS6.

Alternative Nomenclature

Adhesion related kinase 

Ark 

Tyro7 

Tyrosine protein kinase receptor UFO 

General Information

Product

CCK4 / PTK7 Antibody

Description

Affinity Purified Protein Tyrosine Kinase 7 Antibody

Verified Applications

ELISA, ICC/IF, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Purified Protein tyrosine kinase 7 protein was emulsified in complete adjuvant and used to immunize the rabbits. 

Accession #

NCBI:  AAH50050

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal. The anti-serum was pre-cleared for tag protein and then affinity purified on immobilized PTK7 affinity chromatography.

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q12852

Overview

Activator of JNK/SAPK pathway.  Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.

Molecular Function

ATP-binding, magnesium, Nucleotide-binding.

Subcellular Location

Cytoplasm, Membrane, Peripheral membrane protein

Expression

Highly expressed in brain and kidney.

Structure

Interacts with MBIP.

Alternative Nomenclature

Colon carcinoma kinase 4 antibody

Inactive tyrosine-protein kinase 7 antibody

mPTK7/CCK4 antibody

Serum response factor antibody

Srf antibody

General Information

Product

ErbB 2 Antibody

Description

Affinity Purified Receptor tyrosine-protein kinase erB-2 isoform b Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 130-180 receptor tyrosine-protein kinase erbB-2 protein.

Accession #

NCBI:  NP_001005862.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P04626

Overview

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs).

Molecular Function

Activator, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein, Cytoplasm>perinuclear region, Nucleus. Isoform 2: Cytoplasm, Nucleus. Isoform 3: Cytoplasm, Nucleus.

Expression

Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

Structure

Homodimer. Heterodimer with EGFR, ERBB3 and ERBB4. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with PRKCABP and PLXNB1. Interacts (when phosphorylated on Tyr-1248) with MEMO1. Interacts with MUC1; the interaction is enhanced by hereguline (HRG).

Alternative Nomenclature

Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody

c erb B2 antibody

CD340 antibody

cneu antibody

Erb B2 antibody

Erythroblastosis Virus antibody

HER 2 antibody

Herstatin antibody

Metastatic lymph node gene 19 protein antibody

MLN 19 antibody

NEU proto oncogene antibody

Neuro Glioblastoma Derived Oncogene Homolog antibody

NGL antibody

p185 ErbB2 antibody

Proto-oncogene Neu antibody

Receptor Protein Tyrosine Kinase ErbB2 Precursor antibody

TKR1 antibody

verbb2 antibody

General Information

Product

ErbB 2 Antibody

Description

Affinity Purified Receptor tyrosine-protein kinase erB-2 isoform a Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Predicted: Rat

Immunogen

Synthetic peptide taken within amino acid region 1125-1175 receptor tyrosine-protein kinase erbB-2 protein.

Accession #

NCBI:  NP_004439.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-1.25 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Determinant

N-epitope

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P04626

Overview

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs).

Molecular Function

Activator, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein, Cytoplasm>perinuclear region, Nucleus. Isoform 2: Cytoplasm, Nucleus. Isoform 3: Cytoplasm, Nucleus.

Expression

Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

Structure

Homodimer. Heterodimer with EGFR, ERBB3 and ERBB4. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with PRKCABP and PLXNB1. Interacts (when phosphorylated on Tyr-1248) with MEMO1. Interacts with MUC1; the interaction is enhanced by heregulin (HRG).

Alternative Nomenclature

Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody

CD340 antibody

CerbB2 antibody

cneu antibody

Erb B2 antibody

erbb2 antibody

Erythroblastosis Virus antibody

HER 2 antibody

Herstatin antibody

Metastatic lymph node gene 19 protein antibody

MLN 19 antibody

NEU antibody

Neuro Glioblastoma Derived Oncogene Homolog antibody

NGL antibody

p185 ErbB2 antibody

Proto-oncogene Neu antibody

Receptor Protein Tyrosine Kinase ErbB2 Precursor antibody

Tyrosine kinase-type cell surface receptor HER2 antibody

V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody

v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian) antibody

General Information

Product

ErbB 2 Antibody

Description

Affinity Purified Receptor tyrosine-protein kinase erB-2 isoform A Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Predicted: Rat

Immunogen

Synthetic peptide taken within amino acid region 1205-1255 receptor tyrosine-protein kinase erbB-2 protein.

Accession #

NCBI:  NP_004439.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-1.25 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P04626

Overview

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs).

Molecular Function

Activator, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein, Cytoplasm>perinuclear region, Nucleus. Isoform 2: Cytoplasm, Nucleus. Isoform 3: Cytoplasm, Nucleus.

Expression

Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

Structure

Homodimer. Heterodimer with EGFR, ERBB3 and ERBB4. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with PRKCABP and PLXNB1. Interacts (when phosphorylated on Tyr-1248) with MEMO1. Interacts with MUC1; the interaction is enhanced by heregulin (HRG).

Alternative Nomenclature

Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody

CD340 antibody

CerbB2 antibody

cneu antibody

Erb B2 antibody

erbb2 antibody

Erythroblastosis Virus antibody

HER 2 antibody

Herstatin antibody

Metastatic lymph node gene 19 protein antibody

MLN 19 antibody

NEU antibody

Neuro Glioblastoma Derived Oncogene Homolog antibody

NGL antibody

p185 ErbB2 antibody

Proto-oncogene Neu antibody

Receptor Protein Tyrosine Kinase ErbB2 Precursor antibody

Tyrosine kinase-type cell surface receptor HER2 antibody

V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody

v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian) antibody

General Information

Product

ErbB 4 Antibody

Description

Affinity Purified Receptor tyrosine-protein kinase erB-4 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1225-1275 on human Receptor tyrosine-protein kinase erbB-4.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-1.25 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q15303

Overview

Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactation. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis.

Molecular Function

ATP binding; epidermal growth factor receptor binding; protein homodimerization activity; receptor signaling protein tyrosine kinase activity; transcription regulatory region DNA binding; transmembrane receptor protein tyrosine kinase activity

Subcellular Location

Cell membrane; Single-pass type I membrane protein. ERBB4 intracellular domain: Nucleus; Mitochondrion

Expression

Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are expressed in cerebellum, but only the isoform JM-B is expressed in the heart.

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. 

Contains 1 protein kinase domain.

Alternative Nomenclature

4ICD antibody

ALS19 antibody

E4ICD antibody

EC 2.7.10.1 antibody

ERBB4 antibody

HER4 antibody

Mer4 antibody

MGC138404 antibody

Oncogene ERBB4 antibody

p180erbB4 antibody

Proto-oncogene-like protein c-ErbB-4 antibody

Receptor tyrosine protein kinase erbB 4 antibody

s80HER4 antibody

Tyrosine kinase type cell surface receptor HER4 antibodyv erb a avian erythroblastic leukemia viral oncogene homolog like 4 antibody

General Information

Product

MIG7 Antibody Affinity Purified

Description

Affinity Purified MIG7 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 170-220 on MIG7 protein.

Accession #

GenBank: ABF71424.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q1AHR6

Alternative Nomenclature

mig 7 antibody

General Information

Product

MUC1 Antibody

Description

Affinity Purified Mucin 1, transmembrane Antibody

Verified Applications

ELISA, IHC, IP

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on MUCIN1 protein.

Accession #

NCBI:  NM_182741.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Western Blot

1:500

Protein

Uniprot #

P15941

Overview

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.

Molecular Function

transcription cofactor activity

Subcellular Location

Cell membrane, Cytoplasm, Membrane, Nucleus, Secreted

Expression

Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform 7 is expressed in tumor cells only.

Alternative Nomenclature

ADMCKD antibody

Breast carcinoma-associated antigen DF3 antibody

CA 15 3 antibody

CD227 antigen antibody

DF3 antigen antibody

EMA antibody

Episialin antibody

Epithelial basement membrane antigen antibody

H23 antigen antibody

KL 6 antibody

MAM 6 antibody

MCD antibody

MCKD1 antibody

MUC 1 antibody

Peanut-reactive urinary mucin antibody

Polymorphic epithelial mucin antibody

Tumor associated epithelial membrane antigen antibody

General Information

Product

SEPT1 Antibody Affinity Purified

Description

Affinity Purified Septin 1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-350 on SEPT1 protein.

Accession #

Gen Bank: AAK61491.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.63-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8WYJ6

Overview

ilament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential).

Molecular Function

GTP binding

Subcellular Location

Cytoplasm, cytoskeleton, mictrotuble organizing center, centrosome; midbody. (Note: Remains at centrosomes and nearby microtubules throughout mitosis. Localizes to midbody during cytokinesis)

Expression

Expressed at high levels in lymphoid and hematopoietic tissues.

Alternative Nomenclature

DIFF6 antibody

Differentiation 6 (deoxyguanosine triphosphate triphosphohydrolase) antibody

LARP antibody

MGC20394 antibody

Peanut like protein 3 antibody

PNUTL3 antibody

SEP1 antibody

Serologically defined breast cancer antigen NY-BR-24 antibody

General Information

Product

Phospho-ErbB 2 Antibody

Description

Affinity Purified Receptor tyrosine-protein kinase erB-2 (HER2) Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Predicted: Human

Immunogen

Phoshporylated synthetic peptide taken within amino acid region 1100-1150 on human HER-2 protein

Accession #

GenBank:  AAA75493.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P04626

Overview

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs).

Molecular Function

Activator, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein, Cytoplasm>perinuclear region, Nucleus. Isoform 2: Cytoplasm, Nucleus. Isoform 3: Cytoplasm, Nucleus.

Expression

Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

Structure

Homodimer. Heterodimer with EGFR, ERBB3 and ERBB4. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with PRKCABP and PLXNB1. Interacts (when phosphorylated on Tyr-1248) with MEMO1. Interacts with MUC1; the interaction is enhanced by heregulin (HRG).

Alternative Nomenclature

Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody

c erb B2/neu protein antibody

CD340 antibody

CerbB2 antibody

Erb B2 antibody

Erythroblastosis Virus antibody

HER 2/neu antibody

Herstatin antibody

Metastatic lymph node gene 19 protein antibody

MLN19 antibody

NEU antibody

Neuro Glioblastoma Derived Oncogene Homolog antibody

NGL antibody

p185 ErbB2 antibody

Receptor Protein Tyrosine Kinase ErbB2 Precursor antibody

TKR1 antibody

Tyrosine kinase-type cell surface receptor HER2 antibody

v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian) antibody

verbb2 antibody