General Information

Product

DNA polymerase eta Antibody

Description

Affinity Purified Chain B, Yeast DNA polymerase Eta Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Saccharomyces cervisiae

Immunogen

Synthetic peptide taken within amino acid region 200-300 on Chain A, Yeast DNA polymerase Eta.

Accession #

NCBI:  1JIH_A

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.70-0.98 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q04049

Overview

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. Efficiently incorporates nucleotides opposite to other UV or oxidative DNA damages like O(6)-methylguanine, 7,8-dihydro-8-oxoguanine, 2,6-diamino-4-hydroxy-5-formamidopyrimidine of 2'-deoxyguanosine (FaPydG), or p-benzoquinone DNA adducts.

Molecular Function

Damaged DNA binding, Metal ion binding, DNA-directed DNA polymerase activity

Subcellular Location

Nucleus

Expression

Induced by UV radiation and heat shock.  The mRNA is stabilized during stationary phase.

Structure

Interacts with POL30. This interaction is essential for the polymerase eta function.

Alternative Nomenclature

DNA polymerase eta antibody

FLJ16395 antibody

FLJ21978 antibody

POLH antibody

polymerase DNA directed eta antibody

RAD30 antibody

RAD30 homolog A antibody

Xeroderma pigmentosum variant type protein antibody

XPV antibody

General Information

Product

Cytohesin 4 Antibody Affinity Purified

Description

Affinity Purified Cytohesin 4 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-362 on human Cytohesin 4 protein.

Accession #

GenBank: AAH41161.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.61 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9UIA0

Overview

Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF factors through replacement of GDP with GTP.

Molecular Function

ARF guanyl-nucleotide exchange factor activity, lipid binding

Subcellular Location

Cell membrane; Peripheral membrane protein

Expression

Expressed predominantly in peripheral blood leukocytes.

Structure

Contains 1 PH domain, Contains 1 SEC7 domain

Alternative Nomenclature

AI467541 antibody

CYT4 antibody

Cyth4 antibody

Cytohesin 4 antibody

DJ63G5.1 antibody

FLJ00017 antibody

PH antibody

PH, SEC7 and coiled coil domain containing protein 4 antibody

Pleckstrin homology, SEC7, AND coiled coil domains protein 4 antibody

Pscd4 antibody

RGD1564842 antibody

SEC7 and coiled-coil domain-containing protein 4 antibody

General Information

Product

TLR1 Antibody

Description

Affinity Purified Toll-like receptor 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Predicted: Human

Immunogen

Synthetic peptide taken within amino acid region 200-250 on mouse toll-like receptor 1 protein.

Accession #

NCBI:  NP_109607.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.67 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:20,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9EPQ1

Overview

Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides.

Molecular Function

Receptor, Transmembrane signaling receptor activity, triacyl lipopeptide binding

Subcellular Location

Cell membrane; Single-pass type I membrane protein, Cytoplasmic vesicle >  phagosome membrane; Single-pass type I membrane protein

Structure

Binds MYD88 (via TIR domains) (By similarity). Interacts (via extracellular domain) with TLR2. Ligand binding induces the formation of a heterodimer with TLR2 (By similarity). Interacts with CNPY3.

Alternative Nomenclature

CD281 antibody

KIAA0012 antibody

LPRS5 antibody

rsc786 antibody

Similar to Drosophila Toll protein antibody

TIL antibody

TIL. LPRS5 antibody

Tlr1 antibody

Toll (Drosophila) homolog antibody

Toll like receptor 1 antibody

Toll/Interleukin 1 receptor like antibody

General Information

Product

TLR1 Antibody

Description

Affinity Purified Toll-like receptor 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human,

Predicted: Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 370-420 on human Toll-like receptor 1.

Accession #

GenBank: AAI09094.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.67 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:20,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q15399

Overview

Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides.

Molecular Function

Receptor, Transmembrane signaling receptor activity, triacyl lipopeptide binding

Subcellular Location

Cell membrane; Single-pass type I membrane protein, Cytoplasmic vesicle >  phagosome membrane; Single-pass type I membrane protein

Structure

Binds MYD88 (via TIR domains) (By similarity). Interacts (via extracellular domain) with TLR2. Ligand binding induces the formation of a heterodimer with TLR2 (By similarity). Interacts with CNPY3.

Alternative Nomenclature

CD281 antibody

CD281 antigen antibody

KIAA0012 antibody

LPRS5 antibody

rsc786 antibody

Similar to Drosophila Toll protein antibody

TIL antibody

TIL. LPRS5 antibody

Tlr1 antibody

Toll (Drosophila) homolog antibody

Toll like receptor 1 antibody

Toll like receptor 1 Precursor antibody

Toll/Interleukin 1 receptor like antibody

Toll/interleukin-1 receptor-like protein antibody

General Information

Product

JMJD2D Antibody Affinity Purified

Description

Affinity Purified Jumonji domain-containing protein 2D Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 340-390 on human Jumonji domain containg 2D protein.

Accession #

GenBank: AAI19011.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q6B0I6

Overview

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Demethylates both di- and trimethylated H3 'Lys-9' residue, while it has no activity on monomethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.

Molecular Function

Chromatin regulator, Dioxygenase, Oxidoreductase

Subcellular Location

Nucleus

Structure

Belongs to the JHDM3 histone demethylase family.

Contains 1 JmjC domain.

Contains 1 JmjN domain.

Alternative Nomenclature

JHD3D antibody

JHDM3D antibody

JmjC domain-containing histone demethylation protein 3D antibody

Jumonji domain-containing protein 2D antibody

Kdm4d antibody

Lysine-specific demethylase 4D antibody

General Information

Product

Phospho-AKT1 Antibody

Description

Affinity Purified Phosphorylated Rac protein kinase-alpha Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Bird, Bovine, Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 430-480 on human Rac protein kinase-alpha protein.

Accession #

GenBank: AAA36539.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P31749

Overview

One of 3 serine/threonine-protein kinases (AKT1-AKT3) regulates many processes including metabolism, proliferation, cell survival, growth and angiogenesis.

Molecular Function

Developmental protein; Kinase; Serine/threonine-protein kinase; Transferase

Subcellular Location

Cytoplasm; Nucleus; Cell membrane

Expression

Expressed in prostate cancer and all human cell types.

Structure

Interacts with and phosphorylated by PDPK1.  Interacts with several proteins invovled in phosphorylation and ubiquitination

Alternative Nomenclature

AKT 1 antibody

C AKT antibody

MGC9965 antibody

MGC99656 antibody

Oncogene AKT1 antibody

PKB antibody

PKB-ALPHA antibody

PRKBA antibody

Protein Kinase B Alpha antibody

Protein kinase B antibody

Proto-oncogene c-Akt antibody

RAC Alpha antibody

RAC antibody

RAC PK Alpha antibody

RAC Serine/Threonine Protein Kinase antibody

RAC-PK-alpha antibody

vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody

General Information

Product

SHQ1 Antibody Affinity Purified

Description

Affinity Purified Shq1p Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

S. cerevisiae

Immunogen

Synthetic peptide taken within amino acid region 40-90 on S. cerevisiae protein.

Accession #

GenBank: AJR51992.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P40486

Overview

Involved in the early biogenesis steps of box H/ACA snoRNP assembly.

Molecular Function

Unfolded protein binding; Box H/ACA snoRNP assembly

Subcellular Location

Nucleus

Structure

Interacts with CBF5, NAF1 and NHP2. The interaction with NAF1 and SHQ1 does not occur when NAF1 is associated with the chromatin. Contains 1 CS domain; Belongs to the SHQ1 family

Alternative Nomenclature

FLJ10539 antibody

Protein SHQ1 homolog antibody

shq1 antibody

SHQ1 homolog (S. cerevisiae) antibody

SHQ1 homolog antibody

SHQ1, H/ACA ribonucleoprotein assembly factor antibody

Shq1p antibody

General Information

Product

Smad1 Antibody Affinity Purified

Description

Affinity Purified Mothers against dpp Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 405-455 on D. melanogaster Mothers against dpp protein.

Accession #

NCBI:  NP_477017.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.0 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P42003

Overview

Required for the function of decapentaplegic. May play an important role in mediating Dpp signaling. Involved in the BMP signaling pathway.

Molecular Function

DNA binding; RNA polymerase II activating transcription factor; Transcription regulatory region sequence-specific activity; Transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity

Subcellular Location

Cytoplasm; Nucleus

Expression

Is detected in all developmental stages, though it appears most abundant in pupae, adult stages and early embryos. Its abundance decreases throughout embryonic and larval development and then returns to high levels in pupae and adult females.

Structure

Homotrimer

Alternative Nomenclature

BSP 1 antibody

Dwarfin A antibody

DwfA antibody

JV 41 antibody

MAD homolog 1 antibody

MAD mothers against decapentaplegic homolog 1 antibody

Mad related protein 1 antibody

MADR1 antibody

Mothers against DPP homolog 1 antibody

Sma and Mad related protein 1 antibody

SMAD 1 antibody

Spinal muscular atrophy distal with upper limb predominance antibody

TGF beta signaling protein 1 antibody

Transforming growth factor beta signaling protein 1 antibody

General Information

Product

NM23A Antibody Affinity Purified

Description

Affinity Purified Nucleoside diphosphate kinase A Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 1-100 on human NDKA protein.

Accession #

GenBank: AAO85436.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P15531

Overview

Major role in the synthesis of nucleoside triphosphates other than ATP.

Molecular Function

Kinase, Transferase

Subcellular Location

Cytoplasm, Nucleus

Expression

heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen,thymus and lung carcinoma cell lines

Structure

Hexamer of two different chains: A and B

Alternative Nomenclature

AWD antibody

GAAD antibody

Granzyme A activated DNase antibody

Metastasis inhibition factor nm23 antibody

NB antibody

NDK A antibody

NM23 antibody

NME 1 antibody

Non metastatic cells 1 protein antibody

Nonmetastatic protein 23 antibody

Nucleoside diphosphate kinase A antibody

Tumor metastatic process associated protein antibody

General Information

Product

NME2 + NM23A Antibody

Description

Affinity Purified Nucleoside diphosphate kinase A and Nucleoside diphosphate kinase B Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 100-150 on NM23A protein selective for both NDKA and NDKB.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P15531

Overview

Major role in the synthesis of nucleoside triphosphates other than ATP.

Molecular Function

Kinase, Transferase

Subcellular Location

Cytoplasm, Nucleus

Expression

heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen,thymus and lung carcinoma cell lines

Structure

Hexamer of two different chains: A and B

Alternative Nomenclature

C myc purine binding transcription factor PUF antibody

Histidine protein kinase NDKB antibody

MGC111212 antibody

MGC2212 antibody

NDK B antibody

NM23B antibody

nme2 antibody

Nucleoside diphosphate kinase B antibody

PUF antibody