General Information

Product

MAVS Antibody

Description

Affinity Purified Mitochondrial Anti Viral-Signaling Protein Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 500-540 on on human mitochondrial antiviral-signaling protein isoform 1.

Accession #

NCBI:  NP_065797

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q7Z434

Overview

Required for innate immune defense against viruses. Acts downstream of DDX58/RIG-I and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). Peroxisomal and mitochondrial MAVS act sequentially to create an antiviral cellular state. Upon viral infection, peroxisomal MAVS induces the rapid interferon-independent expression of defense factors that provide short-term protection, whereas mitochondrial MAVS activates an interferon-dependent signaling pathway with delayed kinetics, which amplifies and stabilizes the antiviral response. May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis.

Molecular Function

Antiviral defense, Host-virus interaction, Immunity, Innate immunity

Subcellular Location

Mitochondrion outer membrane, Mitochondrion, Peroxisome

Expression

Present in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes.

Structure

Both CARD and transmembrane domains are essential for antiviral function. The CARD domain is responsible for interaction with DDX58/RIG-I and IFIH1/MDA5.

The transmembrane domain and residues 300-444 are essential for its interaction with DHX58/LGP2.

Alternative Nomenclature

CARD adapter inducing interferon beta antibody

IFN B promoter stimulator 1 antibody

Interferon beta promoter stimulator protein 1 antibody

Ips 1 antibody

MAVS antibody

Mitochondrial anti viral signaling protein antibody

Putative NF kappa B activating protein 031N antibody

Virus induced signaling adapter antibodyVISA antibody

General Information

Product

Phospho-STAT1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Predicted:  Rat

Immunogen

Synthetic peptide taken within amino acid region 700-750 on human STAT1 protein. Phosphorylated Serine 727 residue.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P42224

Overview

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.

Molecular Function

Activator

Subcellular Location

Cytoplasm; Nucleus

Structure

Isoform alpha homodimerizes upon IFN-gamma induced phosphorylation. Heterodimer with STAT2 upon IFN-alpha/beta induced phosphorylation. The heterodimer STAT1:STAT2 forms the interferon-stimulated gene factor 3 complex (ISGF3) with IRF9. Interacts (phosphorylated at Ser 727) with PIAS1 (dimethylated on arginine); the interaction results in release of STAT1 from its target gene. Interacts with IFNAR1; the interaction requires the phosphorylation of IFNAR1 at 'Tyr-466'. Interacts with IFNAR2, NMI, PTK2/FAK1 and SRC. Interacts with ERBB4 (phosphorylated). Interacts with Sendai virus C', C, Y1 and Y2 proteins, Nipah virus P, V and W proteins, and rabies virus phosphoprotein preventing activation of ISRE and GAS promoter. Interacts with HCV core protein; the interaction results in STAT1 degradation.

Alternative Nomenclature

Signal transducer and activator of transcription 1 91kD 
CANDF7 
IMD31A 
IMD31B 
IMD31C 
ISGF 3 
Signal transducer and activator of transcription 1-alpha/beta 
STAT91 
Transcription factor ISGF-3 components p91/p84 

General Information

Product

Phospho-STAT4 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 690-740 on human Signal transudcer and activator of transcription 4 protein. Phosphorylated serine 721 residue.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q14765

Overview

Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.

Molecular Function

Transcription, Transcription regulation

Subcellular Location

Cytoplasm; Nucleus

Structure

Forms a homodimer or a heterodimer with a related family member. Interacts with ARL2BP. The SH2 domain interacts, in vitro, with IL12RB2 via a short cytoplasmic domain.

Alternative Nomenclature

Signal transducer and activator of transcription 4 antibody

SLEB11 antibody

STAT4 antibody

General Information

Product

STAT6 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse

Predicted: Rat

Immunogen

Synthetic peptide taken within amino acid region 655-705 on human Signal transducer and activator of transcription 6 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P42226

Overview

Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

Molecular Function

Activator; DNA-binding

Subcellular Location

Cytoplasm; Nucleus

Structure

Forms a homodimer or a heterodimer with a related family member. Interacts with NCOA1 via its C-terminal LXXLL motif.

Alternative Nomenclature

IL 4 STAT antibody

Interleukin 4 Induced antibody
Interleukin 4 Induced Transcription Factor IL4 STAT antibody
Signal transducer and activator of transcription 6 antibody
Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody

STAT interleukin4 induced antibody

STAT6B antibody
STAT6C antibody
Transcription factor IL 4 STAT antibody

General Information

Product

SQSTM1 / p62 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-440 on human Sequestosome-1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q13501

Overview

Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD. May be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels.

Molecular Function

identical protein binding, protein kinase binding, protein kinase C binding, protein serine/threonine kinase activity, receptor tyrosine kinase binding, SH2 domain binding, ubiquitin binding, ubiquitin protein ligase binding, zinc ion binding

Subcellular Location

Cytoplasm

Late endosome

Lysosome

Cytoplasmic vesicle › autophagosome

Nucleus

Endoplasmic reticulum

Cytoplasm › P-body

Expression

Ubiquitously expressed.

Structure

Homooligomer or heterooligomer; may form homotypic arrays. Dimerization interferes with ubiquitin binding.

Alternative Nomenclature

A170 

EBI 3 associated protein of 60 kDa 

EBIAP 

FTDALS3 

ORCA 

OSF-6 

Osi 

OSIL 

Oxidative stress induced like 

p60 

p62 

p62B 

Paget disease of bone 3 

PDB3 

Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa 

PKC-zeta-interacting protein 

Protein kinase C-zeta-interacting protein 

Sequestosome 1 

SQSTM 1 

STAP 

STONE14 

Ubiquitin-binding protein p62 

ZIP 

ZIP3 

General Information

Product

GPS2 Antibody

Description

Affinity Purified G protein pathway suppressor 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-300 on human G protein pathway suppressor 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q13227

Molecular Function

cyclin binding

GTPase inhibitor activity

transcription coactivator activity

transcription corepressor activity

Subcellular Location

Nucleus, Cytosol

Expression

Widely expressed.

Structure

Coiled coil

Alternative Nomenclature

AMF 1 antibody

G protein pathway suppressor 2 antibody

GPS 2 antibody

General Information

Product

Dnmt1 Antibody

Description

Affinity Purified DNA (cytosine-5)-methyltransferase 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 1550-1600 on human Dnmt1 protein.

Physical Properties

Quantity

100 µg

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Conjugate

Unconjugated

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:100

Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50-1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P26358

Molecular Function

DNA (cytosine-5-)-methyltransferase activity

DNA binding

DNA-methyltransferase activity

methyl-CpG binding

promoter-specific chromatin binding

RNA binding

zinc ion binding

Subcellular Location

Nucleus

Alternative Nomenclature

ADCADN antibody

AIM antibody

CXXC finger protein 9 antibody

CXXC-type zinc finger protein 9 antibody

CXXC9 antibody

DNA (cytosine 5 ) methyltransferase 1 antibody

DNA methyltransferase 1 antibody

DNA methyltransferase HsaI antibody

DNA methyltransferase M.HsaI. antibody

DNA MTase HsaI antibody

DNMT 1 antibody

DNMT1_HUMAN antibody

Dnmt1o antibody

FLJ16293 antibody

HSN1E antibody

M.HsaI antibody

MCMT antibody

Met1 antibody

MGC104992 antibody

mMmul antibody

MommeD2 antibody