General Information
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Product
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Phospho Src Antibody BIOTIN-Conjugated
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Description
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BIOTIN-Conjugated Phosphorylated Proto-oncogene tyrosine-protein kinase Src Antibody C-epitope
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Verified Applications
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ELISA, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Mouse, Rat
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Immunogen
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C-epitope phosphorylated synthetic peptide taken within amino acid region 400-500 on human Src protein. Phosphorylated Tyr-416 residue.
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Accession #
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NCBI: NP_005408.1
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.65-1.25 µg/µl in antibody stabilization buffer
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Form
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BIOTIN-Conjugated
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Clonality
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Polyclonal
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Determinant
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C-epitope
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:10,000
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ELISA
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1:10,000
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Immunoprecipitation |
1:200 |
Western Blot
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1:500
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Protein
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Uniprot #
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P12931
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Overview
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Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis migration, and transformation.
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Molecular Function
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ATP binding, ephrin receptor binding, heme binding, ion channel binding, enzyme binding, growth factor receptor binding, integrin binding, kinase activity
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Subcellular Location
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Cell membrane, mitochondrion inner membrane, nucleus, cytoplasm (Note: Localizes to focal adhesion sites following integrin engagement. Localization to focal adhesion sites requires myristoylation and SH3 domain.)
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Expression
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Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.
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Alternative Nomenclature
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ASV antibody
Avian sarcoma virus antibody
Neuronal CSRC tyrosine specific protein kinase antibody
p60 Src antibody
SRC1 antibody
Tyrosine kinase pp60c src antibody
v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody
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