General Information

Product

CCR5 Antibody

Description

Affinity Purified Chemokine receptor type 5 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human CCR5 protein.

Accession #

NCBI: AAB65720.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P51681

Overview

Receptor for several inflammatory C-C chemokines.  May play a role in control of granulocytic lineage proliferation or differentiation.

Molecular Function

actin binding; C-C chemokin binding; GPCR; Receptor; Transducer; Host-virus interaction

Subcellular Location

Cell membrane; Mulipass membrane protein.

Expression

Spleen, Thymus, myeloid cell line THP-1, promyeloblastic cell line KG-1a and on CD4+ and CD8+ T-cells.  Lesser expressed in peripheral blood leukocytes, small intestine and ovary and lung.

Structure

Interacts with PRAF2, HIV-1 surface protein gp120. Glycosylation on Ser-6 is required for binding with CCL4.

Alternative Nomenclature

AM4 7 antibody

CCCKR 5 antibody

CD 195 antibody

CHEMR13 antibody

HIV 1 Fusion Coreceptor antibody

IDDM22 antibody

MIP-1 alpha receptor antibody

General Information

Product

CCR2 Antibody

Description

Affinity Purified C-C chemokine receptor type 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 20-70 on human CCR2 protein.

Accession #

NCBI: NP_955783

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.61-0.71 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P41597

Overview

Receptor for CCL2, CCL7 and CCL13 chemokines.  Transduces a signal by increasing intracellular calcium ion levels.  Alternative coreceptor with CD4 for HIV-1 infection.

Molecular Function

Host-virus interaction; GPCR; Transducer

Subcellular Location

Cell membrane; Mulipass membrane protein.

Expression

Induced; Up-regulated by CREB3

Structure

Binds to HIV-1 Tat.  Interacts with ARRB1

Alternative Nomenclature

CC CKR 2 antibody

CD192 antibody

CKR2A antibody

CKR2B antibody

CMKBR2 antibody

MCP1 RECEPTOR antibody

Monocyte chemoattractant protein 1 receptor antibody

General Information

Product

CXCR4 Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 15-65 on CXCR4 protein.

Accession #

GenBank:  CAA12166

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

P61073

Overview

Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes.

Molecular Function

Host-virus Interaction

Subcellular Location

Cell membrane; Multi-pass membrane protein. Cell junction. Early endosome. Late endosome. Lysosome

Expression

Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.

Structure

Monomer. Can form dimers.

Alternative Nomenclature

C-X-C chemokine receptor type 4 antibody

CD184 antibody

Chemokine CXC Motif Receptor 4 antibody

CXCR4 antibody

D2S201E antibody

FB22 antibody

Fusin antibody

HM89 antibody

HSY3RR antibody

LAP 3 antibody

LCR1 antibody

LESTR antibody

Leukocyte derived seven transmembrane domain receptor antibody

Lipopolysaccharide associated protein 3 antibody

Neuropeptide Y receptor Y3 antibody

NPY3R antibody

NPYR antibody

NPYRL antibody

Probable G protein coupled receptor lcr1 homolog antibody

SDF 1 receptor antibody

Stromal cell derived factor 1 receptor antibody

WHIM antibody

General Information

Product

CXCR6 Antibody

Description

Affinity Purified C-X-C chemokine receptor type 6 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Gorilla, Monkey

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human CXCR6 protein.

Accession #

NCBI:  NP_006555

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

O00574

Overview

Receptor for the C-X-C chemokine CXCL16. Used as a coreceptor by SIVs and by strains of HIV-2 and m-tropic HIV-1.

Molecular Function

G-protein coupled receptor, Receptor, Transducer

Subcellular Location

Cell membrane; Multi-pass membrane protein

Expression

Expressed in lymphoid tissues and activated T cells.

Structure

G-protein coupled receptor 1 family; Transmembrane helix

Alternative Nomenclature

C X C chemokine receptor type 6 antibody

CD186 antigen antibody

Chemokine (C X C motif) receptor 6 antibody

CXCR-6 antibody

G protein coupled receptor bonzo antibody

G protein coupled receptor STRL33 antibody

G protein coupled receptor TYMSTR antibody

General Information

Product

SEPT6 Antibody Affinity Purified

Description

Affinity Purified Septin 6 Mrna Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 370-430 on SEPT6 protein.

Accession #

Gen Bank: AF397023.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.54-0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q548C9

Molecular Function

GTP binding

Subcellular Location

Septin complex

Structure

Belongs to Septin Family, Contains septin-type G (guanine nucleotide-binding) domain.

Alternative Nomenclature

KIAA0128 antibody

Nkrf antibody

RCG53214, isoform CRA_d antibody

RP5-876A24.2 antibody

SEP2 antibody

SEPT2 antibody

SEPT6/MLL FUSION GENE antibody

Septin 2 antibody

Septin 6 antibody

General Information

Product

SIRT1 Antibody

Description

Affinity Purified NAD-dependent protein deacetylase sirtuin-1 isoform 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid 25-75 on rat Sirtuin 1 protein.

Accession #

NCBI:  NP_062786.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.69-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q923E4

Overview

NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in coordination of several separated cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis and autphagy.

Subcellular Location

Nucleus, PML body, cytoplasm. (Nucleus: Recruited to the nuclear bodies via its interaction with the PML. Colocalized with APEX1 in nucleus. May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. Shuttles between nucleus and cytoplasm).

Expression

Widely expressed. Weakly expressed in liver and skeletal muscle.

Alternative Nomenclature

75SirT1 antibody

HST2, S. cerevisiae, homolog of antibody

NAD dependent deacetylase sirtuin 1 antibody

Regulatory protein SIR2 homolog 1 antibody

SIR2 like protein 1 antibody

SIR2, S.cerevisiae, homolog-like 1 antibody

SIR2ALPHA antibody

SIR2L1 antibody

SirtT1 75 kDa fragment antibody

Sirtuin type 1 antibody

General Information

Product

STING / TMEM173 Antibody

Description

Affinity Purified Stimulator of interferon genes protein isoform 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acid region 332-342. The selected peptide was post-synthetically modified to achieve highest antigenicity before coupling to a carrier protein using hetero bifunctional cross linker.

Sequence:

SFSLLSSQEVLRHLLRQEEKEEVT

Accession #

NCBI:  NP_938023

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

86WV6

Overview

Facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm.

Molecular Function

cyclic-di-GMP binding, cyclic-GMP-AMP binding, identical protein binding

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion outer membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cytoplasm › perinuclear region. Cytoplasm

Expression

Ubiquitously expressed. Expressed in skin endothelial cells, alveolar type 2 pneumocytes, bronchial epithelium and alveolar macrophages.

Structure

Associates with the MHC-II complex (By similarity). Homodimer; 'Lys-63'-linked ubiquitination at Lys-150 is required for homodimerization. Interacts with DDX58/RIG-I, MAVS and SSR2. Interacts with RNF5 and TRIM56. Interacts with TBK1; when homodimer, leading to subsequent production of IFN-beta. Interacts with IFIT1 and IFIT2.

Alternative Nomenclature

endoplasmic reticulum IFN stimulator antibody

ERIS antibody

FLJ38577 antibody

Mediator of IRF3 activation antibody

MITA antibody

Mitochondrial mediator of IRF3 activation antibody

MPYS antibody

N terminal methionine proline tyrosine serine plasma membrane tetraspanner antibody

NET23 antibody

Stimulator of interferon genes antibody

STING antibody

Tmem173 antibody

Transmembrane protein 173 antibody

General Information

Product

TLR3 Antibody

Description

Affinity Purified Toll-like receptor 3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 650-700 on human TLR3 protein.

Accession #

GenBank: AAH96333.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.77 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:20,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O15455

Overview

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection.

Molecular Function

Receptor, double-stranded RNA binding, transmembrane signaling receptor activity

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type I membrane protein, Endosome membrane

Expression

Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.

Structure

Monomer and homodimer; dimerization is triggered by ligand-binding, the signaling unit is composed of one ds-RNA of around 40 bp and two TLR3 molecules, and lateral clustering of signaling units along the length of the ds-RNA ligand is required for TLR3 signal transduction. Interacts (via transmembrane domain) with UNC93B1; the interaction is required for transport from the ER to the endosomes.

Alternative Nomenclature

CD283 antibody

CD283 antigen antibody

IIAE2 antibody

TLR 3 antibody

Toll Like Receptor 3 antibody

General Information

Product

TLR4 Antibody Affinity Purified

Description

Affinity Purified Toll-like receptor 4 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 970-1000 on human TLR4 protein.

Accession #

GenBank: AAF05316.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.66-0.8 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500

Protein

Uniprot #

O00206

Overview

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni2+.

Molecular Function

Receptor, lipopolysaccharide binding, transmembrane signaling receptor activity

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.

Structure

Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Binding to bacterial LPS leads to homodimerization. Interacts with LY96 via the extracellular domain. Interacts with MYD88 and TIRAP via the

Alternative Nomenclature

ARMD10 antibody

CD284 antibody

Homolog of Drosophila toll antibody

hToll antibody

TLR 4 antibody

Toll (Drosophila) homolog antibody

TOLL antibody

Toll Endotoxin Hyporesponsiveness antibody

Toll like receptor 4 antibody

General Information

Product

VPRBP Antibody

Description

Affinity Purified VPR Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human Immunodeficiency Virus 1

Immunogen

Synthetic peptide taken within amino acid region 1-27 on human VPR protein.

Accession #

GenBank: AGV39791.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.52 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

T2D0A6

Overview

During virus entry, involved in the transport of the viral pre-integration (PIC) complex to the nucleus (PubMed:12417576). This function is crucial for viral infection of non-dividing macrophages (PubMed:12417576). May act directly at the nuclear pore complex, by binding nucleoporins phenylalanine-glycine (FG)-repeat regions. During virus replication, may deplete host UNG protein, and incude G2-M cell cycle arrest(PubMed:7666531, PubMed:7474100). Acts by targeting specific host proteins for degradation by the 26S proteasome, through association with the cellular CUL4A-DDB1 E3 ligase complex by direct interaction with host VPRPB/DCAF-1. Cell cycle arrest reportedly occurs within hours of infection and is not blocked by antiviral agents, suggesting that it is initiated by the Vpr carried into the virion. Additionally, Vpr induces apoptosis in a cell cycle dependent manner suggesting that these two effects are mechanistically linked. Detected in the serum and cerebrospinal fluid of AIDS patient, Vpr may also induce cell death to bystander cells (PubMed:11000244).

Molecular Function

Viral Life Cycle

Subcellular Location

Virion; Host nucleus; Host Extracellular Space

Structure

Homooligomer, may form homodimer (PubMed:7798208). Interacts with p6-gag region of the Pr55 Gag precursor protein through a (Leu-X-X)4 motif near the C terminus of the P6gag protein (PubMed:7474102). Interacts with host UNG (PubMed:9151883). May interact with host RAD23A/HHR23A (PubMed:9371639). Interacts with host VPRBP/DCAF1, leading to hijack the CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, mediate ubiquitination of host proteins such as TERT and ZGPAT and arrest the cell cycle in G2 phase (PubMed:17314515, PubMed:17630831, PubMed:17314515).

Alternative Nomenclature

DCAF1 antibody

DDB1 and CUL4 associated factor 1 antibody

HIV-1 Vpr-binding protein antibody

KIAA0800 antibody

protein VPRBP antibody

RIP antibody

Vpr (HIV-1) binding protein antibody

VPR BP antibody

Vpr interacting protein antibody

Vpr-binding protein antibody