General Information
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Product
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Dopamine Receptor D3 Antibody FITC
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Description
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FITC-Conjugated D(3) dopamine receptor Antibody
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Verified Applications
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Dot Blot, ELISA, ICC/IF, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Mouse, Rat
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Immunogen
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Synthetic peptide corresponding to unique amino acid sequence on Dopamine Receptor D3 protein, peptide was covalently modified.
Sequence: CQACHVSPELYRATTWGY
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.67 µg/µl in antibody stabilization buffer
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Form
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FITC-Conjugated
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Clonality
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Polyclonal
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Storage
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-20°C for long term storage
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Application Protocol
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DB
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1:50,000
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ELISA
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1:50,000
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Immunofluorescence |
Use at a concentration of 5 µg/µl |
Immunoprecipitation
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Use at an assay dependent dilution. 2 µl of this antibody will immunoprecipitate 65-80% dopamine recombinant D3 receptor protein from COS1 cells.
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Western Blot
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1:1,000-1:1,5000. Detects a band of approximately 50 kDa (predicted molecular weight 50 kDa).
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Protein
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Uniprot #
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P19020
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Overview
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Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation (By similarity).
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Molecular Function
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G-protein coupled receptor, Receptor, Transducer
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Subcellular Location
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Cell membrane, Multi-pass membrane protein
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Expression
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Mainly in limbic areas of brain.
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Structure
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Interacts with GRK4 and PALM (By similarity). Interacts with CLIC6.
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Alternative Nomenclature
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3dopamine receptor antibody
D antibody
D(3) dopamine receptor antibody
DRD3 antibody
ETM1 antibody
FET1 antibody
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