General Information
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Product
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DNA polymerase eta Antibody
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Description
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Affinity Purified Chain B, Yeast DNA polymerase Eta Antibody
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Verified Applications
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ELISA, WB
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Host
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Rabbit
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Species Cross Reactivity
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Saccharomyces cervisiae
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Immunogen
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Synthetic peptide taken within amino acid region 200-300 on Chain A, Yeast DNA polymerase Eta.
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Accession #
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NCBI: 1JIH_A
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.70-0.98 µg/µl in antibody stabilization buffer
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Form
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Affinity Purified
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:10,000
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ELISA
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1:10,000
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Western Blot
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1:500
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Protein
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Uniprot #
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Q04049
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Overview
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DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. Efficiently incorporates nucleotides opposite to other UV or oxidative DNA damages like O(6)-methylguanine, 7,8-dihydro-8-oxoguanine, 2,6-diamino-4-hydroxy-5-formamidopyrimidine of 2'-deoxyguanosine (FaPydG), or p-benzoquinone DNA adducts.
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Molecular Function
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Damaged DNA binding, Metal ion binding, DNA-directed DNA polymerase activity
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Subcellular Location
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Nucleus
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Expression
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Induced by UV radiation and heat shock. The mRNA is stabilized during stationary phase.
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Structure
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Interacts with POL30. This interaction is essential for the polymerase eta function.
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Alternative Nomenclature
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DNA polymerase eta antibody
FLJ16395 antibody
FLJ21978 antibody
POLH antibody
polymerase DNA directed eta antibody
RAD30 antibody
RAD30 homolog A antibody
Xeroderma pigmentosum variant type protein antibody
XPV antibody
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