General Information

Product

DNA PKcs Antibody

Description

Affinity Purified DNA-dependent protein kinase catalytic subunit Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 4000-4128 of human DNPK1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P78527

Overview

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Nucleus

Expression

Found in axons of the central and peripheral nervous system;differentiated cell types of the intestine

Structure

Belongs to the immunoglobulin superfamily. DCC family;Contains 6 fibronectin type-III domains;Contains 4 Ig-like C2-type (immunoglobulin-like) domains

Alternative Nomenclature

DNA dependent protein kinase catalytic subunit antibody
DNA PK catalytic subunit antibody
DNA-PKcs antibody
DNAPK antibody
DNPK1 antibody
Hyper radiosensitivity of murine scid mutation, complementing 1 antibody
HYRC antibody
HYRC1 antibody
p350 antibody
p460 antibody
PKRDC antibody
Protein Kinase DNA Activated Catalytic Polypeptide antibody
XRCC7 antibody

General Information

Product

Phospho Lyn Antibody Affinity Purified

Description

Affinity Purified Tyrosine-protein kinase Lyn isoform A Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Immunogen

Phosphorylated synthetic peptide taken within amino acid region 425-525 on human Lyn protein. Phospho Y 491.

Accession #

NCBI:  NP_002341

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P07948

Overview

Non-receptor tyrosine-protein kinase that plays role in regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents

Molecular Function

Kinase, Transferase, Tyrosine-protein kinase

Subcellular Location

Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Nucleus

Expression

monocytes,placenta,fetal brain, lung, liver,kidney and epidermoid, hematopoietic, and neuronal cells,also primary neuroblastoma tumors

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily

Alternative Nomenclature

AA407514 antibody

EC 2.7.10.2 antibody

FLJ26625 antibody

Hck 2 antibody

JTK 8 antibody

Lck/Yes related novel protein tyrosine kinase antibody

LYN antibody

LYN proto oncogene, Src family tyrosine kinase antibody

ONCOGENE LYN antibody

p53Lyn antibody

p56Lyn antibody

Tyrosine protein kinase LYN antibody

V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody

General Information

Product

PRP19 Antibody Affinity Purified

Description

Affinity Purified pre-mRNA-processing factor 19 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 200-300 on rat PRP19F protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:2,000

ELISA

1:2,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q9JMJ4

Overview

Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3.

Molecular Function

Ligase, DNA binding, ubiquitin-ubiquitin ligase activity

Structure

Homotetramer. Component of the Prp19 complex/PRP19C/Nineteen complex/NTC and related complexes described as PRP19-CDC5L splicing complex and PSO4 complex. A homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2 constitute the core of those complexes. The interac

Alternative Nomenclature

hPso4 antibody

NMP200 antibody

Nuclear matrix protein 200 antibody

pre mRNA processing factor 19 antibody

PRP19 antibody

PRP19/PSO4 homolog antibody

PRPF19 antibody

PSO4 antibody

psoralen 4 antibody

Senescence evasion factor antibody

SNEV antibody

UBOX4 antibody

General Information

Product

DNA polymerase eta Antibody

Description

Affinity Purified Chain B, Yeast DNA polymerase Eta Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Saccharomyces cervisiae

Immunogen

Synthetic peptide taken within amino acid region 200-300 on Chain A, Yeast DNA polymerase Eta.

Accession #

NCBI:  1JIH_A

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.70-0.98 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q04049

Overview

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. Efficiently incorporates nucleotides opposite to other UV or oxidative DNA damages like O(6)-methylguanine, 7,8-dihydro-8-oxoguanine, 2,6-diamino-4-hydroxy-5-formamidopyrimidine of 2'-deoxyguanosine (FaPydG), or p-benzoquinone DNA adducts.

Molecular Function

Damaged DNA binding, Metal ion binding, DNA-directed DNA polymerase activity

Subcellular Location

Nucleus

Expression

Induced by UV radiation and heat shock.  The mRNA is stabilized during stationary phase.

Structure

Interacts with POL30. This interaction is essential for the polymerase eta function.

Alternative Nomenclature

DNA polymerase eta antibody

FLJ16395 antibody

FLJ21978 antibody

POLH antibody

polymerase DNA directed eta antibody

RAD30 antibody

RAD30 homolog A antibody

Xeroderma pigmentosum variant type protein antibody

XPV antibody

General Information

Product

Histone H3 Antibody

Description

Affinity Purified Histone H3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Pedicted: Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human HH3 protein.

Accession #

GeneBank: CAA58540.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P68431

Overview

Core component of nucleosome.Play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Molecular Function

DNA-binding

Subcellular Location

Nucleus; Chromosome

Expression

During Sphase, then expression decreases as cell division slows down during the process of differentiation

Structure

Belongs to histone H3 family

Alternative Nomenclature

H3.3B antibody

H3F3A antibody

H3 histone antibody

H3.3A antibody

H3F3 antibody

H3f3b antibody

HIST3H3 antibody

Histone H3 3 pseudogene antibody

PP781 antibody

General Information

Product

Histone H3 Antibody

Description

Affinity Purified Histone H3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, S. cerevisiae

Immunogen

Synthetic peptide taken within amino acid 1-50 on Sacchoromyces cerevisiae protein HH3 protein.

Accession #

Gen Bank: EDZ69636.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

B5VR08

Overview

Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones. Can bind to DNA (in vitro).

Molecular Function

Chromatin regulator, Methyltransferase, Transferase

Subcellular Location

Nucleus

Structure

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.

Contains 1 DOT1 domain.

Alternative Nomenclature

H3 histone family, member J antibody

H3 histone antibody

H3 histone family, member A antibody

H3 histone family, member B antibody

H3 histone family, member C antibody

H3 histone family, member D antibody

H3 histone family, member F antibody

H3 histone family, member H antibody

H3 histone family, member I antibody

H3 histone family, member K antibody

H3 histone family, member L antibody

H3 histone, family 3A antibody

H3.3A antibody

H3F1K antibody

H3F3 antibody

Histone cluster 1, H3a antibody

Histone cluster 1, H3b antibody

Histone cluster 1, H3c antibody

Histone cluster 1, H3d antibody

Histone cluster 1, H3e antibody

Histone cluster 1, H3f antibody

Histone cluster 1, H3g antibody

Histone cluster 1, H3i antibody

Histone cluster 1, H3j antibody

General Information

Product

GPCR GPR87 Antibody

Description

Affinity Purified G-protein coupled receptor 87 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Panda, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-60 on human GPCR87 protein.

Accession #

NCBI: NP_076404

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9BY21

Overview

Receptor for lysophosphatidic acid.  Necessary for p53/TP53-dependent survival in response to DNA damage.

Molecular Function

G-protein coupled receptor, Receptor, Transducer

Subcellular Location

Cell membrane; Multi-pass membrane protein

Expression

Placenta and prostate. Weaker expression in thymus. Overexpressed in squamous cell carcinoma of the lung.

Structure

Belongs to G-protein coupled receptor 1 family

Alternative Nomenclature

FKSG 78 antibody

G-protein coupled receptor 87 antibody

G-protein coupled receptor 95 antibody

GPR 95 antibody

hGPCR87 antibody

KPG_002 antibody

MGC131898 antibody

Orphan GPCR 87 antibody

Probable G protein coupled receptor 87 antibody

General Information

Product

Lyn Antibody

Description

Affinity Purified Tyrosine-protein kinase Lyn Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Immunogen

Synthetic peptide taken within amino acid region 425-525 on human Lyn protein.

Accession #

NCBI:  NP_002341

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P07948

Overview

Non-receptor tyrosine-protein kinase that plays role in regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents

Molecular Function

Kinase, Transferase, Tyrosine-protein kinase

Subcellular Location

Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Nucleus

Expression

monocytes,placenta,fetal brain, lung, liver,kidney and epidermoid, hematopoietic, and neuronal cells,also primary neuroblastoma tumors

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily

Alternative Nomenclature

AA407514 antibody

EC 2.7.10.2 antibody

FLJ26625 antibody

Hck 2 antibody

JTK 8 antibody

Lck/Yes related novel protein tyrosine kinase antibody

LYN antibody

ONCOGENE LYN antibody

p53Lyn antibody

p56Lyn antibody

Tyrosine protein kinase LYN antibody

V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody

General Information

Product

PELO Antibody

Description

Affinity Purified PELOTA Antibody

Verified Applications

DB, ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 330-380 on human PELOTA protein.

Accession #

GenBank: AAG22575.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q9BRX2

Overview

Is necessary in cell division for normal chromosome segregation and genomic stability.  It may recognize stalled ribosomes and trigger endonucleolytic cleavage of the mRNA, and may have ribonuclease activity.

Molecular Function

Endonuclease, Hydrolase, Nuclease

Subcellular Location

Nuclease, Cytoplasm

Expression

Expressed ubiquitously

Structure

The N-terminal domain has the RNA-binding Sm fold. It may harbor the endoribonuclease activity (Potential).  Belongs to the eukaryotic release factor 1 family. Pelota subfamily.

Alternative Nomenclature

CGI 17 antibody

pelo antibody

pelota (Drosophila) homolog antibody

pelota homolog antibody

PRO1770 antibody

Protein pelota homolog antibody

General Information

Product

SIRT3 Antibody

Description

Affinity Purified NAD-dependent protein deacetylase sirtuin-3, mitochondrial Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide:

RDLVGPFVLSPRRKDVVQL

corresponding to amino acids 203-221 of rat SIRT3 protein.

Accession #

NCBI:  NP_001099783

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.54-0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

B2RZ31

Molecular Function

enzyme binding, NAD+ binding, NAD-dependent histone deacetylase activity (H3-K14 specific)

Alternative Nomenclature

Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody

NAD dependent deacetylase sirtuin 3 mitochondrial antibody

Regulatory protein SIR2 homolog 3 antibody

Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody

SIR 2 like protein 3 antibody

SIR 3 antibody

SIR2 L3 antibody

Sirtuin 3 antibody

Sirtuin type 3 antibody