General Information

Product

Cyclin D1 Antibody Affinity Purified

Description

Affinity Purified G1/S-specific cyclin-D1 Antibody C-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid regions 170-220 on human Cyclin D1 protein.

Accession #

GenBank: AAH23620.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P24385

Overview

Regulatory component of the cyclin D1-CDK4 complex that phosphorylates and inhibits members of retinoblastoma protein family including RB1 and regulates the cell-cycle during G1/S transition.

Molecular Function

Cell cycle; Cell division; DNA damage; Transcription regulation

Subcellular Location

Cytoplasm; Membrane; Nucleus

Structure

Belongs to the Cyclin D subfamily.  Contains 1 cyclin N-terminal domain

Alternative Nomenclature

B cell CLL/lymphoma 1 antibody

CCND 1 antibody

Cyl 1 antibody

G1/S specific cyclin D1 antibody

Parathyroid adenomatosis 1 antibody

PRAD1 antibody

U21B31 antibody

General Information

Product

Cyclin D1 Antibody

Description

Affinity Purified G1/S-specific cyclin-D1 Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide corresponding to amino acid sequences 213-232 on human Cyclin D1 protein.

Accession #

GenBank: AAH23620.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P24385

Overview

Regulatory component of the cyclin D1-CDK4 complex that phosphorylates and inhibits members of retinoblastoma protein family including RB1 and regulates the cell-cycle during G1/S transition.

Molecular Function

Cell cycle; Cell division; DNA damage; Transcription regulation

Subcellular Location

Cytoplasm; Membrane; Nucleus

Structure

Belongs to the Cyclin D subfamily.  Contains 1 cyclin N-terminal domain

Alternative Nomenclature

B cell CLL/lymphoma 1 antibody

CCND 1 antibody

Cyl 1 antibody

G1/S specific cyclin D1 antibody

Parathyroid adenomatosis 1 antibody

PRAD1 antibody

U21B31 antibody

General Information

Product

Cyclin D1 Antibody Affinity Purified

Description

Affinity Purified G1/S-specific cyclin-D1 Antibody Mid-Region

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide corresponding to amino acid sequences 268-295 on human Cyclin D1 protein.

Accession #

GenBank: AAH23620.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P24385

Overview

Regulatory component of the cyclin D1-CDK4 complex that phosphorylates and inhibits members of retinoblastoma protein family including RB1 and regulates the cell-cycle during G1/S transition.

Molecular Function

Cell cycle; Cell division; DNA damage; Transcription regulation

Subcellular Location

Cytoplasm; Membrane; Nucleus

Structure

Belongs to the Cyclin D subfamily.  Contains 1 cyclin N-terminal domain

Alternative Nomenclature

B cell CLL/lymphoma 1 antibody

CCND 1 antibody

Cyl 1 antibody

G1/S specific cyclin D1 antibody

Parathyroid adenomatosis 1 antibody

PRAD1 antibody

U21B31 antibody

General Information

Product

Cyclin D1 Antibody Affinity Purified

Description

Affinity Purified G1/S-specific cyclin-D1 Antibody C-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide corresponding to amino acid sequences 280-295 on human Cyclin D1 protein.

Accession #

GenBank: AAH23620.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P24385

Overview

Regulatory component of the cyclin D1-CDK4 complex that phosphorylates and inhibits members of retinoblastoma protein family including RB1 and regulates the cell-cycle during G1/S transition.

Molecular Function

Cell cycle; Cell division; DNA damage; Transcription regulation

Subcellular Location

Cytoplasm; Membrane; Nucleus

Structure

Belongs to the Cyclin D subfamily.  Contains 1 cyclin N-terminal domain

Alternative Nomenclature

B cell CLL/lymphoma 1 antibody

CCND 1 antibody

Cyl 1 antibody

G1/S specific cyclin D1 antibody

Parathyroid adenomatosis 1 antibody

PRAD1 antibody

U21B31 antibody

General Information

Product

Ki67 Antibody

Description

Affinity Purified Antigen KI-67 isoform 1 Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 2950-3000 on human antigen Ki67 isoform 1.

Accession #

NCBI:  NP_002408.3

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P46013

Overview

required for maintaining cell proliferation

Molecular Function

poly(A) RNA binding;protein C-terminus binding;ATP binding

Subcellular Location

Chromosome, Nucleus

Expression

Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.

Alternative Nomenclature

Antigen identified by monoclonal antibody Ki 67 antibody

KIA antibody

MKI67 antibody

Proliferation related Ki 67 antigen antibody

RP11-380J17.2 antibody

General Information

Product

Ki67 Antibody

Description

Affinity Purified Antigen KI-67 isoform 1 Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 1250-1300 on human antigen Ki67 isoform 1.

Accession #

NCBI:  NP_002408.3

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-region epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P46013

Overview

required for maintaining cell proliferation

Molecular Function

poly(A) RNA binding; protein C-terminus binding; ATP binding

Subcellular Location

Chromosome, Nucleus

Expression

Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.

Alternative Nomenclature

Antigen identified by monoclonal antibody Ki 67 antibody

KIA antibody

MKI67 antibody

Proliferation related Ki 67 antigen antibody

RP11-380J17.2 antibody

General Information

Product

p27 KIP 1 Antibody

Description

Affinity Purified Cyclin-dependent kinase inhibitor p27kip1 Antibody N-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Immunogen

N-epitope synthetic peptide corresponding to unique amino acid sequences on human p27 KIP1 protein.

Accession #

GenBank: AAF69497.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:250

Protein

Uniprot #

Q9NYG6

Overview

cyclin-dependent protein serine/threonine kinase inhibitor activity;plays a role in cell cycle arrest

Molecular Function

Cyclin;Kinase;Transferase

Subcellular Location

Nucleus

Alternative Nomenclature

AA408329 antibody

AI843786 antibody

CDKN 1B antibody

CDKN 4 antibody

Cyclin dependent kinase inhibitor p27 antibody

KIP 1 antibody

MEN1B antibody

MEN4 antibody

OTTHUMP00000195098 antibody

p27 antibody

General Information

Product

CDKKN2A / p14ARF Antibody 

Description

Affinity Purified partial tumor supressor p14ARF Antibody Mid-Region

Verified Applications

ELISA, WB

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Mid-Region epitope synthetic peptide corresponding to unique amino acid sequence on p14ARF protein

Accession #

GenBank: AAC6069.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00-1.25 mg/ml µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8N726

Overview

Functions as a tumor supressor and has the ability to induce cell cycle arrest in the G1 and G2 phases.  Serves to induce G2 arrest and apoptosis in a p53-independent manner via preventing the activation of cyclin B1/CDC2 complexes.

Molecular Function

DNA binding, RNA binding, p53 binding, MDM2/MDM4 family protein binding

Subcellular Location

Nucleus

Structure

No interaction with cyclins, CDK1, CDK2, CDK4, CDK5 or CDK6.  Interacts with TBRG1, COMMD1, CDKN2AIP and E4F1.  Binds to BCL6, E2F1, HUWE1, MDM2, MYC, NPM1/B23, TOP1/TOPOI and UBE2I/UBC9.

Alternative Nomenclature

ARF antibody

CDK4 inhibitor p16 INK4 antibody

CDK4I antibody

CDKN 2A antibody

Cell cycle negative regulator beta antibody

CMM2 antibody

Cyclin dependent kinase 4 inhibitor A antibody

1/2/3 antibody

Cyclin dependent kinase inhibitor p16 antibody

INK4 antibody

Melanoma p16 inhibits CDK4 antibody

MLM antibody

MTS 1 antibody

p14 antibody

p16 antibody

p19 antibody

P19ARF antibody

TP16 antibody

General Information

Product

CDKKN2A / p14ARF Antibody

Description

Affinity Purified partial tumor supressor p14ARF Antibody C-epitope

Verified Applications

ELISA, WB

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to C-terminal amino acids 94-111 of Human p14ARF.

Sequence:

QRLGQQPLPRRPGHDDGQ

Accession #

GenBank: AAC6069.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00-1.25 mg/ml µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8N726

Overview

Functions as a tumor supressor and has the ability to induce cell cycle arrest in the G1 and G2 phases.  Serves to induce G2 arrest and apoptosis in a p53-independent manner via preventing the activation of cyclin B1/CDC2 complexes.

Molecular Function

DNA binding, RNA binding, p53 binding, MDM2/MDM4 family protein binding

Subcellular Location

Nucleus

Structure

No interaction with cyclins, CDK1, CDK2, CDK4, CDK5 or CDK6.  Interacts with TBRG1, COMMD1, CDKN2AIP and E4F1.  Binds to BCL6, E2F1, HUWE1, MDM2, MYC, NPM1/B23, TOP1/TOPOI and UBE2I/UBC9.

Alternative Nomenclature

ARF antibody

CDK4 inhibitor p16 INK4 antibody

CDK4I antibody

CDKN 2A antibody

Cell cycle negative regulator beta antibody

CMM2 antibody

Cyclin dependent kinase 4 inhibitor A antibody

1/2/3 antibody

Cyclin dependent kinase inhibitor p16 antibody

INK4 antibody

Melanoma p16 inhibits CDK4 antibody

MLM antibody

MTS 1 antibody

p14 antibody

p16 antibody

p19 antibody

P19ARF antibody

TP16 antibody

General Information

Product

CDKN2A / p14ARF Antibody

Description

Affinity Purified partial tumor supressor p14ARF Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

N-epitope synthetic peptide corresponding to unique amino acid sequence on p14ARF protein

Accession #

GenBank: AAC6069.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00-1.25 mg/ml µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8N726

Overview

Functions as a tumor supressor and has the ability to induce cell cycle arrest in the G1 and G2 phases.  Serves to induce G2 arrest and apoptosis in a p53-independent manner via preventing the activation of cyclin B1/CDC2 complexes.

Molecular Function

DNA binding, RNA binding, p53 binding, MDM2/MDM4 family protein binding

Subcellular Location

Nucleus

Structure

No interaction with cyclins, CDK1, CDK2, CDK4, CDK5 or CDK6.  Interacts with TBRG1, COMMD1, CDKN2AIP and E4F1.  Binds to BCL6, E2F1, HUWE1, MDM2, MYC, NPM1/B23, TOP1/TOPOI and UBE2I/UBC9.

Alternative Nomenclature

ARF antibody

CDK4 inhibitor p16 INK4 antibody

CDK4I antibody

CDKN 2A antibody

Cell cycle negative regulator beta antibody

CMM2 antibody

Cyclin dependent kinase 4 inhibitor A antibody

1/2/3 antibody

Cyclin dependent kinase inhibitor p16 antibody

INK4 antibody

Melanoma p16 inhibits CDK4 antibody

MLM antibody

MTS 1 antibody

p14 antibody

p16 antibody

p19 antibody

P19ARF antibody

TP16 antibody