General Information

Product

NT5C2 Antibody

Description

Affinity Purified 5'-nucleotidase, cytosolic II Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique aino acid sequence on human cNII protein.

Accession #

GenBank: CAI40080

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-0.95 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P49902

Overview

May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.

Molecular Function

Magnesium, Metal-binding, Nucleotide-binding

Subcellular Location

Cytoplasm

Structure

Homotetramer

Alternative Nomenclature

5' nucleotidase (purine) cytosolic type B antibody

cN II antibody

Cytosolic 5''-nucleotidase II antibody

Cytosolic purine 5''-nucleotidase antibody

GMP antibody

NT5B antibody

nt5c2 antibody

NT5CP antibody

NUCLEOTIDASE, 5-PRIME, CYTOSOLIC II antibody

PNT5 antibody

PURINE 5-PRIME-NUCLEOTIDASE antibody

General Information

Product

Cannabinoid Receptor II Antibody

Description

Affinity Purified Cannabinoid receptor 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-350 on mouse CB2 Receptor protein common to both CB2L and CB2S.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.61-0.63 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P47936

Overview

Heterotrimeric GPCR for endocannabinoid 2-arachidonoylglycerol mediating inhibition of Adenylate cyclase.  Functions in inflammatory response, nociceptive transmission and bone homeostasis.

Molecular Function

Inflammatory response; GPCR; Receptor; Transducer

Subcellular Location

Cell membrane; Multi-pass membrane protein; Dendrite; Perikaryon

Expression

Cells of hematopoietic origin.  Expressed in skin in suprabasal layers and hair follices.  In brain by neurons and glial cells.  Expressed in osteoblasts, osteocytes and osteoclasts.

Structure

belongs to G-protein coupled receptor 1 family

Alternative Nomenclature

Cannabinoid receptor 2 macrophage antibody

CB 2 antibody

CNR2 antibody

CX 5 antibody

testis-dominant CNR2 isoform CB2 antibody

General Information

Product

PDE4 Antibody

Description

Phosphodiesterase 4 Antibody

Verified Applications

ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide common to most PDE4 subtypes: PDE4A, PDE4D, PDE4B

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

Dot Blot

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500-1:1,000

Protein

Overview

Phosphodiesterase specific for extracellular cAMP. Involved in the degradation of extracellular cAMP specifically during multicellular development.

Molecular Function

3',5'-cyclic-nucleotide phosphodiesterase activity; cAMP catabolic process; signal transduction; Hydrolase

Subcellular Location

Cell membrane; Multipass membrane protein

Expression

Low expression during growth and cell aggregation (up to 6 hours), maximally increases after 9 hours of starvation, during the mound stage. Remains expressed at significant levels during the other stages of multicellular development.

Structure

Belongs to the cyclic nucleotide phosphodiesterase family.

Alternative Nomenclature

PDE4A antibody

PDE4B antibody

PDE4C antibody

PDE4D antibody

Phosphodiesterase 4 antibody

Phosphodiesterase 4A antibody

Phosphodiesterase 4B antibody

Phosphodiesterase 4C antibody

Phosphodiesterase 4D antibody

General Information

Product

Phospho NT5C2 Antibody Affinity Purified

Description

Affinity Purified Phospho 5'-nucleotidase, cytosolic II Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequences on cNII protein with phosphorylated Serine 7.

Accession #

GenBank: CAI40080

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-0.85 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P49902

Overview

May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.

Molecular Function

Magnesium, Metal-binding, Nucleotide-binding

Subcellular Location

Cytoplasm

Structure

Homotetramer

Alternative Nomenclature

5' nucleotidase (purine) cytosolic type B antibody

cN II antibody

Cytosolic 5''-nucleotidase II antibody

Cytosolic IMP/GMP-specific 5'-nucleotidase antibody

GMP antibody

NT5B antibody

NT5CP antibody

NUCLEOTIDASE, 5-PRIME, TYPE B antibody

PNT5 antibody

PURINE 5-PRIME-NUCLEOTIDASE antibody

General Information

Product

PDE4C Antibody

Description

Affinity Purified cAMP-specific 3',5'-cyclic phosphodiesterase 4C Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic cyclic carboxy-terminal peptide common to all PDE4C variants.

Accession #

NCBI:  NP_001074.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500-1:2,500

Protein

Uniprot #

Q08493

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Function

Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Subcellular Location

Cell projection > cillium

Expression

Expressed in various tissues but not in cells of the immune system.

Structure

Part of a complex containing AKAP5, ADCY5, ADCY6 and PKD2.

Alternative Nomenclature

5''-cyclic phosphodiesterase 4C antibody

cAMP specific 3' 5' cyclic phosphodiesterase 4C antibody

cAMP-specific 3'' antibody

DPDE 1 antibody

MGC126222 antibody

PDE 21 antibody

PDE 4C antibody

Pde4c antibody

Phosphodiesterase 4C antibody

Phosphodiesterase E1 dunce homolog antibody

Phosphodiesterase type 4C antibody

General Information

Product

PGCB Antibody

Description

Affinity Purified Atrial natriuretic peptide receptor 2 Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic C-terminal peptide corresponding to unique amino acid sequences on Particulate Guanylyl Cyclase B protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-1.20 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:50-1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P20594

Overview

Receptor for the C-type natriuretic peptide NPPC/CNP hormone. Has guanylate cyclase activity upon binding of its ligand. May play a role in the regulation of skeletal growth.

Molecular Function

ATP binding, GTP binding, hormone binding, Protein kinase activity, G-protein coupled peptide receptor activity

Subcellular Location

Membrane; Single-pass type I membrane protein

Structure

Belongs to the adenylyl cyclase-class-4/guanylyl cyclase family.  Contains 1 guanylate cyclase domain.  Contains 1 protein kinase domain.

Alternative Nomenclature

AMDM antibody

ANPB antibody

ANPRB antibody

peptide receptor type B antibody

Atrionatriuretic peptide receptor B antibody

GC-B antibody

GUCY2B antibody

Natriuretic peptide receptor B/guanylate cyclase B antibody

NPR-B antibody

NPR2 antibody

NPRBi antibody

General Information

Product

Adenylate Cyclase 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rabbit, Rat

Immunogen

Synthetic peptide corresponding to conserved sequence at or near C terminal end of protein that is unique to rat/mouse Adenylyl Cyclase 1 protein.

Accession #

GenBank: AAC29478 

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55- 0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500. Detects a band 127-10 kDa

Protein

Uniprot #

O88444

Overview

This is a calmodulin-sensitive adenylyl cyclase. May be involved in regulatory processes in the central nervous system. It may play a role in memory acquisition and learning. Plays a role in the regulation of the circadian rhythm of daytime contrast sensitivity probably by modulating the rhythmic synthesis of cyclic AMP in the retina

Molecular Function

ATP-binding, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Subcellular Location

Membrane; Multi-pass membrane protein. Cytoplasm

Expression

Expressed throughout inner ear development. Brain, retina and adrenal medulla.

Structure

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 2 guanylate cyclase domains

Alternative Nomenclature

3'5' cyclic AMP synthetase antibody

ADCY1 antibody

adenyl cyclase antibody

Adenylate cyclase 1 antibody

ATP pyrophosphate lyase antibody

Brain adenylate cyclase 1 antibody

Ca(2+)/calmodulin activated adenylyl cyclase antibody

EC 4.6.1.1 antibody

General Information

Product

ADCY2 Antibody

Description

Affinity Purified Particulate Adenylate cyclase type 2 Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence from rat Particulate Adenylyl Cyclase 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Western Blot

1:1,000

Protein

Uniprot #

P26769

Overview

Membrane-bound calmodulin-insensitive adenylyl cyclase.

Molecular Function

Adenylate cyclase activity; cAMP biosynthesis; ATP-binding; Magnesium; Metal binding; Nucleotide-binding

Subcellular Location

Membrane; Multi-pass membrane protein

Expression

Brain and in lower levels, the olfactory epithelium and lung.

Structure

Interacts with RAF1

Alternative Nomenclature

3',5' cyclic AMP synthetase antibody

Adenylate cyclase II antibody

AC2 antibody

Adenylate cyclase 2 (brain) antibody

ATP pyrophosphate lyase 2 antibody

FLJ16822 antibody

FLJ45092 antibody

HBAC2 antibody

KIAA1060 antibody

MGC133314 antibody

Type II adenylate cyclase antibody

General Information

Product

ADCY3 Antibody

Description

Particulate Adenylate cyclase type 3 Antibody Affinity Purified

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Mouse, Rat

Immunogen

Synthetic peptide generated against a sequence unique to PAC3 protein. All peptides were amidated before conjugation.

Accession #

NCBI:  NP_004027

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Polyclonal

Clonality

Rabbit

Determinant

Affinity Purified

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O60266

Overview

Mediates odorant detection via modulation of intracellular cAMP concentration

Molecular Function

Adenylate cyclase activity; cAMP biosynthesis; ATP-binding; Magnesium; Metal binding; Nucleotide-binding

Subcellular Location

Membrane; Multi-pass membrane protein

Expression

Brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle

Structure

Belongs to adenylyl cyclase class-4 and guanylyl cyclase family.  Contains 2 guanylate cyclase domains.

Alternative Nomenclature

ATP pyrophosphate-lyase 3 antibody

olfactive type antibody

General Information

Product

Adenylate Cyclase 4 Antibody

Description

Affinity Purified Adenylate cyclase type 4 Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on rat Particulate Adenylyl Cyclase 4 protein.

Accession #

NCBI:  NP_062158

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.526 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100-1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100-1:200

Immunofluorescence

1:100-1:200

Immunohistochemistry

1:100-1:200

Western Blot

1:500

Protein

Uniprot #

Q66HK5

Molecular Function

Nucleotide-binding

Subcellular Location

Membrane

Structure

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family

Alternative Nomenclature

AC4 antibody

Adcy4 antibody

Adenylate cyclase 4 antibody

ATP pyrophosphate-lyase 4 antibody