General Information
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Product
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TrkA Peptide
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Description
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Antigenic Blocking Peptide Tyrosine Receptor Kinase A
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Verified Applications
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Immunocompetition, Immunodepletion
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Peptide Description
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Synthetic peptide taken within amino acid region 360-410 on human TrkA protein.
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Accession #
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GenBank: BAA34355.1
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Physical Properties
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Quantity
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250 µg
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Volume
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100 µl
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Form
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Antigenic Blocking Peptide
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Storage
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-20⁰C for long term storage
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Application Protocol
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Immunohistochemistry |
1:100 |
Competition/Depletion assay
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1:500
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Protein
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Uniprot #
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P04629
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Overview
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Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors. Isoform TrkA-III is resistant to NGF, constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.
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Molecular Function
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Developmental Protein; Kinase; Receptor; Transferase; Tyrosine-protein kinase
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Subcellular Location
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Cell membrane; Single-pass type I membrane protein; Early & Late endosome membrane
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Expression
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Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.
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Structure
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Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 2 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 protein kinase domain.
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Alternative Nomenclature
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CIPA antibody
gp140trk antibody
High affinity nerve growth factor receptor antibody
MTC antibody
Neurotrophic tyrosine kinase receptor type 1 antibody
Ntrk1 antibody
Oncogene TRK antibody
p140-TrkA antibody
Slow nerve growth antibody
TRK antibody
Trk-A antibody
TRK1 antibody
TRK1-transforming tyrosine kinase protein antibody
Tropomyosin-related kinase A antibody
Tyrosine kinase receptor A antibody
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