General Information

Product

D6 Antibody

Description

Affinity Purified Atypical chemokine receptor 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-378 mouse Atypical chemokine receptor 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O08707

Overview

Controls chemokine levels and localization via high-affinity chemoking binding that is uncoupled from classic liand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis.  Acts as a regulator of inflammatory leukocyte interactions with lymphatic endothelial cells and is required for immature/mature dendritic cells discrimination.

Molecular Function

Chemokine Receptor activity; Scavenger receptor activity

Subcellular Location

Early endosome; Recycling endosome; Cell membrane; Multi-pass membrane protein

Expression

Endothelial cells lining afferent lympatics in dermis and lymph nodes.  Also found n subcapsular and medullary sinuses, tonsillar lympatic sinuses and lymphatics in mucosa and submucosa of appendix, small intestine, and large intestine.

Structure

C-terminal cytoplasmic tail controls phosphorylation, stability, intracellular trafficking and chemokine scavenging properties.

Alternative Nomenclature

ACKR2 antibody

Atypical chemokine receptor 2 antibody

Chemokine binding protein 2 antibody

Chemokine receptor CCR 10 antibody

Chemokine receptor CCR 9 antibody

CMKBR9 antibody

General Information

Product

D6 Antibody 

Description

Affinity Purified Atypical chemokine receptor 2 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 200-300 on ACRD6 protein.

Accession #

NCBI:  NP_001287.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O00590

Overview

Controls chemokine levels and localization via high-affinity chemoking binding that is uncoupled from classic liand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis.  Acts as a regulator of inflammatory leukocyte interactions with lymphatic endothelial cells and is required for immature/mature dendritic cells discrimination.

Molecular Function

Chemokine Receptor activity; Scavenger receptor activity

Subcellular Location

Early endosome; Recycling endosome; Cell membrane; Multi-pass membrane protein

Expression

Endothelial cells lining afferent lympatics in dermis and lymph nodes.  Also found n subcapsular and medullary sinuses, tonsillar lympatic sinuses and lymphatics in mucosa and submucosa of appendix, small intestine, and large intestine.  Experssed in Kapo

Structure

C-terminal cytoplasmic tail controls phosphorylation, stability, intracellular trafficking and chemokine scavenging properties.

Alternative Nomenclature

ACKR2 antibody

Atypical chemokine receptor 2 antibody

Chemokine binding protein 2 antibody

Chemokine receptor CCR 10 antibody

Chemokine receptor CCR 9 antibody

CMKBR9 antibody

General Information

Product

CXCR1 Antibody

Description

Affinity Purified CXCR1 Antibody N-epitope

Verified Applications

ELISA, IHC-P, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide derived from the N terminus of Human CXCR1 protein.

Accession #

GenBank: AAY21523

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:250

Western Blot

1:500. Predicted molecular weight: 40 kDa.

Protein

Uniprot #

P25024

Overview

Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity.

Molecular Function

Chemotaxis

Subcellular Location

Cell membrane; Multi-pass membrane protein

Structure

Belongs to the G-protein coupled receptor 1 family

Alternative Nomenclature

C C antibody

C C CKR 1 antibody

C-X-C chemokine receptor type 1 antibody

CCCKR1 antibody

CD 181 antibody

CD128 antibody

CKR 1 antibody

CMKAR1 antibody

CXC-R1 antibody

High affinity interleukin-8 receptor A antibody

IL 8 receptor type 1 antibody

IL 8R A antibody

General Information

Product

CXCR1 Antibody

Description

Affinity Purified IL-8 receptor type A Antibody C-epitope

Verified Applications

ELISA, ICC/IF, IHC-P, IP

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide, corresponding to a region within C terminal amino acids 272-350 of Human CXCR1.

Accession #

GenBank: BAA92293.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

5-.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:250

Western Blot

1:500. Predicted molecular weight: 40 kDa.

Protein

Uniprot #

P25024

Overview

Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity.

Molecular Function

Chemotaxis

Subcellular Location

Cell membrane; Multi-pass membrane protein

Structure

Belongs to the G-protein coupled receptor 1 family

Alternative Nomenclature

C C antibody

C C CKR 1 antibody

C-X-C chemokine receptor type 1 antibody

CCCKR1 antibody

CD 181 antibody

CD128 antibody

CKR 1 antibody

CMKAR1 antibody

CXC-R1 antibody

High affinity interleukin-8 receptor A antibody

IL 8 receptor type 1 antibody

IL 8R A antibody

General Information

Product

CX3CL1 Antibody

Description

Affinity Purified Fractalkine Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid reigon 350-395 on mouse Fractalkine protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O35188

Overview

Acts as a ligand for both CX3CL1 and integrins. Binds to CX3CR1 and to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium (PubMed:9177350, PubMed:10382755).

Molecular Function

CCR chemokine receptor binding, chemokine activity, CX3C chemokine receptor binding, integrin binding

Subcellular Location

Cell membrane, Single-pass type I membrane protein, Processed fractalkine : Secreted

Expression

Highest levels in brain. Lower levels in kidney, heart and lung. Also found in skeletal muscle and testis.

Structure

Signal, Transmembrane, Transmembrane helix

Alternative Nomenclature

A 152E5.2 antibody

AB030188 antibody

ABCD3 antibody

AI848747 antibody

C-X3-C motif chemokine 1 antibody

C3Xkine antibody

Chemokine C X3 C motif ligand 1 antibody

CX3C membrane anchored chemokine antibody

Cx3cl1 antibody

CXC3 antibody

CXC3C antibody

D8Bwg0439e antibody

FKN antibody

Fractalkine antibody

Neurotactin antibody

NTN antibody

NTT antibody

Processed fractalkine antibody

SCYD 1 antibody

Small inducible cytokine D1 antibody

X3CL1_HUMAN antibody