General Information

Product

Sall4C Antibody

Description

Affinity Purified SALL4C Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Mouse, Pig, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human SALL4C protein.

Accession #

GenBank: AAO16567.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q6Y8G5

Overview

Transcription factor with a key role in the maintenance and self-renewal of embryonic and hematopoietic stem cells.

Molecular Function

DNA binding; Metal ion binding; Transcription Regulation

Subcellular Location

Cytoplasm, Nucleus

Structure

Belongs to the sal C2H2-type zinc-finger protein family.

Contains 7 C2H2-type zinc fingers.

Alternative Nomenclature

AA407717 antibody

AL022809 antibody

AW536104 antibody

C330011P20Rik antibody

C78083 antibody

C78563 antibody

DRRS antibody

HSAL4 antibody

Sal-like Protein 4 antibody

Spalt like transcription factor 4 antibody

Tex20 antibody

Zinc finger protein 797 antibody

Zinc finger protein SALL4 antibody

ZNF797 antibody

General Information

Product

SGK1 Antibody

Description

Affinity Purified Serine/Threonine-protein Kinase Sgk1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide taken within amino acid region 40-90 on human SGK1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O00141

Overview

Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cellular enzymes, transcription factors, neuronal excitability, cell growth, proliferation, survival, migration and apoptosis. Plays an important role in cellular stress response. Contributes to regulation of renal Na+ retention, renal K+ elimination, salt appetite, gastric acid secretion, intestinal Na+/H+ exchange and nutrient transport, insulin-dependent salt sensitivity of blood pressure, salt sensitivity of peripheral glucose uptake, cardiac repolarization and memory consolidation. Up-regulates Na+ channels: SCNN1A/ENAC, SCN5A and ASIC1/ACCN2, K+ channels: KCNJ1/ROMK1, KCNA1-5, KCNQ1-5 and KCNE1, epithelial Ca2+ channels: TRPV5 and TRPV6, chloride channels: BSND, CLCN2 and CFTR, glutamate transporters: SLC1A3/EAAT1, SLC1A2 /EAAT2, SLC1A1/EAAT3, SLC1A6/EAAT4 and SLC1A7/EAAT5, amino acid transporters: SLC1A5/ASCT2, SLC38A1/SN1 and SLC6A19, creatine transporter: SLC6A8, Na+/dicarboxylate cotransporter: SLC13A2/NADC1, Na+-dependent phosphate cotransporter: SLC34A2/NAPI-2B, glutamate receptor: GRIK2/GLUR6. Up-regulates carriers: SLC9A3/NHE3, SLC12A1/NKCC2, SLC12A3/NCC, SLC5A3/SMIT, SLC2A1/GLUT1, SLC5A1/SGLT1 and SLC15A2/PEPT2. Regulates enzymes: GSK3A/B, PMM2 and Na+/K+ ATPase, and transcription factors: CTNNB1 and nuclear factor NF-kappa-B. Stimulates sodium transport into epithelial cells by enhancing the stability and expression of SCNN1A/ENAC. This is achieved by phosphorylating the NEDD4L ubiquitin E3 ligase, promoting its interaction with 14-3-3 proteins, thereby preventing it from binding to SCNN1A/ENAC and targeting it for degradation. Regulates store-operated Ca(+2) entry (SOCE) by stimulating ORAI1 and STIM1.

Molecular Function

ATP-binding, Nucleotide binding; Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm; Nucleus; Endoplasmic reticulum membrane; Cell membrane; Mitochondrion

Expression

Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.

Structure

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 protein kinase domain.

Alternative Nomenclature

OTTHUMP00000017247 antibody
Serine/threonine protein kinase Sgk1 antibody
Serum and glucocorticoid regulated kinase antibody
Serum/glucocorticoid-regulated kinase 1 antibody
SGK 1 antibody
SGK antibody
Sgk1 variant i3 antibody

General Information

Product

IFITM3 Antibody

Description

Affinity Purified Interferon-induced transmembrane protein 3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human Interferon-induced transmembrane protein 3.

Accession #

GenBank: AFF60350

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q01628

Overview

IFN-induced antiviral protein which disrupts intracellular cholesterol homeostasis. Inhibits the entry of viruses to the host cell cytoplasm by preventing viral fusion with cholesterol depleted endosomes. May inactivate new enveloped viruses which buds out of the infected cell, by letting them go out with a cholesterol depleted membrane. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV) and Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and vesicular stomatitis virus (VSV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV S protein-mediated viral entry and VSV G protein-mediated viral entry. Plays a critical role in the structural stability and function of vacuolar ATPase (v-ATPase). Establishes physical contact with the v-ATPase of endosomes which is critical for proper clathrin localization and is also required for the function of the v-ATPase to lower the pH in phagocytic endosomes thus establishing an antiviral state.

Molecular Function

Antiviral defense, Immunity, Innate immunity

Subcellular Location

Cell membrane; Single-pass type II membrane protein

Late endosome membrane; Single-pass type II membrane protein

Lysosome membrane; Single-pass type II membrane protein

Expression

Induced by IFN-alpha and IFNG/IFN-gamma.

Structure

Interacts with ATP6V0B and CD81. Interacts with SPP1; the interaction reduces OPN expression. Interacts with VAPA.

Alternative Nomenclature

1 8U 

Fragilis 

IFITM3 

Interferon Induced Transmembrane Protein 3

Interferon inducible 

Interferon inducible protein 1 8U 

Interferon Inducible Protein 15 

Interferon Inducible Protein Homolog 

IP15