General Information
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Product
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Phospho-PDE5A Antibody
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Description
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Affinity Purified Phosphorylated Phosphodiesterase 5A Antibody
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Verified Applications
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ELISA, ICC, IHC, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Bovine, Human, Mouse, Pig, Rat, Sheep
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Immunogen
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Synthetic peptide common to all PDE5A variants taken within amino acid region 120-170 on human PDE5A protein. Phosphoryalted
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Specificity |
This antibody will detect only PDE5A protein that has been phosphorylated at the PKG site. Will not detect unphosphorylated PDE5A, or other PDE family members. |
Accession #
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GenBank: EAX05277.1
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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1.0-1.25 µg/µl in antibody stabilization buffer
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Form
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Affinity Purified
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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Confocal Microscopy
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1:100
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DB
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1:10,000
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ELISA
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1:10,000
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Immunocytochemistry
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1:100
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Immunofluorescence
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1:100
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Immunohistochemistry
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1:100
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Immunoprecipitation
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1:200
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Western Blot
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1:500
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Protein
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Uniprot #
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UPI0000EE2B22
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Overview
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Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.
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Molecular Function
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Hydrolase, 3',5'-cyclic-GMP phosphodiesterase activity, cGMP binding
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Subcellular Location
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cytosol
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Expression
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Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
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Alternative Nomenclature
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5''-cyclic phosphodiesterase antibody
CGB PDE antibody
cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody
cGMP binding/cGMP specific phosphodiesterase antibody
cGMP specific phosphodiesterase type 5A antibody
CN5A antibody
CN5N antibody
PDE 5 antibody
PDE 5A antibody
PDE5A1 antibody
Phosphodiesterase 5A antibody
Phosphodiesterase 5A cGMP specific antibody
Phosphodiesterase isozyme 5 antibody
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