11 - 20 of 47 results

PDE2A Antibody

Product no.: PD2A-112AP

$320.00

Description
Publications

General Information
Product	PDE2A Antibody
Description	Affinity Purified Phosphodiesterase 2A, cGMP-stimulated Antibody N-epitope
Verified Applications	CM, ELISA, ICC/IF, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Bovine, Human, Mouse, Rat
Immunogen	Synthetic peptide corresponding to unique amino acid sequence on PDE2A gene.
Specificity	This antibody will label PDE2A1, PDE2A2, PDE2A3 and PDE2A4 variants.
Accession #	GenBank: EAW74861.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.7 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Determinant	N-epitope
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:250-1:1,000
DB	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:250-1:1,000
Immunofluorescence	1:150-1:1,000
Immunohistochemistry	1:250-1:1,000
Immunoprecipitation	1:200
Western Blot	1:250-1:1,000
Protein
Uniprot #	UPI000003B340
Overview	Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis.
Molecular Function	Hydrolase, cAMP, cGMP, Metal-binding, Angiogenesis
Subcellular Location	Multi-pass membrane protein
Expression	bundant in adipose tissues.
Structure	Interacts with PIK3CG (By similarity). Interacts with RAPGEF3 and PIK3R6.
Alternative Nomenclature	5''-cyclic phosphodiesterase antibody cGMP dependent 3'5' cyclic phosphodiesterase antibody cGMP stimulated phosphodiesterase 1 antibody cGMP stimulated phosphodiesterase 4 antibody cGMP-dependent 3'' antibody CGS PDE antibody Cyclic GMP stimulated phosphodiesterase antibody PDE 2A antibody PDE 2A1 antibody PDE 2A4 antibody PDE2A antibody Phosphodiesterase 2A cGMP stimulated antibody

Protein information supplied by UniProt

Fabgennix PDE2A Antibody has been used in 34 Publications

Petersen, T. S., et al. "Distribution and function of 3', 5'-Cyclic-AMP phosphodiesterases in the human ovary." Molecular and cellular endocrinology (2015). PMID25578602

Immunohistochemistry; Human

Kedia, George T., et al. "Expression and Distribution of Phosphodiesterase Isoenzymes in the Human Male Urethra." Urology (2015). PMID25704994

Immunohistochemistry, Western Blot; Human

Rajagopal, Senthilkumar, et al. "Cytokine-Induced S-Nitrosylation of Soluble Guanylyl Cyclase and Expression of Phosphodiesterase 1A Contribute to Dysfunction of Longitudinal Smooth Muscle Relaxation." Journal of Pharmacology and Experimental Therapeutics 352.3 (2015): 509-518. PMID25550199

Western Blot; Mouse

Rentsendorj, Otgonchimeg, Franco R. D'Alessio, and David B. Pearse. "Phosphodiesterase 2A is a major negative regulator of iNOS expression in lipopolysaccharide-treated mouse alveolar macrophages." Journal of leukocyte biology 96.5 (2014): 907-915. PMID25063878

Western Blot; Mouse

Zhai, Kui, et al. "Phosphodiesterase types 3 and 4 regulate the phasic contraction of neonatal rat bladder smooth myocytes via distinct mechanisms." Cellular signalling 26.5 (2014): 1001-1010. PMID24463006

Western Blot; Rat

Yougbaré, Issaka, et al. "Cyclic GMP catabolism up-regulation in MRL/lpr lupus-prone mice is associated with organ remodeling." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 1842.7 (2014): 916-926. PMID24631654

Immunohistochemistry, Western Blot; Mouse

Hiramoto, Kenichi, et al. "Role of phosphodiesterase 2 in growth and invasion of human malignant melanoma cells." Cellular signalling 26.9 (2014): 1807-1817. PMID24705027

Western Blot; Human

MORITA, HIROSHI et al. “Characterization of Phosphodiesterase 2A in Human Malignant Melanoma PMP Cells.” Oncology Reports 29.4 (2013): 1275–1284. PMC3621658

Western Blot; Human

Brechbühl, Julien, Fabian Moine, and Marie-Christine Broillet. “Mouse Grueneberg Ganglion Neurons Share Molecular and Functional Features withC. Elegans Amphid Neurons.” Frontiers in Behavioral Neuroscience 7 (2013): 193. PMC3856774

Immunohistochemistry; Mouse

Matsuo, Tomohiko, et al. "The wiring of Grueneberg ganglion axons is dependent on neuropilin 1." Development 139.15 (2012): 2783-2791. PMID22745317

Immunohistochemistry; Mouse

For a complete listing of FabGennix PDE2A publications, please click here.

Publishing research using PD2A-101AP? Please let us know, we can cite the reference in this datasheet.

Accessories

Product

Note

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PDE2A Antibody FITC

PD2A.n-FITC

$375.00 *

PDE2A Antibody BIOTIN

PD2A.n-BIOTIN

$375.00 *

PDE2A Blocking Peptide

P-PD2A.n

$155.00 *

PDE2A Positive Control

PC-PD2A

$220.00 *

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PD2A-101AP PDE2A Antibody $320.00 *	PD2A1-101AP PDE2A1 Antibody $285.00 *	PD2A2-201AP PDE2A2 Antibody $285.00 *
PD2A3-301AP PDE2A3 Antibody $285.00 *	PD2A4-121AP PDE2A4 Antibody $285.00 *

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Browse these categories as well: cAMP, cGMP, Phosphodiesterases, Primaries, Metabolism & Homeostasis, Cardiology

PDE3A Antibody

Product no.: PD3A-101AP

$320.00

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General Information
Product	PDE3A Antibody
Description	Affinity Purified cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform 1 Antibody
Verified Applications	ELISA, FRET, ICC/IF, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Monkey, Mouse, Rat, Xenopus
Immunogen	Synthetic peptide selective only for PDE3A variants.
Accession #	NCBI: NP_000912.3
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.719 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:250
DB	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:250
Immunofluorescence	1:250
Immunohistochemistry	1:250
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	Q14432
Overview	Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.
Molecular Function	Hydrolase, 3', 5'-cyclic-GAP phosphodiesterase activity, cAMP binding
Subcellular Location	Membrane; Multi-pass membrane protein
Alternative Nomenclature	cAMP phosphodiesterase myocardial cGMP inhibited antibody CGI PDE A antibody CGI PDE antibody Cyclic GMP-inhibited phosphodiesterase A antibody Human cGMP inhibited cAMP phosphodiesterase mRNA antibody PDE 3A antibody Phosphodiesterase 3A antibody Phosphodiesterase 3A cGMP inhibited antibody

Protein information supplied by UniProt

FabGennix PDE3A Antibody has been used in 23 Publications

Yang, Zhaokang, et al. "The Golgi apparatus is a functionally distinct Ca2+ store regulated by the PKA and Epac branches of the β1-adrenergic signaling pathway." Sci. Signal. 8.398 (2015): PMID26462734

Western Blot; Rat

Fields, Laura A., Andreas Koschinski, and Manuela Zaccolo. "Sustained exposure to catecholamines affects cAMP/PKA compartmentalised signalling in adult rat ventricular myocytes." Cellular signalling (2015). PMID26475678

Confocal imaging, FRET, Western Blot; Rat

Western Blot; Mouse

Petersen, T. S., et al. "Distribution and function of 3', 5'-Cyclic-AMP phosphodiesterases in the human ovary." Molecular and cellular endocrinology (2015). PMID25578602

Immunocytochemistry; Human

Mahavadi, Sunila, et al. "Cytokine-induced iNOS and ERK1/2 inhibit adenylyl cyclase type 5/6 activity and stimulate phosphodiesterase 4D5 activity in intestinal longitudinal smooth muscle." American Journal of Physiology-Cell Physiology 307.4 (2014): C402-C411.PMID24944202

Western Blot; Mouse

Amanda, G. Vang, et al. "Regulatory T-cells and cAMP suppress effector T-cells independently of PKA-CREM/ICER: a potential role for Epac." Biochemical Journal 456.3 (2013): 463-473. PMID24007532

Western Blot; Mouse

MacDougall, David A. et al. “Caveolae Compartmentalise Β2-Adrenoceptor Signals by Curtailing cAMP Production and Maintaining Phosphatase Activity in the Sarcoplasmic Reticulum of the Adult Ventricular Myocyte.”Journal of Molecular and Cellular Cardiology 52.2 (2012): 388–400. PMC3270222

Western Blot; Rat

Ückert, Stefan, et al. "Expression and distribution of phosphodiesterase isoenzymes in the Human seminal vesicles." The journal of sexual medicine 8.11 (2011): 3058-3065. PMID21810184

Immunohistochemistry, Western Blot; Human

Nishioka, Kinue, et al. "Cilostazol Suppresses Angiotensin II–Induced Vasoconstriction via Protein Kinase A–Mediated Phosphorylation of the Transient Receptor Potential Canonical 6 Channel." Arteriosclerosis, thrombosis, and vascular biology 31.10 (2011): 2278-2286. PMID21799177

Western Blot; Rat

Schmidt, Eric P. et al. “Soluble Guanylyl Cyclase Contributes to Ventilator-Induced Lung Injury in Mice.” American Journal of Physiology - Lung Cellular and Molecular Physiology 295.6 (2008): L1056–L1065. PMC2604795

Western Blot; Mouse

We could not fit all of our publications on this page!

For a complete listing of FabGennix PDE3A publications, please click here.

Publishing research using PD3A-101AP? Please let us know, we can cite the reference in this datasheet.

To view the PDE3A Antibody (PD3A-101AP) specification sheet, click the link below:

FabGennix PD3A-101AP Specification Sheet

Accessories

Product

Note

Status

Price

PDE3A Antibody FITC

PD3A-FITC

$375.00 *

PDE3A Antibody BIOTIN

PD3A-BIOTIN

$375.00 *

PDE3A Blocking Peptide

P-PD3A

$155.00 *

PDE3A Positive Control

PC-PD3A

$220.00 *

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We also recommend

PPD3A-140AP

Phospho-PDE3A Antibody

$375.00 *

PD3A-112AP Goat Antibody

PDE3A Antibody

$320.00 *

PD3B-101AP

PDE3B Antibody

$320.00 *

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PDE5A Antibody

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PDE2A4 Antibody

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PDE3B Antibody

Product no.: PD3B-101AP

$320.00

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General Information
Product	PDE3B Antibody
Description	Affinity Purified cGMP-inhibited 3',5'-cyclic phosphodiesterase B Antibody
Verified Applications	CM, ELISA, ICC, IF, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Chicken, Human, Monkey, Mouse, Rat
Immunogen	Synthetic peptide taken within amino acid region 1060-1110 on human PDE3B protein.
Specificity	This antibody does not cross react with PDE4A variants nor with any other PDE famly members. This antibody will recognize PDE3B variants.
Accession #	GenBank: BAA09306
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.70 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:250
Dot Blot	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:250
Immunofluorescence	1:250
Immunohistochemistry	1:250
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	Q13370
Overview	Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Regulates cAMP binding of RAPGEF3.
Molecular Function	Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding
Subcellular Location	Membrane; Multipass membrane protein
Expression	Abundant in adipose tissues.
Structure	Interacts with PIK3CG. Interacts with RAPGEF3 and PIK3R6.
Alternative Nomenclature	5''-cyclic phosphodiesterase B antibody adipocyte cAMP phosphodiesterase cGMP inhibited antibody AI847709 antibody cAMP Phosphodiesterase, adipocyte, cGMP-inhibited antibody CGIP1 antibody CGIPDE B antibody cGMP inhibited 3 5 cyclic phosphodiesterase B antibody CGMP inhibited cAMP phophodiesterase antibody cGMP-inhibited 3'' antibody Cyclic GMP-inhibited phosphodiesterase B antibody Cyclic nucleotide phosphodiesterase antibody HcGIP1 antibody Phosphodiesterase 3B cGMP inhibited antibody

Protein information supplied by UniProt

Fabgennix PDE3B Antibody has been used in 4 Publications

Huang, Lei, et al. "Phosphoinositide 3-Kinase Gamma Contributes to Neuroinflammation in a Rat Model of Surgical Brain Injury." The Journal of Neuroscience 35.29 (2015): 10390-10401. PMC4510283

Western Blot; Rat

Petersen, T. S., et al. "Distribution and function of 3', 5'-Cyclic-AMP phosphodiesterases in the human ovary." Molecular and cellular endocrinology (2015). PMID25578602

Immunohistochemistry, Human

Dov, Avital, et al. "Diminished phosphodiesterase-8B potentiates biphasic insulin response to glucose." Endocrinology 149.2 (2008): 741-748. PMID17991719

Western Blot; Rat

Netherton, Stuart J., and Donald H. Maurice. "Vascular endothelial cell cyclic nucleotide phosphodiesterases and regulated cell migRation: implications in angiogenesis." Molecular pharmacology 67.1 (2005): 263-272. PMID15475573

Western Blot; Bovine, Human

Publishing research using PD3B-101AP? Please let us know, we can cite the reference in this datasheet.

Do you have any suggestions about the PDE3b antibody? It gives bands about 100 for different concentrations I used. I loaded 20 micrograms of cortex and cerebellum lysates and incubated the nitrocellulose membranes with the PDE1(a,b,c) and PDE3b antibodies.
1. Thank you for your inquiry about PD3B-101AP,
  I looked at the gel images you provided and am curious why you are using a high percentage or gradient gel for a protein that is above 100kDa. There are two issues that I can think of that may be affecting the results. The high percentage acrylamide may be reducing the efficiency of high MW proteins onto nitrocellulose membrane. The cortex is not the regions where we see PDE3B in high density, so it may be possible that the levels of PDE3B is already below the detection limit here. Second issue may be due to proteolysis. PDE3B is cleaved off by endogenous proteases if their activity is not checked properly. In order to curb this prepare the homogenates and samples in the presence of Fabgennix Protease Inhibitor Cocktail before the SDS-PAGE. Use PC-PDE3B to identify bands and validate the system.

Accessories

Product

Note

Status

Price

PDE3B Antibody FITC

PD3B.101-FITC

$375.00 *

PDE3B Antibody BIOTIN

PD3B.101-BIOTIN

$375.00 *

PDE3B Blocking Peptide

P-PD3B.101

$155.00 *

PDE3B Positive Control

PC-PD3B

$220.00 *

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We also recommend

PPD3A-140AP Phospho-PDE3A Antibody $375.00 *	PPD3B-140AP Phospho-PDE3B Antibody $375.00 *	PPD3B-150AP Phospho-PDE3B Antibody $375.00 *
PD3B-112AP PDE3B Antibody $320.00 *	PD3A-101AP PDE3A Antibody $320.00 *

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Browse these categories as well: cAMP, cGMP, Lipids, Metabolism, Primaries, Phosphodiesterases

PDE5A Antibody

Product no.: PD5A-101AP

$320.00

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General Information
Product	PDE5A Antibody
Description	Affinity Purified cGMP-specific 3',5'-cyclic phosphodiesterase isoform 1 Antibody
Verified Applications	CM, ELISA, ICC/IF, IHC-P, IHC-Fr, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Pig, Rat
Immunogen	Synthetic peptide common to all PDE5A variants taken within amino acid region 120-170 on human PDE5A protein.
Specificity	This antibody detects 99 and 89 kDa PDE5A proteins in various tissues. This antibody does not cross-react with other PDE family members.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.55-0.60 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:250
DB	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:250
Immunofluorescence	1:250
Immunohistochemistry	1:250
Immunoprecipitation	1:250
Western Blot	1:500-1:1,000
Protein
Uniprot #	O76074
Overview	Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.
Molecular Function	Hydrolase, 3',5'-cyclic-GMP phosphodiesterase activity, cGMP binding
Subcellular Location	cytosol
Expression	Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
Alternative Nomenclature	5''-cyclic phosphodiesterase antibody CGB PDE antibody cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody cGMP binding/cGMP specific phosphodiesterase antibody cGMP specific 3'5' cyclic phosphodiesterase antibody cGMP specific phosphodiesterase PDE5A2 antibody cGMP specific phosphodiesterase type 5A antibody cGMP-specific 3'' antibody CN5A antibody CN5N antibody PDE 5 antibody PDE 5A antibody PDE5A1 antibody Phosphodiesterase 5A antibody Phosphodiesterase 5A cGMP specific antibody Phosphodiesterase isozyme 5 antibody

Protein information supplied by UniProt

Fabgennix PDE5A Antibody has been used in 22 Publications
Rajagopal, Senthilkumar, et al. "Cytokine-Induced S-Nitrosylation of Soluble Guanylyl Cyclase and Expression of Phosphodiesterase 1A Contribute to Dysfunction of Longitudinal Smooth Muscle Relaxation." Journal of Pharmacology and Experimental Therapeutics 352.3 (2015): 509-518. PMID25704994	Immunofluorescence, Western Blot; Human
Kedia, George T., et al. "Expression and Distribution of Phosphodiesterase Isoenzymes in the Human Male Urethra." Urology (2015). PMID25704994	Immunohistochemistry, Western Blot; Human
Nordgaard, Julie Carøe, et al. "Role of Ser102 and Ser104 as Regulators of cGMP Hydrolysis by PDE5A." PloS one 9.9 (2014): e107627. PMID25247292	Immunofluorescence, Western Blot; Human
Akkerman, Sven et al. “PDE5 Inhibition Improves Object Memory in Standard Housed Rats but Not in Rats Housed in an Enriched Environment: Implications for Memory Models?” Ed. Patrizia Campolongo. PLoS ONE 9.11 (2014): e111692. PMC4221101	Western Blot; Rat
Witting, Nanna, et al. "Effect of sildenafil on skeletal and cardiac muscle in Becker muscular dystrophy." Annals of neurology 76.4 (2014): 550-557. PMID25042931	Western Blot; Mouse
Carøe Nordgaard, Julie et al. “Role of Ser102 and Ser104 as Regulators of cGMP Hydrolysis by PDE5A.” Ed. David D. Roberts. PLoS ONE 9.9 (2014): e107627. PMC4172485	Western Blot; Human
Mietens, Andrea, et al. "Phosphodiesterase 5 (PDE5) inhibition, ANP and NO rapidly reduce epididymal duct contractions, but long-term PDE5 inhibition in vivo does not." Molecular and cellular endocrinology 349.2 (2012): 145-153. PMID21996373	Immunohistochemistry, Western Blot; Huma, Rat
Shukla, Praveen, Chengwen Sun, and Stephen T. O’Rourke. “Melatonin Inhibits Nitric Oxide Signaling by Increasing PDE5 Phosphorylation in Coronary Arteries.” American Journal of Physiology - Heart and Circulatory Physiology 303.12 (2012): H1418–H1425. PMC3532536	Western Blot; Pig
Ückert, Stefan, et al. "Expression and distribution of phosphodiesterase isoenzymes in the Human seminal vesicles." The journal of sexual medicine 8.11 (2011): 3058-3065. PMID21810184	Immunohistochemistry, Western Blot; Human
Luong, Christina, et al. "Antenatal sildenafil treatment attenuates pulmonary hypertension in experimental congenital diaphragmatic hernia." Circulation 123.19 (2011): 2120-2131. PMID21537000	Western Blot; Rat
Adderley, Shaquria P. et al. “Identification of Cytosolic Phosphodiesterases in the Erythrocyte: A Possible Role for PDE5.” Medical Science Monitor : International Medical Journal of Experimental and Clinical Research 17.5 (2011): CR241–CR247. PMC3366467	Western Blot; Human, Rabbit
Ückert, S, et al. "Expression And Distribution Of Key Enzymes Of The Cyclic GMP Signaling In The Human Clitoris: Relation To Phosphodiesterase Type 5 (PDE5)." International Journal Of Impotence Research 23.5 (2011): 206-212. PMID21697861	Immunohistochemistry; Human
Mokni, Walid et al. “Concerted Regulation of cGMP and cAMP Phosphodiesterases in Early Cardiac Hypertrophy Induced by Angiotensin II.” Ed. Karl-Wilhelm Koch. PLoS ONE 5.12 (2010): e14227. PMC2997062	Immunohistochemistry, Western Blot; Rat
Murthy, K S. “Contractile Agonists Attenuate cGMP Levels by Stimulating Phosphorylation of cGMP-Specific PDE5; an Effect Mediated by RhoA/PKC-Dependent Inhibition of Protein Phosphatase 1.” British Journal of Pharmacology 153.6 (2008): 1214–1224. PMC2275441	Immunoprecipitation, Western Blot; Rabbit
Sasseville, Maxime, et al. "Partie 5 3’5’-cyclic guanosine monophosphate-specific phosphodiesterases in the porcine cumulus-oocyte complex." Endocrinology. 2008 Nov;149(11):5568-76. doi: 10.1210/en.2008-0547. Epub 2008 Jul 31 PMID18669600	Immunodepletion, Western Blot; Pig
Albrecht, Knut, et al. "Immunohistochemical distribution of cyclic nucleotide phosphodiesterase (PDE) isoenzymes in the Human vagina: A potential forensic value?." Journal of forensic and legal medicine 14.5 (2007): 270-274. PMID17113813	Immunohistochemistry; Human
Ückert, Stefan, et al. "Immunohistochemical Description of Cyclic Nucleotide Phosphodiesterase (PDE) Isoenzymes in the Human Labia Minora." The Journal of Sexual Medicine 4.3 (2007): 602-608. PMID17498099	Immunofluorescence; Human
Werkström, Viktoria, et al. "Phosphodiesterase 5 In The Female Pig And Human Urethra: Morphological And Functional Aspects."BJU International 98.2 (2006): 414-423. PMID16626307	Immunohistochemistry; Human, Pig
Liu, Wu-Jiang, et al. "Effects of icariin on erectile function and expression of nitric oxide synthase isoforms in castrated rats." Asian journal of andrology 7.4 (2005): 381. PMID16281085	Western Blot; Rat
Wang, Lei, et al. "Activation of the PI3-K/Akt pathway mediates cGMP enhanced-neurogenesis in the adult progenitor cells derived from the subventricular zone." Journal of Cerebral Blood Flow & Metabolism 25.9 (2005): 1150-1158. PMID15815584	Immunohistochemistry, Western Blot; Rat
Ückert, Stefan, et al. "Cyclic adenosine monophosphate and cyclic guanosine monophosphate-phosphodiesterase isoenzymes in Human vagina: relation to nitric oxide synthase isoforms and vasoactive intestinal polypeptide-containing nerves." Urology 65.3 (2005): 604-610. PMID15780401	Immunohistochemistry; Human
Monfort, Pilar, María‐Dolores Muñoz, and Vicente Felipo. "Chronic hyperammonemia in vivo impairs long‐term potentiation in hippocampus by altering activation of cyclic GMP‐dependent‐protein kinase and of phosphodiesterase 5." Journal of neurochemistry 94.4 (2005): 934-942. PMID16092938	Western Blot; Rat

Publishing research using PD5A-101AP? Please let us know, we can cite the reference in this datasheet.

To view the Specification Sheet for PDE5A Antibody, please click the link below:

PDE5A Antibody | FabGennix

I am looking for an antibody for PDE5. I would like to use this antibody on rat lung tissue for immunohistochemistry staining, immunofluorescence staining and western blot. On the internet I can find some antibodies, but I’m not sure if they will work well enough on rat. Do you have an antibody which you can recommend for this?
1. Our PDE5A antibody (PD5A-101AP) will work in immunohistochemistry staining, immunofluorescence staining and western blot with rat tissues. If you visit the publications tab under the PD5A-101AP product page you can see a summary of several publications that used this antibody. PMID21996373, PMC2997062 and PMID15815584 are some papers that utilize both immunohistochemistry and Western Blot in rat samples. We also have a phospho specific PDE5A antibody (PPD5A-140AP) that has been validated in the same applications and tissues.

Customer ratings for PDE5A Antibody

Number of ratings: 2
Average rating: 3.5

IHC with paraffin-embedded sections on human tissue. 1:10 primary antibody dilution overnight.
from Joseph Alzar on 03/22/2024

Good
from Anonymous on 05/28/2016

IHC with paraffin-embedded sections on human tissue. 1:10 primary antibody dilution overnight.

All incoming ratings are posted by actual shop visitors / webshop customers and their content is checked by the operator by means of a visual inspection.

Accessories

Product

Note

Status

Price

PDE5A Antibody FITC

PD5A-FITC

$375.00 *

PDE5A Antibody BIOTIN

PD5A-BIOTIN

$375.00 *

PDE5A Blocking Peptide

P-PD5A

$155.00 *

HRP-Conjugated PDE5A Antibody

PD5A-HRP

$375.00 *

PDE5A Positive Control

PC-PD5A

$220.00 *

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We also recommend

PPD5A-140AP

Phospho-PDE5A Antibody

$375.00 *

Antibody against recombinant PDE5A

PDE5A Antibody

$285.00 *

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Phospho-PDE5A Antibody $375.00 *	PDE2A4 Antibody $285.00 *	PDE4D Antibody $320.00 *
PDE3A Antibody $320.00 *	PDE2A Antibody $320.00 *

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Browse these categories as well: cGMP, Oxidative Stress, Atherosclerosis, Hypoxia, Metabolism, Phosphodiesterases, Primaries

PDE6 Alpha Antibody

Product no.: PD6A-101AP

$320.00

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Description
Publications

General Information
Product	Pan PDE6 Alpha Antibody
Description	Affinity Purified Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha Antibody
Verified Applications	CM, ELISA, ICC, IF, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic cyclic peptide common to all PDE6 Alpha variants
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.50-0.75 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:250
DB	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:250
Immunofluorescence	1:250
Immunohistochemistry	1:250
Immunoprecipitation	1:250
Western Blot	1:500
Protein
Uniprot #	P51160
Molecular Function	Hydrolase, 3',5'-cyclic-GMP phosphodiesterase activity, cGMP binding
Subcellular Location	Cell membrane; Lipid-anchor; Cytoplasmic side
Structure	Composed of two alpha' subunits that are associated with 3 smaller proteins of 11, 13, and 15 kDa.
Alternative Nomenclature	5''-cyclic phosphodiesterase subunit alpha antibody CGPR A antibody GMP PDE alpha antibody PDE 6 alpha antibody PDE V B1 antibody PDEA antibody Phosphodiesterase 6 alpha antibody Retinal Rod Photoreceptor cGMP Rod cGMP specific 3' 5' cyclic phosphodiesterase alpha subunit antibody

Protein information supplied by UniProt

Accessories

Product

Note

Status

Price

PDE6 Alpha Antibody FITC

PD6A-FITC

$375.00 *

PDE6 Alpha Antibody BIOTIN

PD6A-BIOTIN

$375.00 *

PDE6 Alpha Blocking Peptide

P-PD6A

$155.00 *

PDE6 Alpha Positive Control

PC-PD6A

$220.00 *

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PDE6C-101AP

PDE6C / PDE6 Delta Antibody

$285.00 *

PD6-201AP

PDE6 Beta Antibody

$320.00 *

PD6-301AP

PDE6 Gamma Antibody

$320.00 *

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Browse these categories as well: cAMP, cGMP, Vision, Primaries, Phosphodiesterases, Vision & Olfaction

PDE6 Beta Antibody

Product no.: PD6-201AP

$320.00

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Description
Publications

General Information
Product	PDE6 Beta Antibody
Description	Affinity Purified Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta Antibody
Verified Applications	ELISA, CM, ICC, IF, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Bovine, Human, Mouse, Pig, Rat
Immunogen	Synthetic cyclic petide common to all PDE6 Beta variants
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.5 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:200
DB	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:200
Immunofluorescence	1:200
Immunohistochemistry	1:200
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	P35913
Overview	This protein participates in processes of transmission and amplification of the visual signal. Necessary for the formation of a functional phosphodiesterase holoenzyme.
Molecular Function	Hydrolase, 3', 5' -cyclic-AMP phosphodiesterase activity
Subcellular Location	Membrane; lipid anchor
Structure	Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.
Alternative Nomenclature	5''-cyclic phosphodiesterase subunit beta antibody Congenital stationary night blindness 3 autosomal dominant antibody CSNB 3 antibody CSNBAD2 antibody DPDE 4 antibody GMP-PDE beta antibody PDE 6 beta antibody PDE 6B antibody PDEB antibody Phosphodiesterase 6B antibody Rd 1 antibody Rod cGMP specific 3' 5' cyclic phosphodiesterase beta subunit antibody Rod cGMP-specific 3'' antibody RP40 antibody

Protein information supplied by UniProt

Accessories

Product

Note

Status

Price

PDE6 Beta Antibody FITC

PD6B-FITC

$375.00 *

PDE6 Beta Antibody BIOTIN

PD6B-BIOTIN

$375.00 *

PDE6 Beta Blocking Peptide

P-PD6B

$155.00 *

PDE6 Beta Positive Control

PC-PD6B

$220.00 *

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PD6-301AP

PDE6 Gamma Antibody

$320.00 *

PD6A-101AP

PDE6 Alpha Antibody

$320.00 *

PDE6C-101AP

PDE6C / PDE6 Delta Antibody

$285.00 *

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Browse these categories as well: cAMP, cGMP, Vision, Primaries, Phosphodiesterases, Vision & Olfaction

PDE6 Gamma Antibody

Product no.: PD6-301AP

$320.00

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Description
Publications

General Information
Product	PDE6 Gamma Antibody
Description	Affinity Purified Retinal cone Phosphodiesterase 6 gamma Antibody
Verified Applications	ELISA, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Bovine, Human, Mouse, Rat
Immunogen	Synthetic cyclic peptide common to all PDE6 gamma variants.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.68 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DOT Blot	1:10,000
ELISA	1:10,000
Immunohistochemistry	1:50-1:200
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Overview	Visual perception
Molecular Function	3',5'-cyclic-nucleotide phosphodiesterase activity, cGMP binding
Alternative Nomenclature	5''-cyclic phosphodiesterase subunit gamma antibody GMP-PDE gamma antibody PDE 6 antibody PDEG antibody Phosphodiesterase 6G cGMP Specific antibody Retinal Rod Photoreceptor cGMP Phosphodiesterase gamma antibody RP57 antibody

Protein information supplied by UniProt

Accessories

Product

Note

Status

Price

PDE6 Gamma Antibody FITC

PD6G-FITC

$375.00 *

PDE6 Gamma Antibody BIOTIN

PD6G-BIOTIN

$375.00 *

PDE6 Gamma Blocking Peptide

P-PD6G

$155.00 *

PDE6 Gamma Positive Control

PC-PD6G

$220.00 *

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PDE6C-101AP

PDE6C / PDE6 Delta Antibody

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PD6A-101AP

PDE6 Alpha Antibody

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PD6-201AP

PDE6 Beta Antibody

$320.00 *

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PDE9A Antibody

Product no.: PD9A-101AP

$320.00

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General Information
Product	PDE9A Antibody
Description	Affinity Purified High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A isoform a Antibody
Verified Applications	CM, ELISA, ICC/IF, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic cyclic peptide common to all PDE9A variants.
Specificity	This antibody does not cross-react with PDE9B protein or any other PDE family members.
Accession #	NCBI: NP_002597.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.55 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:100
DB	1:100
ELISA	1:100
Immunocytochemistry	1:100
Immunofluorescence	1:100
Immunohistochemistry	1:100
Immunoprecipitation	1:250
Western Blot	1:500
Protein
Uniprot #	O76083
Overview	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction.
Molecular Function	Hydrolase, 3', 5' -cyclic-GMP phosphodiesterase activity
Subcellular Location	Isoform PDE9A1: Cell projection, Cytoplasm, Golgi Apparatus, Endoplasmic reticulum, Isoform PDE9A2: Cell projection, Cytoplasm, Isoform PDE9A3: Cytoplasm, Endoplasmic Reticulum, Isoform PDE9A17: Cytoplasm, Endoplasmic reticulum
Expression	Expressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood. Highest levels in brain, heart, kidney, spleen, prostate and colon. Isoform PDE9A12 is found in prostate.
Alternative Nomenclature	FLJ90181 antibody HSPDE9A2 antibody PDE 9A antibody Pde9a antibody Phosphodiesterase 9A antibody phosphodiesterase PDE9A21 antibody

Protein information supplied by UniProt

Fabgennix PDE9A Antibody has been used in 5 Publications

Dhingra, Anuradha, et al. "PDE9A is expressed in the inner retina and contributes to the normal shape of the photopic ERG waveform." Frontiers in molecular neuroscience 7 (2014). PMID25018695

Immunofluorescence, Western Blot; Mouse

Nagasaki, Shuji, et al. "Phosphodiesterase Type 9 (PDE9) In The Human Lower Urinary Tract: An Immunohistochemical Study."BJU International 109.6 (2012): 934-940. PMID21736695

Immunohistochemistry, Western Blot; Human

Kruse, Lars S., et al. "PDE9A, PDE10A, and PDE11A expression in Rat trigeminovascular pain signalling system." Brain research 1281 (2009): 25-34. PMID19445908

Immunohistochemistry, Immunodepletion, Western Blot; Rat

Almeida, Camila Bononi, et al. "High Expression Of The Cgmp-Specific Phosphodiesterase, PDE9A, In Sickle Cell Disease (SCD) And The Effects Of Its Inhibition In Erythroid Cells And SCD Neutrophils." British Journal Of Haematology 142.5 (2008): 836-844. PMID18564357

Western Blot; Human

Baxendale, Rhona W., and Lynn R. Fraser. "Mammalian sperm phosphodiesterases and their involvement in receptor‐mediated cell signaling important for capacitation." Molecular reproduction and development 71.4 (2005): 495-508. PMID15856425

Immunofluorescence, Western Blot; Mouse

Publishing research using PD9A-101AP? Please let us know, we can cite the reference in this datasheet.

To view the Specification Sheet for PDE9A Antibody, please click the link below:

PDE9A Antibody | FabGennix

Accessories

Product

Note

Status

Price

PDE9A Antibody FITC

PD9A-FITC

$375.00 *

PDE9A Antibody BIOTIN

PD9A-BIOTIN

$375.00 *

PDE9A Blocking Peptide

P-PD9A

$155.00 *

PDE9A Positive Control

PC-PD9A

$220.00 *

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Phospho-PDE3A Antibody

Product no.: PPD3A-140AP

$375.00

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Description
Specification Sheet

General Information
Product	Phospho-PDE3A Antibody
Description	Affinity Purified Phosphorylated cGMP-inhibited 3',5'-cyclic phosphodiesterase A Antibody
Verified Applications	ELISA, IP, WB
Host	Rabbit
Species Cross Reactivity	Chicken, Mouse, Rat Predicted: Human
Immunogen	Synthetic peptide taken within amino acid region 200-300 on mouse PDE3A protein. Phosphorylated Serine 290 & 292 residues.
Specificity	This antibody will detect PDE3A protein that has been phosphorylated at its putative PKB/Akt site.
Accession #	NCBI: NP_061249.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	1.0-1.25 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Modification	Phosphorylated Serine 290 and Serine 292.
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	Q9Z0X4
Overview	Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Involved in oocyte maturation.
Molecular Function	Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding
Subcellular Location	Membrane; Multipass membrane protein
Alternative Nomenclature	5''-cyclic phosphodiesterase A antibody cAMP phosphodiesterase myocardial cGMP inhibited antibody CGI PDE antibody CGI PDE A antibody cGMP inhibited 3' 5' cyclic phosphodiesterase A antibody Cyclic GMP-inhibited phosphodiesterase A antibody Human cGMP inhibited cAMP phosphodiesterase mRNA antibody PDE 3A antibody Phosphodiesterase 3A antibody Phosphodiesterase 3A cGMP inhibited antibody

Protein information supplied by UniProt

Accessories

Product

Note

Status

Price

Phospho-PDE3A Antibody FITC

PPD3A-FITC

$425.00 *

Phospho-PDE3A Antibody BIOTIN

PPD3A-BIOTIN

$425.00 *

Phospho-PDE3A Blocking Peptide

P-PPD3A

$195.00 *

Phospho-PDE3A Positive Control

PC-PPD3A

$220.00 *

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Rabbit Polyclonal PDE3A PDE3A Antibody $320.00 *	Goat Polyclonal PDE3A PDE3A Antibody $320.00 *	Rabbit Polyclonal PDE3B PDE3B Antibody $320.00 *
Goat Polyclonal PDE3B PDE3B Antibody $320.00 *

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Phospho-PDE5A Antibody

Product no.: PPD5A-140AP

$375.00

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General Information
Product	Phospho-PDE5A Antibody
Description	Affinity Purified Phosphorylated Phosphodiesterase 5A Antibody
Verified Applications	ELISA, ICC, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Bovine, Human, Mouse, Pig, Rat, Sheep
Immunogen	Synthetic peptide common to all PDE5A variants taken within amino acid region 120-170 on human PDE5A protein. Phosphoryalted
Specificity	This antibody will detect only PDE5A protein that has been phosphorylated at the PKG site. Will not detect unphosphorylated PDE5A, or other PDE family members.
Accession #	GenBank: EAX05277.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	1.0-1.25 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:100
DB	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:100
Immunofluorescence	1:100
Immunohistochemistry	1:100
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	UPI0000EE2B22
Overview	Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.
Molecular Function	Hydrolase, 3',5'-cyclic-GMP phosphodiesterase activity, cGMP binding
Subcellular Location	cytosol
Expression	Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
Alternative Nomenclature	5''-cyclic phosphodiesterase antibody CGB PDE antibody cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody cGMP binding/cGMP specific phosphodiesterase antibody cGMP specific phosphodiesterase type 5A antibody CN5A antibody CN5N antibody PDE 5 antibody PDE 5A antibody PDE5A1 antibody Phosphodiesterase 5A antibody Phosphodiesterase 5A cGMP specific antibody Phosphodiesterase isozyme 5 antibody

Protein information supplied by UniProt

Fabgennix Phospho-PDE5A Antibody has been used in 14 Publications
MHerrera, Emilio A., et al. "The role of nitric oxide signaling in pulmonary circulation of highand low-altitude newborn sheep under basal and acute hypoxic conditions." Nitric Oxide, 89, 71-80. PMID: 31063821	Western Blot; *Sheep*
Musicki, Biljana, et al. "Testosterone replacement in transgenic sickle cell mice controls priapic activity and upregulates PDE5 expression and eNOS activity in the penis." Andrology 6.1 (2018): 184-191. PMID29145710	Western Blot; *Mouse*
Nino, Paula Katherina Bautista, et al. "Phosphodiesterase 1 regulation is a key mechanism in vascular aging." Clinical Science (2015): CS20140753. PMID26464516	Western Blot; *Mouse*
Nino, Paula Katherina Bautista, et al. "Phosphodiesterase 1 regulation is a key mechanism in vascular aging." Clinical Science (2015): CS20140753. PMID26464516	Western Blot; *Mouse*
Nino, Paula Katherina Bautista, et al. "Phosphodiesterase 1 regulation is a key mechanism in vascular aging." Clinical Science (2015): CS20140753. PMID26464516	Western Blot; *Mouse*
Rajagopal, Senthilkumar, et al. "Cytokine-Induced S-Nitrosylation of Soluble Guanylyl Cyclase and Expression of Phosphodiesterase 1A Contribute to Dysfunction of Longitudinal Smooth Muscle Relaxation." Journal of Pharmacology and Experimental Therapeutics 352.3 (2015): 509-518.PMID25550199	Western Blot; *Mouse*

Click Here for the complete list of all FabGennix Phospho-PDE5A Antibody publications

Publishing research using PPD5A-140AP? Please let us know, we can cite the reference in this datasheet.

To view the Specification Sheet for Phospho-PDE5A Antibody, please click the link below:

Phospho-PDE5A Antibody | FabGennix

Which phosphorylation site of PDE5A does this Phospho-PDE5A Antibody (PPD5A-140AP) recognize?
1. This antibody detects only PDE5A protein phosphorylated at Serine 148. This Antibody will detect phosphorylation at the PKG site.