General Information
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Product
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SQSTM1 / p62 Antibody
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Verified Applications
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ELISA, IHC, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 400-440 on human Sequestosome-1 protein.
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.5 µg/µl in antibody stabilization buffer
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Immunoglobulin
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IgG
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Form
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Affinity Purified
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Purification Method
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Immobilized antigen affinity chromatography
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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ELISA
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1:10,000
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Immunohistochemistry |
1:50-1:200 |
Immunoprecipitation
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1:150
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Western Blot
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1:500
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Protein
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Uniprot #
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Q13501
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Overview
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Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD. May be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels.
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Molecular Function
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identical protein binding, protein kinase binding, protein kinase C binding, protein serine/threonine kinase activity, receptor tyrosine kinase binding, SH2 domain binding, ubiquitin binding, ubiquitin protein ligase binding, zinc ion binding
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Subcellular Location
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Cytoplasm
Late endosome
Lysosome
Cytoplasmic vesicle › autophagosome
Nucleus
Endoplasmic reticulum
Cytoplasm › P-body
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Expression
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Ubiquitously expressed.
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Structure
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Homooligomer or heterooligomer; may form homotypic arrays. Dimerization interferes with ubiquitin binding.
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Alternative Nomenclature
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A170
EBI 3 associated protein of 60 kDa
EBIAP
FTDALS3
ORCA
OSF-6
Osi
OSIL
Oxidative stress induced like
p60
p62
p62B
Paget disease of bone 3
PDB3
Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa
PKC-zeta-interacting protein
Protein kinase C-zeta-interacting protein
Sequestosome 1
SQSTM 1
STAP
STONE14
Ubiquitin-binding protein p62
ZIP
ZIP3
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