General Information |
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Product |
PDE4D5 Positive Control |
Description |
Western bot positi cAMP-specific phosphodiesterase PDE4D5 |
Verified Applications |
Western blot |
Accession # |
GenBank: AAC00069.1 |
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Quantity |
5 applications |
Volume |
Inquire |
Form |
Wester blot positive control |
Concentration | The concentration of the sample is empirically set so that 20 µl per lane in Western and blotted with corresponding affinity purified antibody will give a clean band with a high signal to noise ratio on the gel. |
Storage |
-20⁰C for long term storage |
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Uniprot # |
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Overview |
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. |
Molecular Function |
Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding |
Subcellular Location |
Apical cell membrane, Cytoplasm, Membrane |
Expression |
Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle. |
Structure |
Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphotase 1 (PP1) (By similarity). Isoform 5, isoform N3 and isoform 12 bind GNB2L1 via their unique N-terminus. Binds ARRB2. Interacts (via N-terminal region) with SHANK2 (via proline-rich region); the interaction is increased in a PKA-dependent manner. |
Alternative Nomenclature |
cAMP-specific 3'5'-cyclic phosphodiesterase 4D antibody DPDE3 antibody dunce-like phosphodiesterase E3 antibody hPDE4D5 antibody PDE4D antibody PDE4DN2 antibody Phosphodiesterase 4D antibody Phosphodiesterase 4D cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E3) antibody Phosphodiesterase 4D cAMP-specific antibody STRK1 antibody |
Protein information supplied by UniProt
Fabgennix PDE4D5 Antibody has been used in 6 Publications |
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Wang, Zhen-Zhen, et al. "Phosphodiesterase-4D Knock-down in the Prefrontal Cortex Alleviates Chronic Unpredictable Stress-Induced Depressive-Like Behaviors and Memory Deficits in Mice." Scientific Reports 5 (2015). PMID26161529
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Western Blot; Mouse |
Mahavadi, Sunila, et al. "Cytokine-induced iNOS and ERK1/2 inhibit adenylyl cyclase type 5/6 activity and stimulate phosphodiesterase 4D5 activity in intestinal longitudinal smooth muscle." American Journal of Physiology-Cell Physiology 307.4 (2014): C402-C411. PMID24944202
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Western Blot; Mouse |
Li, Yun-Feng et al. “Phosphodiesterase-4D Knockout and RNAi-Mediated Knockdown Enhance Memory and Increase Hippocampal Neurogenesis via Increased cAMP Signaling.” The Journal of neuroscience : the official journal of the Society for Neuroscience 31.1 (2011): 172–183. PMC3079568
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Immunohistochemistry, Western Blot; Mouse |
Nino, Gustavo et al. “Mechanism Regulating Proasthmatic Effects of Prolonged Homologous Β2-Adrenergic Receptor Desensitization in Airway Smooth Muscle.” American Journal of Physiology - Lung Cellular and Molecular Physiology 297.4 (2009): L746–L757. PMC2770790
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Western Blot; Rabbit |
Murthy, Karnam S., and Wimolpak Sriwai. "Stimulatory phosphorylation of cAMP-specific PDE4D5 by contractile agonists is mediated by PKC-dependent inactivation of protein phosphatase 2A." American Journal of Physiology-Gastrointestinal and Liver Physiology 294.1 (2008): G327-G335. PMID18006600
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Immunoprecipitation, Western Blot; Human |
Hajjhussein, Hassan et al. “Changes in NMDA Receptor-Induced Cyclic Nucleotide Synthesis Regulate the Age-Dependent Increase in PDE4A Expression in Primary Cortical Cultures.” Brain research 1149 (2007): 58–68. PMC2720317
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Immunoprecipitation, Western Blot; Human |
Publishing research using PD4D5-451AP? Please let us know, we can cite the reference in this datasheet.
Product | Note | Status | Price | |
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PD4D5-451AP | |||
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PD4D5-FITC | |||
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P-PD4D5 | |||
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PD4D5-BIOTIN | |||
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