Custom antibody production frequently asked questions (FAQs)

 

 

I am interested in protein antigen expression and polyclonal antibody production (rabbit) as well as peptide prediction, synthesis, conjugation, and immunization. Can you make an antibody that works in IHC or FACs? What about western blot?

 

Yes, Fabgennix will provide all the services listed here. We can clone, express and purify the target protein with a tag in prokaryotic as well as mammalian cells. We can also perform complete proteomics analysis to select the antigenic regions that are exposed on the surface when the protein is presented in its native conformation. The antibodies generated against epitopes that are not only antigenic, but also lie on the surface of the proteins tend to work best for cell labeling or IHC applications. 

 

In which way do you control the quality of peptides and how do you predict the appropriate peptide for antibody production?  

 

We use solid phase Fmoc chemistry for peptide synthesis. Once the crude peptide is analyzed for mass spec for complete synthesis we can further purify the peptide on preparative HPLC using RP columns. The purity of the peptide is determined by HPLC. We can get up to 98-99% pure peptide.. 

 

What about the application of this antibody? I would like to use it in Western Blot and FACS. 

 

We will perform the proteomics analysis for selection of antigenic peptides that are exposed. This will have greater chances of recognizing the target protein in its native conformation. For IHC applications we will help you in antigen retrieval and optimization of protocol.  

 

What will be if this antibody will not work in our lab?

 

If you can provide recombinant protein for over expressing cells extracts we will guarantee that these antibodies will work in WB.

 

 

Can you provide a quotation for my custom antibody project?

 

We can get you an estimate for your custom antibody project. Click here to use our custom antibody form so we can provide you an accurate quote.