General Information
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Product
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U2AF59 Antibody FITC
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Description
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FITC-Conjugated Splicing factor U2AF 59 kDa subunit Antibody
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Verified Applications
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ELISA, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Saccharomyces cerevisiae
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Immunogen
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Synthetic peptide taken within amino acid region 480-517 on yeast U2AF59 protein.
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.75-1.13 µg/µl in antibody stabilization buffer
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Form
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FITC-Conjugated
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:10,000
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ELISA
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1:10,000
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Immunoprecipitation
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1:200
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Western Blot
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1:500-1:750
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Protein
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Uniprot #
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P36629
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Overview
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Necessary for the splicing of pre-mRNA. The SF1-U2AF59-U2AF23 complex has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.
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Molecular Function
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mRNA binding, snoRNA binding, nucleotide binding
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Subcellular Location
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Nucleus
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Structure
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Forms a heterodimer with the U2AF small subunit. Can also form a homodimer. U2AF large subunit (U2AF59), U2AF small subunit (U2AF23) and SF1 (bpb1) interact to form a complex required for complex A formation. Interacts with wat1/pop3.
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Alternative Nomenclature
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S. pombe splicing factor U2 59 kDa subunit
Schzosacharomyces pombe splicing factor U2 auxiliary factor
U2 auxiliary factor 59 kDa subunit
U2 snRNP auxiliary factor large subunit
mis11
prp2
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