General Information
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Product
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B-raf Antibody
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Description
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Affinity Purified Serine/threonine-protein kinase B-raf Antibody
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Verified Applications
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ELISA, IHC, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Monkey, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 700-750 on human serine/threonine-protein kinase B-raf protein.
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Accession #
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NCBI: NP_004324.2
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.5 µg/µl in antibody stabilization buffer
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Immunoglobulin
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IgG
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Form
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Affinity Purified
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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ELISA
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1:10,000
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Immunohistochemistry |
1:50-1:100 |
Immunoprecipitation
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1:200
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Western Blot
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1:500. Molecular Weight: 84.4
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Protein
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Uniprot #
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P15056
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Overview
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Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
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Molecular Function
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Kinase, Serine/threonine-protein kinase, Transferase
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Subcellular Location
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Nucleus, Cytoplasm, Cell membrane
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Expression
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Brain and testis.
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Structure
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Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-753. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1. Interacts with DGKH. Interacts with PRMT5. Interacts with KSR2.
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Alternative Nomenclature
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94 kDa B raf protein antibody
B raf 1 antibody
B Raf proto oncogene serine threonine protein kinase antibody
B RAF1 antibody
B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody
BRAF 1 antibody
cRmil antibody
Murine sarcoma viral v raf oncogene homolog B1 antibody
NS7 antibody
Oncogen BRAF antibody
p94 antibody
Proto-oncogene B-Raf antibody
Proto-oncogene c-Rmil antibody
RAFB1 antibody
RMIL antibody
Serine/threonine-protein kinase B-raf antibody
v-Raf murine sarcoma viral oncogene homolog B1 antibody
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