Phospho-MLKL Antibody

General Information

Product

Phospho-MLKL Antibody

Description

Affinity Purified Phosphorylated Mixed lineage kinase domain-like protein MLKL Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 350-400 on human Mixed lineage kinase domain-like protein MLKL

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Modification

Phosphorylated

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q8NB16

Overview

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Does not have protein kinase activity (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671).

Molecular Function

ATP binding

identical protein binding

JUN kinase kinase kinase activity

protein-containing complex binding

protein kinase activity

protein kinase binding

Subcellular Location

Nucleus, Plasma membrane, cytoplasm

Structure

Homooligomer (PubMed:29883610). Homotrimer; forms homotrimers on necroptosis induction (PubMed:24316671). Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3 (PubMed:22265414).

Alternative Nomenclature

9130019I15Rik antibody

FLJ34389 antibody

hMLKL antibody

Mixed lineage kinase domain like antibody

Mixed lineage kinase domain like pseudokinase antibody

Mixed lineage kinase domain-like protein antibody

Mlkl antibody

MLKL_HUMAN antibody