General Information

Product

Smad4 Antibody

Description

Affinity Purified Mothers against decapentaplegic homolog 4 Antibody Mid-Region Epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Fruit fly, Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-450 on human SMAD4 protein.

Accession #

NCBI:  NP_005350.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region Epitope

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13485

Overview

In muscle physiology, plays a central role in balance between atrophy and hypertrophy. May act as a tumor suppressor.

Molecular Function

Chromatin binding, core promoter proximal region sequence-specific DNA binding, identical protein binding I-SMAD binding, metal ion binding

Subcellular Location

Cytoplasm, nucleus (Note: Cytoplasmic in the absence of ligand. Migrates to nucleus when complexed with R-SMAD. PDPK1 prevents its nuclea translocation in response to TGF-beta.

Structure

Found in a complex with SMAD1 and YY1 (By similarity). Interacts with CITED2 (By similarity). Monomer; in the absence of TGF-beta activation. Heterodimer; on TGF-beta activation. Composed of two molecules of a C-terminally phosphorylated R-SMAD molecule, SMAD2 or SMAD3, and one molecule of SMAD4 to form the transcriptional active SMAD2/SMAD3-SMAD4 complex. Found in a ternary complex composed of SMAD4, STK11/LKB1 and STK11IP. Interacts with ATF2, COPS5, DACH1, MSG1, SKI, STK11/LKB1, STK11IP and TRIM33. Interacts with ZNF423; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with ZNF521; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with USP9X. Interacts (via the MH1 and MH2 domains) with RBPMS. Interacts with WWTR1 (via coiled-coil domain). Component of the multimeric complex SMAD3/SMAD4/JUN/FOS which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Interacts with CITED1. Interacts with PDPK1 (via PH domain) (By similarity). Interacts with VPS39; this interaction affects heterodimer formation with SMAD3, but not with SMAD2, and leads to inhibition of SMAD3-dependent transcription activation. Interactions with VPS39 and SMAD2 may be mutually exclusive.

Alternative Nomenclature

(Small) Mothers Against Decapentaplegic antibody Deleted in Pancreatic Carcinoma 4 antibody
Deleted in Pancreatic Carcinoma antibody DPC4 antibody
JIP antibody
MAD mothers against decapentaplegic Drosophila homolog 4 antibody

MADH4 antibody
Med antibody
Medea antibody

Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody

MYHRS antibody
SMA- and MAD-related protein 4 antibody
Smad 4 antibody
SMAD family member 4 antibody
SMAD mothers against DPP homolog 4 antibody

General Information

Product

Smad4 Antibody

Description

Affinity Purified Mothers against decapentaplegic homolog 4 Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 502-552 on human SMAD4 protein.

Accession #

NCBI:  NP_005350.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13485

Overview

In muscle physiology, plays a central role in balance between atrophy and hypertrophy. May act as a tumor suppressor.

Molecular Function

Chromatin binding, core promoter proximal region sequence-specific DNA binding, identical protein binding I-SMAD binding, metal ion binding

Subcellular Location

Cytoplasm, nucleus (Note: Cytoplasmic in the absence of ligand. Migrates to nucleus when complexed with R-SMAD. PDPK1 prevents its nuclea translocation in response to TGF-beta.

Structure

Found in a complex with SMAD1 and YY1 (By similarity). Interacts with CITED2 (By similarity). Monomer; in the absence of TGF-beta activation. Heterodimer; on TGF-beta activation. Composed of two molecules of a C-terminally phosphorylated R-SMAD molecule, SMAD2 or SMAD3, and one molecule of SMAD4 to form the transcriptional active SMAD2/SMAD3-SMAD4 complex. Found in a ternary complex composed of SMAD4, STK11/LKB1 and STK11IP. Interacts with ATF2, COPS5, DACH1, MSG1, SKI, STK11/LKB1, STK11IP and TRIM33. Interacts with ZNF423; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with ZNF521; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with USP9X. Interacts (via the MH1 and MH2 domains) with RBPMS. Interacts with WWTR1 (via coiled-coil domain). Component of the multimeric complex SMAD3/SMAD4/JUN/FOS which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Interacts with CITED1. Interacts with PDPK1 (via PH domain) (By similarity). Interacts with VPS39; this interaction affects heterodimer formation with SMAD3, but not with SMAD2, and leads to inhibition of SMAD3-dependent transcription activation. Interactions with VPS39 and SMAD2 may be mutually exclusive.

Alternative Nomenclature

(Small) Mothers Against Decapentaplegic antibody Deleted in Pancreatic Carcinoma 4 antibody
Deleted in Pancreatic Carcinoma antibody DPC4 antibody
JIP antibody
MAD mothers against decapentaplegic Drosophila homolog 4 antibody

MADH4 antibody
Med antibody
Medea antibody

Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody

MYHRS antibody
SMA- and MAD-related protein 4 antibody
Smad 4 antibody
SMAD family member 4 antibody
SMAD mothers against DPP homolog 4 antibody

General Information

Product

Smad4 Antibody

Description

Affinity Purified Mothers against decapentaplegic homolog 4 Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 131-181 on human SMAD4 protein.

Accession #

NCBI:  NP_005350.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13485

Overview

In muscle physiology, plays a central role in balance between atrophy and hypertrophy. May act as a tumor suppressor.

Molecular Function

Chromatin binding, core promoter proximal region sequence-specific DNA binding, identical protein binding I-SMAD binding, metal ion binding

Subcellular Location

Cytoplasm, nucleus (Note: Cytoplasmic in the absence of ligand. Migrates to nucleus when complexed with R-SMAD. PDPK1 prevents its nuclea translocation in response to TGF-beta.

Structure

Found in a complex with SMAD1 and YY1. Interacts with CITED2. Monomer; in the absence of TGF-beta activation. Heterodimer; on TGF-beta activation. Composed of two molecules of a C-terminally phosphorylated R-SMAD molecule, SMAD2 or SMAD3, and one molecule of SMAD4 to form the transcriptional active SMAD2/SMAD3-SMAD4 complex. Found in a ternary complex composed of SMAD4, STK11/LKB1 and STK11IP. Interacts with ATF2, COPS5, DACH1, MSG1, SKI, STK11/LKB1, STK11IP and TRIM33. Interacts with ZNF423; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with ZNF521; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with USP9X. Interacts (via the MH1 and MH2 domains) with RBPMS. Interacts with WWTR1 (via coiled-coil domain). Component of the multimeric complex SMAD3/SMAD4/JUN/FOS which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Interacts with CITED1. Interacts with PDPK1 (via PH domain) (By similarity). Interacts with VPS39; this interaction affects heterodimer formation with SMAD3, but not with SMAD2, and leads to inhibition of SMAD3-dependent transcription activation. Interactions with VPS39 and SMAD2 may be mutually exclusive.

Alternative Nomenclature

(Small) Mothers Against Decapentaplegic antibody Deleted in Pancreatic Carcinoma 4 antibody
Deleted in Pancreatic Carcinoma antibody DPC4 antibody
JIP antibody
MAD mothers against decapentaplegic Drosophila homolog 4 antibody

MADH4 antibody
Med antibody
Medea antibody

Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody

MYHRS antibody
SMA- and MAD-related protein 4 antibody
Smad 4 antibody
SMAD family member 4 antibody
SMAD mothers against DPP homolog 4 antibody

General Information

Product

Baboon Antibody

Description

Affinity Purified Baboon Antibody N-epitope

Verified Applications

ELISA, ICC/IF, IP, WB

Species Cross Reactivity

D. melanogaster

Immunogen

N-epitope Synthetic peptide corresponding to amino acid region 200-221 of Drosophila melanogaster protein. The peptide was covalently modified for conjugation.

Synthetic peptide:

CNKNENMKRIFEDYVPHRLTS

Accession #

GenBank:  U04692

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500. Predicted molecular weight: 73 kDa

Protein

Uniprot #

Q23975

Overview

Invovled in activin binding, GPCR kinase activity, serine threonine kinase activity and transforming growth factor beta receptor activity.

Molecular Function

Serine/threonine protein kinase; transferase; ATP and Nucleotide binding

Subcellular Location

Activin receptor complex; Membrane

Structure

Belongs to protein kinase superfamily and contains protein kinase domain.

Alternative Nomenclature

Activin A receptor 45A antibody

ATR1 antibody

Atr45A antibody

Bab antibody

Babo antibody

G protein coupled receptor kinase 3 antibody

Gprk3 antibody

STKE antibody

General Information

Product

Baboon Antibody

Description

Affinity Purified Baboon Antibody C-epitope

Verified Applications

ELISA, ICC/IF, IHC, IP, WB

Immunogen

C-epitope Synthetic peptide corresponding to amino acids 552-572 of Drosophila melanogaster Baboon protein. Peptide was covalently modified.

Sequence:

QPDPSIEEMKKVVCIEKCRPN

Accession #

GenBank:  U04692

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.66 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q23975

Overview

Invovled in activin binding, GPCR kinase activity, serine threonine kinase activity and transforming growth factor beta receptor activity.

Molecular Function

Serine/threonine protein kinase; transferase; ATP and Nucleotide binding

Subcellular Location

Activin receptor complex; Membrane

Structure

Belongs to protein kinase superfamily and contains protein kinase domain.

Alternative Nomenclature

Activin A receptor 45A antibody

ATR1 antibody

Atr45A antibody

Bab antibody

Babo antibody

G protein coupled receptor kinase 3 antibody

Gprk3 antibody

STKE antibody

General Information

Product

Wishful Thinking (WIT) Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 70-120 on WIT Wishful Thinking Drosophila melanogaster protein.

Accession #

NCBI:  AF237561.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9NGX8

Overview

ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.

Molecular Function

ATP binding, Protein kinase activity, transforming growth factor beta receptor activity, type II

Subcellular Location

Early endosome, plasma membrane, integral component of membrane

Structure

Contains protein kinase domain

Alternative Nomenclature

Wit

Wishfulthinking

Wishful thinking WIT

CG10776

STK-D

General Information

Product

Wishful Thinking (WIT) Antibody

Description

Affinity Purified Wishful thinking (WIT) mRNA Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide taken within amino acid region 700-750 on WIT Wishful Thinking Drosophila melanogaster protein.

Accession #

NCBI:  AF237561.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9NGX8

Overview

ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.

Molecular Function

ATP binding, Protein kinase activity, transforming growth factor beta receptor activity, type II

Subcellular Location

Early endosome, plasma membrane, integral component of membrane

Structure

Contains protein kinase domain

Alternative Nomenclature

Wit

Wishfulthinking

Wishful thinking WIT

CG10776

STK-D

General Information

Product

Phantom Antibody

Description

Affinity Purified Phantom isoform A Antibody N-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

N-epitope synthetic peptide corresponding to unique amino acid sequences on D. melanogaster Phantom protein.

Accession #

NCBI: NP_573319.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9VWR5

Overview

Plays a role in the metabolism of insect hormones through ecdysteroid C25-hydroxylase activity.  May also funciton in the breakdown of synthetic insecticides.

Molecular Function

Monooxygenase, Oxireductase

Subcellular Location

Endoplasmic reticulum membrane, Peripheral membrane protein, Microsome membrane

Expression

First detected at early blastoderm stage 4, and stripes of expression remain through step 10.  Expression is not detectable during germ band retraction and some expression resumes during stae 15-17.  Low levels of expression are detected in larval brain and fat body.

Alternative Nomenclature

Cytochrome P450 306a1 antibody

Dmphm antibody

Exo antibody

EXO1 antibody

Exocephalon antibody

Protein phantom antibody

General Information

Product

Phantom Antibody

Description

Affinity Purified Phantom isoform A Antibody Mid-Region

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Mid region epitope synthetic peptide corresponding to unique amino acid sequences on D. melanogaster Phantom protein.

Accession #

NCBI: NP_573319.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9VWR5

Overview

Plays a role in the metabolism of insect hormones through ecdysteroid C25-hydroxylase activity.  May also funciton in the breakdown of synthetic insecticides.

Molecular Function

Monooxygenase, Oxireductase

Subcellular Location

Endoplasmic reticulum membrane, Peripheral membrane protein, Microsome membrane

Expression

First detected at early blastoderm stage 4, and stripes of expression remain through step 10.  Expression is not detectable during germ band retraction and some expression resumes during stae 15-17.  Low levels of expression are detected in larval brain a

Alternative Nomenclature

Cytochrome P450 306a1 antibody

Dmphm antibody

Exo antibody

EXO1 antibody

Exocephalon antibody

Protein phantom antibody

General Information

Product

Phantom Antibody Affinity Purified

Description

Affinity Purified Phantom isoform A Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

C-epitope synthetic peptide corresponding to unique amino acid sequences on D. melanogaster Phantom protein.

Accession #

NCBI: NP_573319.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9VWR5

Overview

Plays a role in the metabolism of insect hormones through ecdysteroid C25-hydroxylase activity.  May also funciton in the breakdown of synthetic insecticides.

Molecular Function

Monooxygenase, Oxireductase

Subcellular Location

Endoplasmic reticulum membrane, Peripheral membrane protein, Microsome membrane

Expression

First detected at early blastoderm stage 4, and stripes of expression remain through step 10.  Expression is not detectable during germ band retraction and some expression resumes during stae 15-17.  Low levels of expression are detected in larval brain a

Alternative Nomenclature

Cytochrome P450 306a1 antibody

Dmphm antibody

Exo antibody

EXO1 antibody

Exocephalon antibody

Protein phantom antibody