General Information |
|
Product |
PDE10A Antibody FITC-Conjugated |
Description |
FITC-Conjugated Phosphodiesterase 10A |
Verified Applications |
CM, ELISA, ICC, IF, IHC, IP, WB |
Host |
Rabbit |
Species Cross Reactivity |
Human, Monkey, Mouse, Rat |
Immunogen |
Synthetic peptide (Human) taken against a common sequence near the 250-325 amino acids downstream of N terminal region of PDE10A. Synthetic MAP. |
Specificity | This antibody has no cross-reactivity against any other PDE family members tested so far. This antibody will detect all PDE10A variants and subtypes. |
Accession # |
GenBank: AAD32596.1 |
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|
Quantity |
100 µg |
Volume |
200 µl |
Concentration |
0.7 µg/µl in antibody stabilization buffer |
Form |
FITC-Conjugated |
Clonality |
Polyclonal |
Storage |
-20⁰C for long term storage |
|
|
Confocal Microscopy |
1:100-1:150 |
DB |
1:10,000 |
ELISA |
1:10,000 |
Immunocytochemistry |
1:100-1:150 |
Immunofluorescence |
1:100-1:150 |
Immunohistochemistry |
1:100-1:150 |
Immunoprecipitation |
1:200 |
Western Blot |
1:500 |
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|
Uniprot # |
|
Overview |
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. |
Molecular Function |
Hydrolase, metal-ion binding, cGMP binding |
Subcellular Location |
Cytoplasm |
Expression |
Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum. |
Structure |
Homodimer |
Alternative Nomenclature |
5''-cyclic phosphodiesterase 10A antibody cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A antibody cAMP and cAMP-inhibited cGMP 3'' antibody FLJ11894 antibody HSPDE10A antibody Phosphodiesterase 10A antibody |
Protein information supplied by UniProt
Fabgennix PDE10A Antibody has been used in 14 Publications |
|
D'Angelo, Vincenza, et al. "Phosphodiesterase-10A inverse changes in striatopallidal and striatoentopeduncular pathways of a transgenic mouse model of DYT1 dystonia." Journal of Neuroscience 37.8 (2017) PMID28115486 |
Immunocytochemistry, Western Blot; Mouse |
Harada, Akina, et al. "Characterization of the binding properties of T-773 as a PET radioligand for phosphodiesterase 10A." Nuclear medicine and biology 42.2 (2015): 146-154. PMID25451212 |
Western Blot; Monkey |
Mango, Dalila, et al. "Phosphodiesterase 10A controls D1-mediated facilitation of GABA release from striato-nigral projections under normal and dopamine-depleted conditions." Neuropharmacology 76 (2014): 127-136. PMID23973317 |
Western Blot; Mouse |
Sancesario, Giuseppe, et al. "Levodopa-induced dyskinesias are associated with transient down-regulation of cAMP and cGMP in the caudate-putamen of hemiparkinsonian rats: Reduced synthesis or increased catabolism?." Neurochemistry international 79 (2014): 44-56. PMID25452081 |
Western Blot; Rat |
Nishi, Akinori et al. “Distinct Roles of PDE4 and PDE10A in the Regulation of cAMP/PKA Signaling in the Striatum.” The Journal of neuroscience?: the official journal of the Society for Neuroscience 28.42 (2008): 10460. PMC2814340 |
Immunohistochemistry; Mouse |
Heine, C., et al. "Phosphodiesterase 2 inhibitors promote axonal outgrowth in organotypic slice co-cultures." Neurosignals 21.3/4 (2013): 197-212. PMID22947663 |
Immunohistochemistry, Immunofluorescence; Rat |
Giorgi, M., et al. "PDE10A and PDE10A-dependent cAMP catabolism are dysregulated oppositely in striatum and nucleus accumbens after lesion of midbrain dopamine neurons in Rat: a key step in parkinsonism physiopathology." Neurobiology of disease 43.1 (2011): 293-303.PMID21515371 |
Immunohistochemistry; Mouse |
Ong, WK et al. “The Role of the PDE4D cAMP Phosphodiesterase in the Regulation of Glucagon-Like Peptide-1 Release.” British Journal of Pharmacology 157.4 (2009): 633–644. PMC2707975 |
Immunofluorescence, Western Blot; Mouse |
Kruse, Lars S., et al. "PDE9A, PDE10A, and PDE11A expression in Rat trigeminovascular pain signalling system." Brain research 1281 (2009): 25-34. PMID19445908 |
Immunohistochemistry, Immunodepletion, Western Blot; Rat |
Dlaboga, Daniel, Hassan Hajjhussein, and James M. O'Donnell. "Chronic haloperidol and clozapine produce different patterns of effects on phosphodiesterase-1B,-4B, and-10A expression in Rat striatum." Neuropharmacology 54.4 (2008): 745-754. PMID18222493 |
Western Blot; Rat |
Bora, Roop Singh, et al. "Development of a cell‐based assay for screening of phosphodiesterase 10A (PDE10A) inhibitors using a stable recombinant HEK‐293 cell line expressing high levels of PDE10A." Biotechnology and applied biochemistry 49.2 (2008): 129-134. PMID17640173 |
Immunocytochemistry, Western Blot; Human |
Albrecht, Knut, et al. "Immunohistochemical distribution of cyclic nucleotide phosphodiesterase (PDE) isoenzymes in the Human vagina: A potential forensic value?." Journal of forensic and legal medicine 14.5 (2007): 270-274. PMID17113813 |
Immunohistochemistry; Human |
Ückert, Stefan, et al. "Immunohistochemical Description of Cyclic Nucleotide Phosphodiesterase (PDE) Isoenzymes in the Human Labia Minora." The Journal of Sexual Medicine 4.3 (2007): 602-608.PMID17498099 |
Immunofluorescence; Human |
Ückert, Stefan, et al. "Cyclic adenosine monophosphate and cyclic guanosine monophosphate-phosphodiesterase isoenzymes in Human vagina: relation to nitric oxide synthase isoforms and vasoactive intestinal polypeptide-containing nerves." Urology 65.3 (2005): 604-610. PMID15780401 |
Immunohistochemistry; Human |
Publishing research using PD10A-101AP? Please let us know, we can cite the reference in this datasheet.
Product | Note | Status | Price | |
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PD10A-101AP | |||
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P-PD10A | |||
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PD10A-BIOTIN | |||
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PC-PD10A | |||
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