General Information

Product

MAP3K4 Antibody

Description

Affinity Purified Mitogen-activated protein kinase kinase kinase 4 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1558-1608 on human MAP3K4 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9Y6R4

Overview

Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm > perinuclear region

Expression

Expressed at high levels in heart, placenta, skeletal muscle and pancreas, and at lower levels in other tissues.

Structure

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain.

Alternative Nomenclature

dJ473J16.1 (mitogen-activated protein kinase kinase kinase 4) antibody

FLJ42439 antibody

KIAA0213 antibody

MAP three kinase 1 antibody

MAP/ERK kinase kinase 4 antibody

MAP3K4 antibody

MAPKKK4 antibody

MEK kinase 4 antibody

MEKK4 antibody

Mitogen-activated protein kinase kinase kinase 4 antibody

MTK1 antibody

predicted protein of HQ0412 antibody

PRO0412 antibody

RP3-473J16.4 antibody

SSK2/SSK22 MAP kinase kinase kinase, yeast, homolog of antibody

General Information

Product

MEK1 Antibody (MAP2K1)

Description

Affinity Purified Mitogen-activated protein kinase kinase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-350 on human Mitogen-activated protein kinase kinase 1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q02750

Overview

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Subcellular Location

Cytoplasm, Cytoskeleton, Membrane, Nucleus

Expression

Widely expressed, with extremely low levels in brain.

Structure

The proline-rich region localized between residues 270 and 307 is important for binding to RAF1 and activation of MAP2K1/MEK1.

Alternative Nomenclature

Dual specificity mitogen activated protein kinase kinase 1 antibody
ERK activator kinase 1 antibody
MAP kinase/Erk kinase 1 antibody
MAP2K1 antibodyMAPKK 1 antibody
MAPKK1 antibody
MEKK1 antibody
Mitogen activated protein kinase kinase 1 antibody
MKK1 antibodyPRKMK1 antibody

Protein kinase mitogen activated, kinase 1 antibody

General Information

Product

MAPK1 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-350 on human Mitogen-activated protein kinase 1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P28482

Overview

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis.

Molecular Function

Kinase, Repressor, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm › cytoskeleton › spindle

Nucleus

Cytoplasm › cytoskeleton › microtubule organizing center › centrosome

Cytoplasm

Structure

Binds both upstream activators and downstream substrates in multimolecular complexes. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with ADAM15, ARHGEF2, ARRB2, DAPK1 (via death domain), HSF4,

Alternative Nomenclature

ERK antibody
ERK-2 antibody
ERT1 antibody
Extracellular signal-regulated kinase 2 antibody
MAP kinase 1 antibody

MAP kinase isoform p42 antibody
MAPK 1 antibody

Mapk1 antibody
MAPK2 antibody
Mitogen-activated protein kinase 1 antibody
P38 antibody
P40 antibody
P41 antibody
p42-MAPK antibody
P42MAPK antibody
PRKM1 antibody
protein kinase, mitogen-activated, 1 antibody

General Information

Product

MAPK10 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 10 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 375-425 on human Mitogen-activated protein kinase 10.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P53779

Overview

Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Membrane, Mitochondrion, Nucleus

Expression

Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.

Structure

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Alternative Nomenclature

c Jun kinase 3 antibody
c-Jun N-terminal kinase 3 antibody
JNK3 alpha protein kinase antibody
JNK3 antibody
JNK3A antibody
MAP kinase 10 antibody
MAP kinase p49 3F12 antibody
MAPK 10 antibody
mitogen activated protein kinase 10 antibody
PRKM10 antibody
protein kinase mitogen activated 10 antibody
SAPK1b antibody
Stress activated protein kinase 1b antibody
stress activated protein kinase beta antibody
Stress activated protein kinase JNK3 antibody

General Information

Product

MAPK11 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 11 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-350 on human Mitogen-activated protein kinase 11.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q15759

Overview

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK11 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK11 functions are mostly redundant with those of MAPK14. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Nucleus

Expression

Highest levels in the brain and heart. Also expressed in the placenta, lung, liver, skeletal muscle, kidney and pancreas.

Structure

Interacts with HDAC3 and DUSP16.

Alternative Nomenclature

Human p38Beta MAP kinase mRNA complete cds antibody
MAP kinase 11 antibody
MAP kinase p38 beta antibody
Mitogen activated protein kinase 11 antibody
Mitogen activated protein kinase p38 2 antibody
Mitogen-activated protein kinase p38 beta antibody
MK11_HUMAN antibody
p38Beta antibody
PRKM11 antibody
Protein kinase mitogen activated 11 antibody
SAPK2B antibody
Stress activated protein kinase 2b antibody

General Information

Product

MAPK12 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 12 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 310-360 on human Mitogen-activated protein kinase 12.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P53778

Overview

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Mitochondrion, Nucleus

Expression

Highly expressed in skeletal muscle and heart.

Structure

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Alternative Nomenclature

ERK 3 antibody
ERK 5 antibody
ERK 6 antibody=Extracellular signal-regulated kinase 6 antibody
MAP kinase 12 antibody
MAP kinase p38 gamma antibody

Mitogen Activated Protein Kinase 12 antibody
Mitogen activated protein kinase 3 antibody

P38 GAMMA antibody

PRKM12 antibody
SAPK 3 antibody

Stress Activated Protein Kinase 3 antibody

General Information

Product

MAPK13 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 13 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 310-360 on human Mitogen-activated protein kinase 13.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O15264

Overview

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK13 is one of the less studied p38 MAPK isoforms.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytosol

Expression

Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells.

Structure

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Alternative Nomenclature

MAP kinase 13 antibody

MAPK 13 antibody

Mitogen activated protein kinase 13 antibody
p38 delta antibody

PRKM13 antibody
SAPK 4 antibody
Stress activated protein kinase 4 antibody

General Information

Product

MAPK3 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 3 Antibody

Verified Applications

ELISA, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-350 on human Mitogen-activated protein kinase 3.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P27361

Overview

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Nucleus

Structure

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Alternative Nomenclature

Mitogen-activated protein kinase 3 Antibody MAP kinase 3 Antibody

MAPK 3 Antibody

ERT2 Antibody

Extracellular signal-regulated kinase 1 Antibody ERK-1 Antibody

Insulin-stimulated MAP2 kinase Antibody

MAP kinase isoform p44 Antibody p44-MAPK Antibody Microtubule-associated protein 2 kinase Antibody

p44-ERK1 Antibody

MAPK3 Antibody

ERK1 Antibody

PRKM3 Antibody

General Information

Product

MAPK4 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 4 Antibody

Verified Applications

ELISA, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 500-550 on human Mitogen-activated protein kinase 4.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P31152

Overview

Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Nucleus

Expression

High expression in heart and brain.

Structure

Homodimer. Heterodimer with ERK3/MAPK6. Interacts with (via FRIEDE motif) MAPKAPK5.

Alternative Nomenclature

ERK-4 antibody
Extracellular signal-regulated kinase 4 antibody
MAP kinase 4 antibody
MAP kinase isoform p63 antibody

MAPK4 antibody

p63 MAPK antibody

PRKM4 antibody
Protein kinase, mitogen-activated, 4 antibody
Protein-tyrosine kinase ERK3-related antibody
RMNK2 antibody

General Information

Product

MAPK6 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 6 Antibody

Verified Applications

ELISA, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 480-530 on human Mitogen-activated protein kinase 6.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q16659

Overview

Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Nucleus

Expression

Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.

Structure

In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases.

Alternative Nomenclature

ERK3 antibody
Extracellular signal regulated kinase p97 antibody
Extracellular signal-regulated kinase 3 antibody
HsT17250 antibody
MAP kinase 6 antibody
MAP kinase isoform p97 antibody
Mitogen activated protein kinase 6 antibody
p97 MAPK antibody
PRKM6 antibody
Protein kinase mitogen activated 6 antibody